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. 1972 Jan;126(1):187–191. doi: 10.1042/bj1260187

The enzymic degradation of l-serine O-sulphate by a specific system from pig liver. Studies on the mechanism of the reaction

N Tudball 1, P Thomas 1
PMCID: PMC1178363  PMID: 5075228

Abstract

1. By using [18O]water it was demonstrated that l-serine O-sulphate undergoes C–O cleavage during enzyme-catalysed degradations. 2. C–O cleavage of substrate also occurs under the agency of alkali and pyridoxal phosphate, but, as expected, acid-catalysed degradation involves S–O scission. 3. Aminoacrylate was identified as an intermediate in the enzyme-catalysed degradation of l-serine O-sulphate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. John R. A., Fasella P. The reaction of L-serine O-sulfate with aspartate aminotransferase. Biochemistry. 1969 Nov;8(11):4477–4482. doi: 10.1021/bi00839a038. [DOI] [PubMed] [Google Scholar]
  2. SPENCER B. Studies on sulphatases. 20. Enzymic cleavage of aryl hydrogen sulphates in the presence of H218O. Biochem J. 1958 May;69(1):155–159. doi: 10.1042/bj0690155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Spencer B. Studies on sulphatases. 25. The determination of BaSO(3)O by infrared spectroscopy. Biochem J. 1959 Nov;73(3):442–447. doi: 10.1042/bj0730442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. TONHAZY N. E., WHITE N. G., UMBREIT W. W. A rapid method for the estimation of the glutamic-aspartic transaminase in tissues and its application to radiation sickness. Arch Biochem. 1950 Aug;28(1):36–42. [PubMed] [Google Scholar]
  5. Tate S. S., Relyea N. M., Meister A. Interaction of L-aspartate beta-decarboxylase with beta-chloro-L-alanine. Beta-elimination reaction and active-site labeling. Biochemistry. 1969 Dec;8(12):5016–5021. doi: 10.1021/bi00840a051. [DOI] [PubMed] [Google Scholar]
  6. Thomas J. H., Tudball N. Studies on the enzymic degradation of L-serine O-sulphate by a rat liver preparation. Biochem J. 1967 Nov;105(2):467–472. doi: 10.1042/bj1050467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Tudball N. The metabolism of potassium l-serine O[S]-sulphate in the rat. Biochem J. 1962 Dec;85(3):456–460. doi: 10.1042/bj0850456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tudball N., Thomas P., Bailey-Wood R. The purification and properties of the L-serine O-sulphate degrading system of pig liver. Biochem J. 1971 Mar;121(5):747–752. doi: 10.1042/bj1210747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tudballn, Thmas J. H., Fowler J. A. A rat liver system that catalyses a pyridoxal phosphate-independent alpha beta-eliminto. Biochem J. 1969 Sep;114(2):299–305. doi: 10.1042/bj1140299. [DOI] [PMC free article] [PubMed] [Google Scholar]

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