Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1991 Dec;444:303–315. doi: 10.1113/jphysiol.1991.sp018879

Renovascular effects of neuropeptide-Y in the split hydronephrotic rat kidney: non-uniform pattern of vascular reactivity.

M S Dietrich 1, M Fretschner 1, R Nobiling 1, P B Persson 1, M Steinhausen 1
PMCID: PMC1179934  PMID: 1822552

Abstract

1. The renovascular effects of neuropeptide-Y (NPY) were examined in the split hydronephrotic rat kidney. 2. Systemic infusion of low non-pressor doses of NPY (0.2 micrograms kg-1 up to 5.0 micrograms kg-1) produced a non-uniform pattern of vascular reactivity. In general, a significant constriction of the proximal and distal arcuate artery was seen at all doses. No constriction was seen at the interlobular artery or the larger part of the afferent arteriole. These segments initially dilated during the lower dose infusions. The very distal part of the afferent arteriole adjacent to the glomerulus and the proximal efferent arteriole responded in a similar way to the arcuate arteries. 3. NPY, locally applied into the tissue bath at concentrations of 1 nmol l-1 up to 25 nmol l-1, produced non-uniform vascular reactions similar to those of intravenously infused NPY. At the considerably higher local dosage of 1.14 mumol l-1, all vascular segments revealed vasoconstriction. 4. NPY application did not attenuate effects of acetylcholine. This observation suggests that the mechanism of NPY-induced vasoconstriction does not rely upon antagonism of endothelium-derived vasodilatation. 5. The pattern of vascular reactivity to NPY was substantially different from that known for the vasoconstrictors noradrenaline and angiotensin II in our preparation.

Full text

PDF
312

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Budai D., Vu H. Q., Duckles S. P. Endothelium removal does not affect potentiation by neuropeptide Y in rabbit ear artery. Eur J Pharmacol. 1989 Sep 1;168(1):97–100. doi: 10.1016/0014-2999(89)90638-9. [DOI] [PubMed] [Google Scholar]
  2. Daly R. N., Hieble J. P. Neuropeptide Y modulates adrenergic neurotransmission by an endothelium dependent mechanism. Eur J Pharmacol. 1987 Jun 26;138(3):445–446. doi: 10.1016/0014-2999(87)90486-9. [DOI] [PubMed] [Google Scholar]
  3. Echtenkamp S. F., Dandridge P. F. Renal actions of neuropeptide Y in the primate. Am J Physiol. 1989 Apr;256(4 Pt 2):F524–F531. doi: 10.1152/ajprenal.1989.256.4.F524. [DOI] [PubMed] [Google Scholar]
  4. Fallgren B., Ekblad E., Edvinsson L. Co-existence of neuropeptides and differential inhibition of vasodilator responses by neuropeptide Y in guinea pig uterine arteries. Neurosci Lett. 1989 May 22;100(1-3):71–76. doi: 10.1016/0304-3940(89)90662-9. [DOI] [PubMed] [Google Scholar]
  5. Furchgott R. F. Role of endothelium in responses of vascular smooth muscle. Circ Res. 1983 Nov;53(5):557–573. doi: 10.1161/01.res.53.5.557. [DOI] [PubMed] [Google Scholar]
  6. Gaehtgens P. Flow of blood through narrow capillaries: rheological mechanisms determining capillary hematocrit and apparent viscosity. Biorheology. 1980;17(1-2):183–189. doi: 10.3233/bir-1980-171-220. [DOI] [PubMed] [Google Scholar]
  7. Gustafsson H., Nilsson H. Endothelium-independent potentiation by neuropeptide Y of vasoconstrictor responses in isolated arteries from rat and rabbit. Acta Physiol Scand. 1990 Apr;138(4):503–507. doi: 10.1111/j.1748-1716.1990.tb08878.x. [DOI] [PubMed] [Google Scholar]
  8. Hackenthal E., Aktories K., Jakobs K. H., Lang R. E. Neuropeptide Y inhibits renin release by a pertussis toxin-sensitive mechanism. Am J Physiol. 1987 Mar;252(3 Pt 2):F543–F550. doi: 10.1152/ajprenal.1987.252.3.F543. [DOI] [PubMed] [Google Scholar]
  9. Hieble J. P., Duesler J. G., Jr, Daly R. N. Effects of neuropeptide Y on the response of isolated blood vessels to norepinephrine and sympathetic field stimulation. J Pharmacol Exp Ther. 1989 Aug;250(2):523–528. [PubMed] [Google Scholar]
  10. Intaglietta M., Silverman N. R., Tompkins W. R. Capillary flow velocity measurements in vivo and in situ by television methods. Microvasc Res. 1975 Sep;10(2):165–179. doi: 10.1016/0026-2862(75)90004-7. [DOI] [PubMed] [Google Scholar]
  11. Intaglietta M., Tompkins W. R. Microvascular measurements by video image shearing and splitting. Microvasc Res. 1973 May;5(3):309–312. doi: 10.1016/0026-2862(73)90042-3. [DOI] [PubMed] [Google Scholar]
  12. Kwan Y. W., Wadsworth R. M., Kane K. A. Effects of neuropeptide Y and calcitonin gene-related peptide on sheep coronary artery rings under oxygenated, hypoxic and simulated myocardial ischaemic conditions. Br J Pharmacol. 1990 Apr;99(4):774–778. doi: 10.1111/j.1476-5381.1990.tb13005.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mejia J. A., Pernow J., von Holst H., Rudehill A., Lundberg J. M. Effects of neuropeptide Y, calcitonin gene-related peptide, substance P, and capsaicin on cerebral arteries in man and animals. J Neurosurg. 1988 Dec;69(6):913–918. doi: 10.3171/jns.1988.69.6.0913. [DOI] [PubMed] [Google Scholar]
  14. Minson R., McRitchie R., Chalmers J. Effects of neuropeptide Y on the renal, mesenteric and hindlimb vascular beds of the conscious rabbit. J Auton Nerv Syst. 1989 Jul;27(2):139–146. doi: 10.1016/0165-1838(89)90095-7. [DOI] [PubMed] [Google Scholar]
  15. Nobiling R., Bührle C. P., Hackenthal E., Helmchen U., Steinhausen M., Whalley A., Taugner R. Ultrastructure, renin status, contractile and electrophysiological properties of the afferent glomerular arteriole in the rat hydronephrotic kidney. Virchows Arch A Pathol Anat Histopathol. 1986;410(1):31–42. doi: 10.1007/BF00710903. [DOI] [PubMed] [Google Scholar]
  16. Nobiling R., Gabel M., Persson P. B., Dietrich M. S., Bührle C. P. Differential effect of neuropeptide-Y on membrane potential of cells in renal arterioles of the hydronephrotic mouse. J Physiol. 1991 Dec;444:317–327. doi: 10.1113/jphysiol.1991.sp018880. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pernow J. Actions of constrictor (NPY and endothelin) and dilator (substance P, CGRP and VIP) peptides on pig splenic and human skeletal muscle arteries: involvement of the endothelium. Br J Pharmacol. 1989 Jul;97(3):983–989. doi: 10.1111/j.1476-5381.1989.tb12040.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pernow J., Lundberg J. M. Release and vasoconstrictor effects of neuropeptide Y in relation to non-adrenergic sympathetic control of renal blood flow in the pig. Acta Physiol Scand. 1989 Aug;136(4):507–517. doi: 10.1111/j.1748-1716.1989.tb08696.x. [DOI] [PubMed] [Google Scholar]
  19. Persson P. B., Ehmke H., Nafz B., Lang R., Hackenthal E., Nobiling R., Dietrich M. S., Kirchheim H. R. Effects of neuropeptide-Y on renal function and its interaction with sympathetic stimulation in conscious dogs. J Physiol. 1991 Dec;444:289–302. doi: 10.1113/jphysiol.1991.sp018878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Steinhausen M., Blum M., Fleming J. T., Holz F. G., Parekh N., Wiegman D. L. Visualization of renal autoregulation in the split hydronephrotic kidney of rats. Kidney Int. 1989 May;35(5):1151–1160. doi: 10.1038/ki.1989.104. [DOI] [PubMed] [Google Scholar]
  21. Steinhausen M., Endlich K., Wiegman D. L. Glomerular blood flow. Kidney Int. 1990 Nov;38(5):769–784. doi: 10.1038/ki.1990.271. [DOI] [PubMed] [Google Scholar]
  22. Steinhausen M., Snoei H., Parekh N., Baker R., Johnson P. C. Hydronephrosis: a new method to visualize vas afferens, efferens, and glomerular network. Kidney Int. 1983 Jun;23(6):794–806. doi: 10.1038/ki.1983.98. [DOI] [PubMed] [Google Scholar]
  23. Taugner R., Hackenthal E., Nobiling R., Harlacher M., Reb G. The distribution of renin in the different segments of the renal arterial tree: immunocytochemical investigation in the mouse kidney. Histochemistry. 1981;73(1):75–88. doi: 10.1007/BF00493135. [DOI] [PubMed] [Google Scholar]
  24. Wayland H., Johnson P. C. Erythrocyte velocity measurement in microvessels by a two-slit photometric method. J Appl Physiol. 1967 Feb;22(2):333–337. doi: 10.1152/jappl.1967.22.2.333. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES