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. 1973 Jun;134(2):647–650. doi: 10.1042/bj1340647

The uptake of [45Ca]calcium ions by matrix vesicles isolated from calcifying cartilage (Short Communication)

S Y Ali 1, Lois Evans 1
PMCID: PMC1177853  PMID: 16742827

Abstract

Extracellular membranous matrix vesicles, which contain various phosphatases and appear to initiate hydroxyapatite formation in growth cartilage, were isolated and incubated with 45Ca2+ and shown to form mineral in the presence of ATP. There is enhanced calcification in the presence of serum and under alkaline conditions.

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Selected References

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  1. Agus S. G., Cox R. P., Griffin M. J. Inhibition of alkaline phosphatase by cysteine and its analogues. Biochim Biophys Acta. 1966 May 5;118(2):363–370. doi: 10.1016/s0926-6593(66)80045-0. [DOI] [PubMed] [Google Scholar]
  2. Ali S. Y., Evans L. Studies on the cathepsins in elastic cartilage. Biochem J. 1969 May;112(4):427–433. doi: 10.1042/bj1120427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ali S. Y., Sajdera S. W., Anderson H. C. Isolation and characterization of calcifying matrix vesicles from epiphyseal cartilage. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1513–1520. doi: 10.1073/pnas.67.3.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Anderson H. C. Electron microscopic studies of induced cartilage development and calcification. J Cell Biol. 1967 Oct;35(1):81–101. doi: 10.1083/jcb.35.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Anderson H. C., Matsuzawa T., Sajdera S. W., Ali S. Y. Membranous particles in calcifying cartilage matrix. Trans N Y Acad Sci. 1970 May;32(5):619–630. doi: 10.1111/j.2164-0947.1970.tb02737.x. [DOI] [PubMed] [Google Scholar]
  6. Anderson H. C. Vesicles associated with calcification in the matrix of epiphyseal cartilage. J Cell Biol. 1969 Apr;41(1):59–72. doi: 10.1083/jcb.41.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bernard G. W. The ultrastructural interface of bone crystals and organic matrix in woven and lamellar endochondral bone. J Dent Res. 1969 Sep-Oct;48(5):781–788. doi: 10.1177/00220345690480052901. [DOI] [PubMed] [Google Scholar]
  8. Bernard G. W. Ultrastructural observations of initial calcification in dentine and enamel. J Ultrastruct Res. 1972 Oct;41(1):1–17. doi: 10.1016/s0022-5320(72)90034-2. [DOI] [PubMed] [Google Scholar]
  9. Bonucci E. Fine structure and histochemistry of "calcifying globules" in epiphyseal cartilage. Z Zellforsch Mikrosk Anat. 1970;103(2):192–217. doi: 10.1007/BF00337312. [DOI] [PubMed] [Google Scholar]
  10. Bonucci E. Fine structure of early cartilage calcification. J Ultrastruct Res. 1967 Sep;20(1):33–50. doi: 10.1016/s0022-5320(67)80034-0. [DOI] [PubMed] [Google Scholar]
  11. Bowness J. M. Present concepts of the role of ground substance in calcification. Clin Orthop Relat Res. 1968 Jul-Aug;59:233–247. [PubMed] [Google Scholar]
  12. Brookes M., Richards D. J., Singh M. Vascular sequelae of experimental osteotomy. Angiology. 1970 Jun;21(6):355–367. doi: 10.1177/000331977002100601. [DOI] [PubMed] [Google Scholar]
  13. CARTIER P. Mécanisme enzymatique de l'ossification. Expos Annu Biochim Med. 1952;14:73–86. [PubMed] [Google Scholar]
  14. CARTIER P., PICARD J. La minéralisation du cartilage ossifiable. II. Le système ATPasique du cartilage. Bull Soc Chim Biol (Paris) 1955;37(5-6):661–675. [PubMed] [Google Scholar]
  15. Fernley H. N., Bisaz S. Studies on alkaline phosphatase. Phosphorylation of calf-intestinal alkaline phosphatase by 32P-labelled pyrophosphate. Biochem J. 1968 Mar;107(2):279–283. doi: 10.1042/bj1070279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Fernley H. N., Walker P. G. Studies on alkaline phosphatase. Inhibition by phosphate derivatives and the substrate specificity. Biochem J. 1967 Sep;104(3):1011–1018. doi: 10.1042/bj1041011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. HASSELBACH W., MAKINOSE M. [The calcium pump of the "relaxing granules" of muscle and its dependence on ATP-splitting]. Biochem Z. 1961;333:518–528. [PubMed] [Google Scholar]
  18. Howell D. S., Pita J. C., Marquez J. F., Madruga J. E. Partition of calcium, phosphate, and protein in the fluid phase aspirated at calcifying sites in epiphyseal cartilage. J Clin Invest. 1968 May;47(5):1121–1132. doi: 10.1172/JCI105801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Irving J. T., Wuthier R. E. Histochemistry and biochemistry of calcification with special reference to the role of lipids. Clin Orthop Relat Res. 1968 Jan-Feb;56:237–260. [PubMed] [Google Scholar]
  20. Jibril A. O. Phosphates and phosphatases in preosseous cartilage. Biochim Biophys Acta. 1967 Aug 29;141(3):605–613. doi: 10.1016/0304-4165(67)90189-4. [DOI] [PubMed] [Google Scholar]
  21. Kim K. M., Huang S. N. Ultrastructural study of calcification of human aortic valve. Lab Invest. 1971 Nov;25(5):357–366. [PubMed] [Google Scholar]
  22. Lehninger A. L. Mitochondria and calcium ion transport. Biochem J. 1970 Sep;119(2):129–138. doi: 10.1042/bj1190129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lieberman E. M., Palmer R. F., Collins G. H. Calcium ion uptake by crustacean peripheral nerve subcellular particles. Exp Cell Res. 1967 May;46(2):412–418. doi: 10.1016/0014-4827(67)90077-8. [DOI] [PubMed] [Google Scholar]
  24. MARTONOSI A., FERETOS R. SARCOPLASMIC RETICULUM. I. THE UPTAKE OF CA++ BY SARCOPLASMIC RETICULUM FRAGMENTS. J Biol Chem. 1964 Feb;239:648–658. [PubMed] [Google Scholar]
  25. Matsuzawa T., Anderson H. C. Phosphatases of epiphyseal cartilage studied by electron microscopic cytochemical methods. J Histochem Cytochem. 1971 Dec;19(12):801–808. doi: 10.1177/19.12.801. [DOI] [PubMed] [Google Scholar]
  26. Matthews J. L., Martin J. H., Lynn J. A., Collins E. J. Calcium incorporation in the developing cartilaginous epiphysis. Calcif Tissue Res. 1968 Mar 27;1(4):330–336. doi: 10.1007/BF02008105. [DOI] [PubMed] [Google Scholar]
  27. Russell R. G., Monod A., Bonjour J. P., Fleisch H. Relation between alkaline phosphatase and Ca 2+ -ATPase in calcium transport. Nat New Biol. 1972 Nov 22;240(99):126–127. doi: 10.1038/newbio240126a0. [DOI] [PubMed] [Google Scholar]

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