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. 1971 Jul;123(4):571–577. doi: 10.1042/bj1230571

The transport of citrate and other tricarboxylic acids in two species of Pseudomonas

H G Lawford 1, G R Williams 1
PMCID: PMC1176996  PMID: 5126909

Abstract

When cells of Pseudomonas are grown on citrate as the sole carbon source they oxidize citrate and isocitrate rapidly. Fluorocitrate inhibits the oxidation of citrate. Fluorocitrate-treated cells accumulate [6-14C]citrate, as shown by a rapid Millipore-filtration technique. In the absence of fluorocitrate most of the [6-14C]-citrate is lost in the form of 14CO2. The isolation of a pseudomonad characterized by its ability to grow on tricarballylate as a sole carbon source has facilitated the study of the tricarboxylate-carrier specificity. Cells grown on citrate will exchange radioactive citrate for unlabelled citrate or isocitrate but not for cis-aconitate, trans-aconitate or tricarballylate. Cells grown on tricarballylate will exchange radioactive citrate for unlabelled citrate, cis-aconitate or tricarballylate, but not for isocitrate or trans-aconitate. The properties of the exchange system involved are compared with those of the related system in mitochondria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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