Abstract
Solutions of crystalline β-lactamase I and β-lactamase II, prepared by Kuwabara (1970), were examined in the ultracentrifuge and their sedimentation coefficients, diffusion coefficients, molecular weights and heterogeneity determined. Each sample was shown to consist of a major component comprising at least 97% of the material and a minor component of much higher molecular weight. The molecular weights of the major components were 27800 for β-lactamase I and 35600 for β-lactamase II. Emphasis is placed on a straightforward practical way of analysing the sedimentation-equilibrium results on mixtures of two macromolecular components rather than on a strict theoretical solution. Appendices describe the theory of systems at both chemical and sedimentation equilibrium and the procedure for calculating the combined distribution of two components.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adams E. T., Jr Osmotic pressure of associating systems. I. Basic theory. Biochemistry. 1965 Aug;4(8):1655–1659. doi: 10.1021/bi00884a030. [DOI] [PubMed] [Google Scholar]
- Adams E. T., Jr Sedimentation equilibrium in reacting systems. V. The application of sigma-ci-Mi2 to the analysis. Biochemistry. 1967 Jun;6(6):1864–1871. doi: 10.1021/bi00858a038. [DOI] [PubMed] [Google Scholar]
- GROPPER L. OPTICAL ALIGNMENT PROCEDURE FOR THE ANALYTICAL ULTRACENTRIFUGE. Anal Biochem. 1964 Apr;7:401–428. doi: 10.1016/0003-2697(64)90152-6. [DOI] [PubMed] [Google Scholar]
- HALL J. R., OGSTON A. G. Sedimentation and diffusion of samples of penicillinase. Biochem J. 1956 Mar;62(3):401–403. [PubMed] [Google Scholar]
- Kuwabara S. Purification and properties of two extracellular beta-lactamases from Bacillus cereus 569-H. Biochem J. 1970 Jul;118(3):457–465. doi: 10.1042/bj1180457. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McMEEKIN T. L., MARSHALL K. Specific volumes of proteins and the relationship to their amino acid contents. Science. 1952 Aug 8;116(3006):142–143. doi: 10.1126/science.116.3006.142. [DOI] [PubMed] [Google Scholar]
- Richards E. G., Teller D. C., Schachman H. K. Ultracentrifuge studies with Rayleigh interference optics. II. Low-speed sedimentation equilibrium of homogeneous systems. Biochemistry. 1968 Mar;7(3):1054–1076. doi: 10.1021/bi00843a026. [DOI] [PubMed] [Google Scholar]