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. 1973 May;133(1):37–47. doi: 10.1042/bj1330037

The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum

D Every 1, J M Ashworth 1
PMCID: PMC1177668  PMID: 4198517

Abstract

The purification of β-N-acetylhexosaminidase, α-glucosidase, α-mannosidase and β-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. β-N-Acetylhexosaminidase and α-glucosidase were obtained in high yield and as homogeneous preparations whereas the α-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described.

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Selected References

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