Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1972 Jan;126(1):187–191. doi: 10.1042/bj1260187

The enzymic degradation of l-serine O-sulphate by a specific system from pig liver. Studies on the mechanism of the reaction

N Tudball 1, P Thomas 1
PMCID: PMC1178363  PMID: 5075228

Abstract

1. By using [18O]water it was demonstrated that l-serine O-sulphate undergoes C–O cleavage during enzyme-catalysed degradations. 2. C–O cleavage of substrate also occurs under the agency of alkali and pyridoxal phosphate, but, as expected, acid-catalysed degradation involves S–O scission. 3. Aminoacrylate was identified as an intermediate in the enzyme-catalysed degradation of l-serine O-sulphate.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. John R. A., Fasella P. The reaction of L-serine O-sulfate with aspartate aminotransferase. Biochemistry. 1969 Nov;8(11):4477–4482. doi: 10.1021/bi00839a038. [DOI] [PubMed] [Google Scholar]
  2. SPENCER B. Studies on sulphatases. 20. Enzymic cleavage of aryl hydrogen sulphates in the presence of H218O. Biochem J. 1958 May;69(1):155–159. doi: 10.1042/bj0690155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Spencer B. Studies on sulphatases. 25. The determination of BaSO(3)O by infrared spectroscopy. Biochem J. 1959 Nov;73(3):442–447. doi: 10.1042/bj0730442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. TONHAZY N. E., WHITE N. G., UMBREIT W. W. A rapid method for the estimation of the glutamic-aspartic transaminase in tissues and its application to radiation sickness. Arch Biochem. 1950 Aug;28(1):36–42. [PubMed] [Google Scholar]
  5. Tate S. S., Relyea N. M., Meister A. Interaction of L-aspartate beta-decarboxylase with beta-chloro-L-alanine. Beta-elimination reaction and active-site labeling. Biochemistry. 1969 Dec;8(12):5016–5021. doi: 10.1021/bi00840a051. [DOI] [PubMed] [Google Scholar]
  6. Thomas J. H., Tudball N. Studies on the enzymic degradation of L-serine O-sulphate by a rat liver preparation. Biochem J. 1967 Nov;105(2):467–472. doi: 10.1042/bj1050467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Tudball N. The metabolism of potassium l-serine O[S]-sulphate in the rat. Biochem J. 1962 Dec;85(3):456–460. doi: 10.1042/bj0850456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tudball N., Thomas P., Bailey-Wood R. The purification and properties of the L-serine O-sulphate degrading system of pig liver. Biochem J. 1971 Mar;121(5):747–752. doi: 10.1042/bj1210747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tudballn, Thmas J. H., Fowler J. A. A rat liver system that catalyses a pyridoxal phosphate-independent alpha beta-eliminto. Biochem J. 1969 Sep;114(2):299–305. doi: 10.1042/bj1140299. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES