Abstract
The plasma specific radioactivity of arginine guanidine 14C-labelled protein has been measured at various times after the injection of labelled protein precursor (Na214CO3). The resulting `appearance' curve is a balance between the rate of synthesis and delivery of new protein and the rate of destruction and distribution of new plus existing protein. The measured appearance curve can be corrected to give a measure solely of the rate of synthesis of the plasma protein concerned, if the amplitude of the curve at every point is increased by an amount that compensates for the destruction and distribution processes. The decrease of plasma specific radioactivity of labelled plasma protein after an injection of exogenously labelled protein is termed the `disappearance' curve, and the method of correcting the appearance curve by using the disappearance curve is shown to take the form of a deconvolution.
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Selected References
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- MATTHEWS C. M. The theory of tracer experiments with 131I-labelled plasma proteins. Phys Med Biol. 1957 Jul;2(1):36–53. doi: 10.1088/0031-9155/2/1/305. [DOI] [PubMed] [Google Scholar]
- Norwich K. H., Hetenyi G., Jr Basic studies on metabolic steady state. Linearity of a metabolic tracer system. Biophysik. 1971;7(3):169–180. doi: 10.1007/BF01190723. [DOI] [PubMed] [Google Scholar]