Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1970 Sep;119(3):535–539. doi: 10.1042/bj1190535

The amino acid sequence of Helianthus annuus L (sunflower) cytochrome c deduced from chymotrypic peptides

J A M Ramshaw 1, E W Thompson 1, D Boulter 1
PMCID: PMC1179384  PMID: 5500313

Abstract

Peptides derived from digestion of 1 μmol of sunflower cytochrome c with chymotrypsin were separated by paper electrophoresis. The sequences of these peptides were determined by using the dansyl–Edman method (Gray & Hartley, 1963) and confirmed by analysis of their amino acid composition. Comparison of the set of peptides with the chymotryptic peptides of mung-bean (Thompson, Laycock, Ramshaw & Boulter, 1970) and wheat germ (Stevens, Glazer & Smith, 1967) cytochrome c shows a clear homology. The complete sequence of sunflower cytochrome c was established by alignment of the sunflower peptides with the sequences of mung bean cytochrome c and wheat germ cytochrome c.

Full text

PDF
539

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DeLange R. J., Glazer A. N., Smith E. L. Presence and location of an unusual amino acid, epsilon-N-trimethyllysine, in cytochrome c of wheat germ and Neurospora. J Biol Chem. 1969 Mar 10;244(5):1385–1388. [PubMed] [Google Scholar]
  2. GRAY W. R., HARTLEY B. S. THE STRUCTURE OF A CHYMOTRYPTIC PEPTIDE FROM PSEUDOMONAS CYTOCHROME C-551. Biochem J. 1963 Nov;89:379–380. doi: 10.1042/bj0890379. [DOI] [PubMed] [Google Scholar]
  3. MARGOLIASH E., KIMMEL J. R., HILL R. L., SCHMIDT W. R. Amino acid composition of horse heart cytochrome c. J Biol Chem. 1962 Jul;237:2148–2150. [PubMed] [Google Scholar]
  4. Nolan C., Margoliash E. Primary structure of the cytochrome c from the great grey kangaroo, Macropus canguru. J Biol Chem. 1966 Mar 10;241(5):1049–1059. [PubMed] [Google Scholar]
  5. Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
  6. Schmer G., Kreil G. Micro method for detection of formyl and acetyl groups in proteins. Anal Biochem. 1969 May;29(2):186–192. doi: 10.1016/0003-2697(69)90301-7. [DOI] [PubMed] [Google Scholar]
  7. Stevens F. C., Glazer A. N., Smith E. L. The amino acid sequence of wheat germ cytochrome c. J Biol Chem. 1967 Jun 10;242(11):2764–2779. [PubMed] [Google Scholar]
  8. Thompson E. W., Laycock M. V., Ramshaw J. A., Boulter D. The amino acid sequence of Phaseolus aureua L. (mung-bean) cytochrome c. Biochem J. 1970 Mar;117(1):183–192. doi: 10.1042/bj1170183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. UPHAUS R. A., GROSSWEINER L. I., KATZ J. J., KOPPLE K. D. Fluorescence of tryptophan derivatives in trifluoroacetic acid. Science. 1959 Mar 6;129(3349):641–643. doi: 10.1126/science.129.3349.641. [DOI] [PubMed] [Google Scholar]
  10. Woods K. R., Wang K. T. Separation of dansyl-amino acids by polyamide layer chromatography. Biochim Biophys Acta. 1967 Feb 21;133(2):369–370. doi: 10.1016/0005-2795(67)90078-5. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES