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. 1973 Jun;134(2):581–586. doi: 10.1042/bj1340581

A model study of the fructose diphosphatase–phosphofructokinase substrate cycle

David P Bloxham 1, Michael G Clark 1, Paul C Holland 1, Henry A Lardy 1
PMCID: PMC1177845  PMID: 16742819

Abstract

A fructose diphosphatase–phosphofructokinase substrate cycle has been reconstructed in vitro to provide a system that recycles fructose 6-phosphate and hydrolyses ATP to ADP and Pi. The concerted actions of glucose phosphate isomerase, phosphofructokinase, aldolase and triose phosphate isomerase catalysed the loss of 3H from [5-3H,U-14C]glucose 6-phosphate. This was used as the basis of a method for the estimation of the fructose diphosphatase–phosphofructokinase substrate cycle. For the reconstructed cycle, the rate of decrease of the 3H/14C ratio in [5-3H,U-14C]hexose 6-phosphate was proportional to the rate of fructose 6-phosphate substrate cycling. A detailed theoretical treatment of this relationship is developed, which enables the rate of substrate cycling to be determined in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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