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. 1970 Jul;118(4):557–561. doi: 10.1042/bj1180557

Isolation of bacilysin and a new amino acid from culture filtrates of Bacillus subtilis

J E Walker 1,*, E P Abraham 1
PMCID: PMC1179251  PMID: 4991475

Abstract

1. Bacilysin, a labile dipeptide antibiotic that lyses growing staphylococci, was isolated from culture fluids of Bacillus subtilis by a process giving higher yields than those previously obtained. 2. The process involves adsorption on a cation-exchange resin and elution with aqueous trimethylamine, separation from neutral amino acids and glutamic acid by chromatography on DEAE-Sephadex at pH8.7 and separation from other neutral peptides by chromatography in aqueous propan-2-ol on Sephadex G-25. 3. A new amino acid, which is chemically related to bacilysin, was isolated from the fraction containing neutral amino acids. 4. Two substances that yield alanine on hydrolysis, in addition to bacilysin, were obtained from the neutral peptide fraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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