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. 1970 Mar;117(1):183–192. doi: 10.1042/bj1170183

The amino acid sequence of Phaseolus aureus L. (mung-bean) cytochrome c

E W Thompson 1, M V Laycock 1,*, J A M Ramshaw 1, D Boulter 1
PMCID: PMC1178845  PMID: 5420954

Abstract

The amino acid sequence of Phaseolus aureus L. (mung-bean) cytochrome c has been determined. The molecule consists of a single polypeptide chain of 111 amino acid residues and is homologous with other mitochondrial cytochromes c. Comparison with the amino acid sequence of wheat-germ cytochrome c (Stevens, Glazer & Smith, 1967) shows 14 differences. On alignment with mammalian cytochromes c, mung-bean cytochrome c has an N-acetylated `tail' of eight amino acid residues similar to that found in wheat-germ cytochrome c. Of the 22 positions in wheat-germ cytochrome c that contain amino acid residues unique to these positions, 20 were found to contain the same ones in mung-bean cytochrome c. The ∈-N-trimethyl-lysine residues reported for wheat-germ cytochrome c (Delange, Glazer & Smith, 1969) in positions 72 and 86 were also found in these positions in mung-bean cytochrome c. The sequence was determined from 3μmol, by using chymotryptic and tryptic peptides which were analysed by the `dansyl'–Edman method (Gray & Hartley, 1963a), with confirmation by amino acid analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMBLER R. P. THE PURIFICATION AND AMINO ACID COMPOSITION OF PSEUDOMONAS CYTOCHROME C-551. Biochem J. 1963 Nov;89:341–349. doi: 10.1042/bj0890341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DeLange R. J., Glazer A. N., Smith E. L. Presence and location of an unusual amino acid, epsilon-N-trimethyllysine, in cytochrome c of wheat germ and Neurospora. J Biol Chem. 1969 Mar 10;244(5):1385–1388. [PubMed] [Google Scholar]
  3. Easley C. W. Combinations of specific color reactions useful in the peptide mapping technique. Biochim Biophys Acta. 1965 Sep 13;107(2):386–388. doi: 10.1016/0304-4165(65)90147-9. [DOI] [PubMed] [Google Scholar]
  4. Fitch W. M., Margoliash E. Construction of phylogenetic trees. Science. 1967 Jan 20;155(3760):279–284. doi: 10.1126/science.155.3760.279. [DOI] [PubMed] [Google Scholar]
  5. GRAY W. R., HARTLEY B. S. THE STRUCTURE OF A CHYMOTRYPTIC PEPTIDE FROM PSEUDOMONAS CYTOCHROME C-551. Biochem J. 1963 Nov;89:379–380. doi: 10.1042/bj0890379. [DOI] [PubMed] [Google Scholar]
  6. Gros C., Labouesse B. Study of the dansylation reaction of amino acids, peptides and proteins. Eur J Biochem. 1969 Feb;7(4):463–470. doi: 10.1111/j.1432-1033.1969.tb19632.x. [DOI] [PubMed] [Google Scholar]
  7. HEILMANN J., BARROLLIER J., WATZKE E. Beitrag zur Aminosäurebestimmung auf Papierchromatogrammen. Hoppe Seylers Z Physiol Chem. 1957;309(4-6):219–220. [PubMed] [Google Scholar]
  8. MARGOLIASH E., KIMMEL J. R., HILL R. L., SCHMIDT W. R. Amino acid composition of horse heart cytochrome c. J Biol Chem. 1962 Jul;237:2148–2150. [PubMed] [Google Scholar]
  9. MARGOLIASH E., SMITH E. L., KREIL G., TUPPY H. Amino-acid sequence of horse heart cytochrome c. Nature. 1961 Dec 23;192:1125–1127. doi: 10.1038/1921125a0. [DOI] [PubMed] [Google Scholar]
  10. Matsubara H., Sasaki R. M. High recovery of tryptophan from acid hydrolysates of proteins. Biochem Biophys Res Commun. 1969 Apr 29;35(2):175–181. doi: 10.1016/0006-291x(69)90263-0. [DOI] [PubMed] [Google Scholar]
  11. Nolan C., Margoliash E. Primary structure of the cytochrome c from the great grey kangaroo, Macropus canguru. J Biol Chem. 1966 Mar 10;241(5):1049–1059. [PubMed] [Google Scholar]
  12. Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
  13. STEWART I. Determination of ornithine, lysine, arginine, citrulline and histidine. J Chromatogr. 1963 Mar;10:404–407. doi: 10.1016/s0021-9673(01)92328-3. [DOI] [PubMed] [Google Scholar]
  14. Stevens F. C., Glazer A. N., Smith E. L. The amino acid sequence of wheat germ cytochrome c. J Biol Chem. 1967 Jun 10;242(11):2764–2779. [PubMed] [Google Scholar]
  15. UPHAUS R. A., GROSSWEINER L. I., KATZ J. J., KOPPLE K. D. Fluorescence of tryptophan derivatives in trifluoroacetic acid. Science. 1959 Mar 6;129(3349):641–643. doi: 10.1126/science.129.3349.641. [DOI] [PubMed] [Google Scholar]
  16. Woods K. R., Wang K. T. Separation of dansyl-amino acids by polyamide layer chromatography. Biochim Biophys Acta. 1967 Feb 21;133(2):369–370. doi: 10.1016/0005-2795(67)90078-5. [DOI] [PubMed] [Google Scholar]

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