Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1970 May;117(4):773–777. doi: 10.1042/bj1170773

The binding in vitro of colchicine to axoplasmic proteins from chicken sciatic nerve

K A C James 1, L Austin 1
PMCID: PMC1179030  PMID: 5449130

Abstract

1. Axoplasmic proteins were fractionated by means of Sephadex G-200 chromatography followed by isoelectric focusing. Nine groups of proteins were separated. 2. The binding of colchicine to these groups of proteins was examined and it appeared to associate most strongly with one protein group, of pI value 4.9–5.0, which is the major 14C-labelled component of slow-transport protein. 3. Other fractions also bind colchicine. It is not clear whether these are separate proteins or subunits of the major colchicine-binding fraction.

Full text

PDF
773

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bray J. J., Austin L. Axoplasmic transport of 14C proteins at two rates in chicken sciatic nerve. Brain Res. 1969 Jan;12(1):230–233. doi: 10.1016/0006-8993(69)90069-9. [DOI] [PubMed] [Google Scholar]
  2. Dahlström A. Effect of colchicine on transport of amine storage granules in sympathetic nerves of rat. Eur J Pharmacol. 1968 Dec;5(1):111–113. doi: 10.1016/0014-2999(68)90165-9. [DOI] [PubMed] [Google Scholar]
  3. Dahlström A., Häggendal J. Studies on the transport and life-span of amine storage granules in the adrenergic neuron system of the rabbit sciatic nerve. Acta Physiol Scand. 1967 Mar;69(3):153–157. doi: 10.1111/j.1748-1716.1967.tb03506.x. [DOI] [PubMed] [Google Scholar]
  4. GEIGER R. S. THE BEHAVIOR OF ADULT MAMMALIAN BRAIN CELLS IN CULTURE. Int Rev Neurobiol. 1963;5:1–52. doi: 10.1016/s0074-7742(08)60593-0. [DOI] [PubMed] [Google Scholar]
  5. James K. A., Bray J. J., Morgan I. G., Austin L. The effect of colchicine on the transport of axonal protein in the chicken. Biochem J. 1970 May;117(4):767–771. doi: 10.1042/bj1170767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kidwai A. M., Ochs S. Components of fast and slow phases of axoplasmic flow. J Neurochem. 1969 Jul;16(7):1105–1112. doi: 10.1111/j.1471-4159.1969.tb05955.x. [DOI] [PubMed] [Google Scholar]
  7. Kreutzberg G. W. Neuronal dynamics and axonal flow. IV. Blockage of intra-axonal enzyme transport by colchicine. Proc Natl Acad Sci U S A. 1969 Mar;62(3):722–728. doi: 10.1073/pnas.62.3.722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Livett B. G., Geffen L. B., Austin L. Proximo-distal transport of [14C]noradrenaline and protein in sympathetic nerves. J Neurochem. 1968 Sep;15(9):931–939. doi: 10.1111/j.1471-4159.1968.tb11635.x. [DOI] [PubMed] [Google Scholar]
  9. McEwen B. S., Grafstein B. Fast and slow components in axonal transport of protein. J Cell Biol. 1968 Sep;38(3):494–508. doi: 10.1083/jcb.38.3.494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rosenbaum J. L., Carlson K. Cilia regeneration in Tetrahymena and its inhibition by colchicine. J Cell Biol. 1969 Feb;40(2):415–425. doi: 10.1083/jcb.40.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tilney L. G. Studies on the microtubules in heliozoa. IV. The effect of colchicine on the formation and maintenance of the axopodia and the redevelopment of pattern in Actinosphaerium nucleofilum (Barrett). J Cell Sci. 1968 Dec;3(4):549–562. doi: 10.1242/jcs.3.4.549. [DOI] [PubMed] [Google Scholar]
  12. Weisenberg R. C., Borisy G. G., Taylor E. W. The colchicine-binding protein of mammalian brain and its relation to microtubules. Biochemistry. 1968 Dec;7(12):4466–4479. doi: 10.1021/bi00852a043. [DOI] [PubMed] [Google Scholar]
  13. Wisniewski H., Shelanski M. L., Terry R. D. Effects of mitotic spindle inhibitors on neurotubules and neurofilaments in anterior horn cells. J Cell Biol. 1968 Jul;38(1):224–229. doi: 10.1083/jcb.38.1.224. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES