Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1970 Jun;118(1):89–92. doi: 10.1042/bj1180089

The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-d-glucosamine by Escherichia coli

R J White 1,*
PMCID: PMC1179084  PMID: 4919472

Abstract

The properties of an N-acetyl-d-glucosamine-transport system have been studied by following the intracellular accumulation of methyl 2-acetamido-2-deoxy-α-d-[1-14C]glucoside by Escherichia coli. The same analogue was used to assay phosphoenolpyruvate phosphotransferase activity of toluene-treated cells. Transport and phosphorylation are induced by growth on d-glucosamine or N-acetyl-d-glucosamine. Mutants resistant to N-iodoacetyl-d-glucosamine are defective in the uptake and phosphorylation of the labelled glycoside.

Full text

PDF
90

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COHEN G. N., MONOD J. Bacterial permeases. Bacteriol Rev. 1957 Sep;21(3):169–194. doi: 10.1128/br.21.3.169-194.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. EGAN J. B., MORSE M. L. CARBOHYDRATE TRANSPORT IN STAPHYLOCOCCUS AUREUS I. GENETIC AND BIOCHEMICAL ANALYSIS OF A PLEIOTROPIC TRANSPORT MUTANT. Biochim Biophys Acta. 1965 Feb 15;97:310–319. doi: 10.1016/0304-4165(65)90096-6. [DOI] [PubMed] [Google Scholar]
  3. HAGIHIRA H., WILSON T. H., LIN E. C. STUDIES ON THE GLUCOSE-TRANSPORT SYSTEM IN ESCHERICHIA COLI WITH ALPHA-METHYLGLUCOSIDE AS SUBSTRATE. Biochim Biophys Acta. 1963 Nov 15;78:505–515. doi: 10.1016/0006-3002(63)90912-0. [DOI] [PubMed] [Google Scholar]
  4. HOFFEE P., ENGLESBERG E. Effect of metabolic activity on the glucose permease of bacterial cells. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1759–1765. doi: 10.1073/pnas.48.10.1759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HOFFEE P., ENGLESBERG E., LAMY F. THE GLUCOSE PERMEASE SYSTEM IN BACTERIA. Biochim Biophys Acta. 1964 Mar 30;79:337–350. [PubMed] [Google Scholar]
  6. KUNDIG W., GHOSH S., ROSEMAN S. PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM. Proc Natl Acad Sci U S A. 1964 Oct;52:1067–1074. doi: 10.1073/pnas.52.4.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaback H. R. The role of the phosphoenolpyruvate-phosphotransferase system in the transport of sugars by isolated membrane preparations of Escherichia coli. J Biol Chem. 1968 Jul 10;243(13):3711–3724. [PubMed] [Google Scholar]
  8. Kennedy E. P., Scarborough G. A. Mechanism of hydrolysis of O-nitrophenyl-beta-galactoside in Staphylococcus aureus and its significance for theories of sugar transport. Proc Natl Acad Sci U S A. 1967 Jul;58(1):225–228. doi: 10.1073/pnas.58.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MATSUSHIMA Y., MIYAZAKI T., PARK J. T. SEPARATION OF METHYL N-ACETYL-D-GLUCOSAMINIDE ANOMERS. J Biochem. 1963 Jul;54:109–110. doi: 10.1093/oxfordjournals.jbchem.a127740. [DOI] [PubMed] [Google Scholar]
  10. OSBORN M. J., McLELLAN W. L., Jr, HORECKER B. L. Galactose transport in Escherichia coli. III. The effect of 2,4-dinitrophenol on entry and accumulation. J Biol Chem. 1961 Oct;236:2585–2589. [PubMed] [Google Scholar]
  11. Pavlasova E., Harold F. M. Energy coupling in the transport of beta-galactosides by Escherichia coli: effect of proton conductors. J Bacteriol. 1969 Apr;98(1):198–204. doi: 10.1128/jb.98.1.198-204.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Simoni R. D., Levinthal M., Kundig F. D., Kundig W., Anderson B., Hartman P. E., Roseman S. Genetic evidence for the role of a bacterial phosphotransferase system in sugar transport. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1963–1970. doi: 10.1073/pnas.58.5.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. White R. J. Control of amino sugar metabolism in Escherichia coli and isolation of mutants unable to degrade amino sugars. Biochem J. 1968 Feb;106(4):847–858. doi: 10.1042/bj1060847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. White R. J., Kent P. W. An examination of the inhibitory effects of N-iodoacetylglucosamine on Escherichia coli and isolation of resistant mutants. Biochem J. 1970 Jun;118(1):81–87. doi: 10.1042/bj1180081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. White R. J., Pasternak C. A. The purification and properties of N-acetylglucosamine 6-phosphate deacetylase from Escherichia coli. Biochem J. 1967 Oct;105(1):121–125. doi: 10.1042/bj1050121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Winkler H. H., Wilson T. H. The role of energy coupling in the transport of beta-galactosides by Escherichia coli. J Biol Chem. 1966 May 25;241(10):2200–2211. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES