Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1973 Jun;133(2):405–408. doi: 10.1042/bj1330405

Nitrogenase of Klebsiella pneumoniae: evidence for an adenosine triphosphate-induced association of the iron–sulphur protein (Short Communication)

Roger N F Thorneley 1, Robert R Eady 1
PMCID: PMC1177714  PMID: 4579395

Abstract

The effect of various nucleotides on the Fe-containing component of nitrogenase of Klebsiella pneumoniae was investigated by ultracentrifugation and thiol-group reactivity towards 5,5′-dithiobis-(2-nitrobenzoate). In the absence of Na2S2O4, ATP and ADP produced changes in sedimentation behaviour and thiol-group reactivity consistent with association of the protein.

Full text

PDF
406

Images in this article

407Fig. 2.
on p.407

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biggins D. R., Kelly M. Interaction of nitrogenase from Klebsiella pneumoniae with ATP or cyanide. Biochim Biophys Acta. 1970;205(2):288–299. doi: 10.1016/0005-2728(70)90258-6. [DOI] [PubMed] [Google Scholar]
  2. Eady R. R., Smith B. E., Cook K. A., Postgate J. R. Nitrogenase of Klebsiella pneumoniae. Purification and properties of the component proteins. Biochem J. 1972 Jul;128(3):655–675. doi: 10.1042/bj1280655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hardy R. W., Holsten R. D., Jackson E. K., Burns R. C. The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation. Plant Physiol. 1968 Aug;43(8):1185–1207. doi: 10.1104/pp.43.8.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Moustafa E., Mortenson L. E. Properties of azoferredoxin purified from nitrogen-fixing extracts of Clostridium pasteurianum. Biochim Biophys Acta. 1969 Jan 14;172(1):106–115. doi: 10.1016/0005-2728(69)90095-4. [DOI] [PubMed] [Google Scholar]
  5. Mustafa E., Mortenson L. E. Acetylene reduction by nitrogen fixing extracts of Clostridium pasteurianum: ATP requirement and inhibition by ADP. Nature. 1967 Dec 23;216(5121):1241–1242. doi: 10.1038/2161241a0. [DOI] [PubMed] [Google Scholar]
  6. Orme-Johnson W. H., Hamilton W. D., Jones T. L., Tso M. Y., Burris R. H., Shah V. K., Brill W. J. Electron paramagnetic resonance of nitrogenase and nitrogenase components from Clostridium pasteurianum W5 and Azotobacter vinelandii OP. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3142–3145. doi: 10.1073/pnas.69.11.3142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Smith B. E., Lowe D. J., Bray R. C. Nitrogenase of Klebsiella pneumoniae: electron-paramagnetic-resonance studies on the catalytic mechanism. Biochem J. 1972 Nov;130(2):641–643. doi: 10.1042/bj1300641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Yates M. G. The effect of ATP upon the oxygen sensitivity of nitrogenase from Azotobacter chroococcum. Eur J Biochem. 1972 Sep 18;29(2):386–392. doi: 10.1111/j.1432-1033.1972.tb02000.x. [DOI] [PubMed] [Google Scholar]
  9. Zumft W. G., Cretney W. C., Huang T. C., Mortenson L. E., Palmer G. On the structure and function of nitrogenase from Clostridium pasteurianum W5. Biochem Biophys Res Commun. 1972 Sep 26;48(6):1525–1532. doi: 10.1016/0006-291x(72)90887-x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES