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. 1970 Apr;117(2):397–403. doi: 10.1042/bj1170397

Studies on the competence-inducing factor of Bacillus subtilis

A Akrigg 1, S R Ayad 1
PMCID: PMC1178873  PMID: 4986873

Abstract

1. Aqueous extracts of competent cells of Bacillus subtilis 168I− were shown to contain a competence-inducing factor. The aqueous extracts were fractionated on DEAE-cellulose columns. 2. Those fractions from DEAE-cellulose columns containing the competence-inducing factor were shown to exhibit a powerful lytic effect on isolated cell walls of B. subtilis 168I−. Little or no lytic activity was exhibited by the other fractions. 3. The kinetics of the lytic enzyme were investigated and found to be first-order. Treatment of cell wall lysates with 1-fluoro-2,4-dinitrobenzene suggested that the enzyme may be identical with N-acetylmuramyl-l-alanine amidase. The amino acid composition of the partially purified enzyme was determined. 4. It is suggested that competence-induction may be dependent on the limited action of the autolytic amidase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akrigg A., Atkinson A., Ayad S. R., Barker G. R. The induction of competence for transformation in Bacillus subtilis. Biochem J. 1967 Aug;104(2):28P–28P. [PMC free article] [PubMed] [Google Scholar]
  2. Akrigg A., Ayad S. R., Barker G. R. The nature of a competence-inducing factor in Bacillus subtilis. Biochem Biophys Res Commun. 1967 Sep 27;28(6):1062–1067. doi: 10.1016/0006-291x(67)90090-3. [DOI] [PubMed] [Google Scholar]
  3. Akrigg A., Ayad S. R., Blamire J. Uptake of DNA by competent bacteria--a possible mechanism. J Theor Biol. 1969 Sep;24(3):266–272. doi: 10.1016/s0022-5193(69)80051-2. [DOI] [PubMed] [Google Scholar]
  4. Akrigg A., Ayad S. R. The competence-inducing factor of Bacillus subtilis. Biochem J. 1969 Mar;112(1):13P–13P. doi: 10.1042/bj1120013p. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bott K. F., Wilson G. A. Development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1967 Sep;94(3):562–570. doi: 10.1128/jb.94.3.562-570.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hirokawa H., Ikeda Y. Genetic Recombination of Transforming Deoxyribonucleic Acid Molecules with the Recipient Genome and Among Themselves in Protoplasts of Bacillus subtilis. J Bacteriol. 1966 Aug;92(2):455–463. doi: 10.1128/jb.92.2.455-463.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pariiskaia A. N., Pukhova E. S. O "faktore kompetentnosti" u Bacillus subtilis. Mikrobiologiia. 1967 May-Jun;36(3):456–463. [PubMed] [Google Scholar]
  8. Tichy P., Landman O. E. Transformation in quasi spheroplasts of Bacillus subtilis. J Bacteriol. 1969 Jan;97(1):42–51. doi: 10.1128/jb.97.1.42-51.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tichý P., Kohoutová M. Transformation of lysozyme spheroplasts of Bacillus subtilis. Folia Microbiol (Praha) 1968;13(4):317–323. doi: 10.1007/BF02909620. [DOI] [PubMed] [Google Scholar]
  10. Wilson G. A., Bott K. F. Nutritional factors influencing the development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1968 Apr;95(4):1439–1449. doi: 10.1128/jb.95.4.1439-1449.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Young F. E. Autolytic enzyme associated with cell walls of Bacillus subtilis. J Biol Chem. 1966 Aug 10;241(15):3462–3467. [PubMed] [Google Scholar]

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