Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1972 May;127(5):901–905. doi: 10.1042/bj1270901

Folate metabolism in Streptococcus faecalis

P G McElwee 1, J M Scott 1
PMCID: PMC1178800  PMID: 4627689

Abstract

The possibility that the inability of Streptococcus faecalis to utilize 5-methyltetrahydropteroylglutamate or pteroyltriglutamate might be due to permeability was investigated. Whereas the former was taken up by S. faecalis cells growing on pteroylglutamic acid, the latter was not. No subsequent conversion of the 5-methyltetrahydropteroylglutamate took place and accumulation, which was against a considerable concentration gradient, was inhibited by fluoride. It would thus appear to be an active process.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKERMAN H. A. A method for measuring the microbiological activity of tetrahydrofolic acid and other labile reduced folic acid derivatives. Anal Biochem. 1961 Dec;2:558–567. doi: 10.1016/0003-2697(61)90023-9. [DOI] [PubMed] [Google Scholar]
  2. Baugh C. M., Stevens J. C., Krumdieck C. L. Studies on gamma-glutamyl carboxypeptidase. I. The solid phase synthesis of analogs of polyglutamates of folic acid and their effects on human liver gamma-glutamyl carboxypeptidase. Biochim Biophys Acta. 1970 Jul 15;212(1):116–125. doi: 10.1016/0005-2744(70)90184-1. [DOI] [PubMed] [Google Scholar]
  3. Blair J. A., Saunders K. J. A convenient method for the preparation of dl-5-methyltetrahydrofolic acid (dl-5-methyl-5,6,7,8-tetrahydropteroyl-L-monoglutamic acid). Anal Biochem. 1970 Apr;34(2):376–381. doi: 10.1016/0003-2697(70)90122-3. [DOI] [PubMed] [Google Scholar]
  4. Davis R. E., Nicol D. J., Kelly A. An automated method for the measurement of folate activity. J Clin Pathol. 1970 Feb;23(1):47–53. doi: 10.1136/jcp.23.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HERBERT V., LARRABEE A. R., BUCHANAN J. M. Studies on the identification of a folate compound of human serum. J Clin Invest. 1962 May;41:1134–1138. doi: 10.1172/JCI104565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HERBERT V. The assay and nature of folic acid activity in human serum. J Clin Invest. 1961 Jan;40:81–91. doi: 10.1172/JCI104240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JOHNS D. G., BERTINO J. R. FOLATES AND MEGALOBLASTIC ANEMIA: A REVIEW. Clin Pharmacol Ther. 1965 May-Jun;6:372–392. doi: 10.1002/cpt196563372. [DOI] [PubMed] [Google Scholar]
  8. Koch A. L. Turbidity measurements of bacterial cultures in some available commercial instruments. Anal Biochem. 1970 Nov;38(1):252–259. doi: 10.1016/0003-2697(70)90174-0. [DOI] [PubMed] [Google Scholar]
  9. Krumdieck C. L., Baugh C. M. The solid-phase synthesis of polyglutamates of folic acid. Biochemistry. 1969 Apr;8(4):1568–1572. doi: 10.1021/bi00832a036. [DOI] [PubMed] [Google Scholar]
  10. Mandelbaum-Shavit F., Grossowicz N. Transport of folinate and related compounds in Pediococcus cerevisiae. J Bacteriol. 1970 Oct;104(1):1–7. doi: 10.1128/jb.104.1.1-7.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Perry J., Chanarin I. Intestinal absorption of reduced folate compounds in man. Br J Haematol. 1970 Mar;18(3):329–339. doi: 10.1111/j.1365-2141.1970.tb01447.x. [DOI] [PubMed] [Google Scholar]
  12. WOOD R. C., HITCHINGS G. H. A study of the uptake and degradation of folic acid, citrovorum factor, aminopterin, and pyrimethamine by bacteria. J Biol Chem. 1959 Sep;234:2381–2385. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES