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. 1970 Aug;118(5):713–718. doi: 10.1042/bj1180713

Purification and amino acid sequence of melanocyte-stimulating hormone from the dogfish Squalus acanthias

P J Lowry 1,*, A Chadwick 1
PMCID: PMC1179279  PMID: 5476715

Abstract

A melanocyte-stimulating hormone (MSH) was isolated by gel filtration and ion-exchange chromatography from extracts of the pituitary glands of dogfish. Sequence studies were carried out on the hormone and its enzymically and chemically cleaved fragments. The sequence of the hormone, Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Met, shows that ten of its 11 residues are the same as ten of the 13 residues of mammalian α-MSH. About half of its molecules have the carboxyl group at the C-terminus free and about half are amidated; about a fifth have an extra tyrosine residue on the N-terminus, thereby making 11 residues the same as in mammalian α-MSH. Unlike the mammalian hormone, however, none of it was found to be N-acetylated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURGERS A. C. Melanophore-stimulating hormones in vertebrates. Ann N Y Acad Sci. 1963 Feb 15;100:669–677. doi: 10.1111/j.1749-6632.1963.tb42923.x. [DOI] [PubMed] [Google Scholar]
  2. CRAIG L. C., ANSEVIN A. DIALYSIS STUDIES. VI. EXPERIMENTS WITH AMINO ACIDS. Biochemistry. 1963 Nov-Dec;2:1268–1271. doi: 10.1021/bi00906a017. [DOI] [PubMed] [Google Scholar]
  3. Edelhoch H. Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry. 1967 Jul;6(7):1948–1954. doi: 10.1021/bi00859a010. [DOI] [PubMed] [Google Scholar]
  4. Gros C., Labouesse B. Study of the dansylation reaction of amino acids, peptides and proteins. Eur J Biochem. 1969 Feb;7(4):463–470. doi: 10.1111/j.1432-1033.1969.tb19632.x. [DOI] [PubMed] [Google Scholar]
  5. KOSTKA V., CARPENTER F. H. INHIBITION OF CHYMOTRYPSIN ACTIVITY IN CRYSTALLINE TRYPSIN PREPARATIONS. J Biol Chem. 1964 Jun;239:1799–1803. [PubMed] [Google Scholar]
  6. LO T. B., DIXON J. S., LI C. H. Isolation of methionine sulfoxide analogue of alpha-melanocyte-stimulating hormone from bovine pituitary glands. Biochim Biophys Acta. 1961 Nov 11;53:584–586. doi: 10.1016/0006-3002(61)90221-9. [DOI] [PubMed] [Google Scholar]
  7. Lowry P. J., Chadwick A. Interrelations of some pituitary hormones. Nature. 1970 Apr 18;226(5242):219–222. doi: 10.1038/226219a0. [DOI] [PubMed] [Google Scholar]
  8. Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
  9. PIEZ K. A., MORRIS L. A modified procedure for the automatic analysis of amino acids. Anal Biochem. 1960 Nov;1:187–201. doi: 10.1016/0003-2697(60)90045-2. [DOI] [PubMed] [Google Scholar]
  10. Pickering B. T., Heller H. Oxytocin as a neurophyophysial hormone in the holocephalian elasmobranch fish, Hydrolagus collei. J Endocrinol. 1969 Dec;45(4):597–606. doi: 10.1677/joe.0.0450597. [DOI] [PubMed] [Google Scholar]
  11. SMITH I. Colour reactions on paper chromatograms by a dipping technique. Nature. 1953 Jan 3;171(4340):43–44. doi: 10.1038/171043a0. [DOI] [PubMed] [Google Scholar]
  12. STEERS E., Jr, CRAVEN G. R., ANFINSEN C. B., BETHUNE J. L. EVIDENCE FOR NONIDENTICAL CHAINS IN THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12. J Biol Chem. 1965 Jun;240:2478–2484. [PubMed] [Google Scholar]
  13. Woods K. R., Wang K. T. Separation of dansyl-amino acids by polyamide layer chromatography. Biochim Biophys Acta. 1967 Feb 21;133(2):369–370. doi: 10.1016/0005-2795(67)90078-5. [DOI] [PubMed] [Google Scholar]

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