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. 1990 Dec;431:495–503. doi: 10.1113/jphysiol.1990.sp018342

Prolonged inhibition of cardiac vagal action following sympathetic stimulation and galanin in anaesthetized cats.

M Revington 1, E K Potter 1, D I McCloskey 1
PMCID: PMC1181786  PMID: 1712845

Abstract

1. Stimulation of the right cardiac sympathetic nerve for 3 min at 16 Hz in the presence of effective beta-adrenoceptor blockade evoked prolonged attention of cardiac vagal action in the cat: 40.8 +/- 5.4% maximum inhibition of cardiac vagal action on prolonging pulse interval, with half-time to recovery of 8.3 +/- 1.4 min. 2. Intravenous injection of galanin (1.6-3.1 nmol/kg) evoked prolonged attenuation of cardiac vagal action: 40.9 +/- 8.2% maximum inhibition with a half-time to recovery of 13.6 +/- 2.6 min. This effect of galanin was not significantly different from the action of sympathetic nerve stimulation. A slight depressor response (-14.4 +/- 1.9 mmHg) was seen in nine of sixteen cats. 3. Intravenous injection of neuropeptide Y (NPY) (2.8-6.3 nmol/kg) evoked slight attenuation of cardiac vagal action: 11.9 +/- 4.5% maximum inhibition of cardiac vagal action on pulse interval, with a half-time to recovery of 4.1 +/- 1.7 min. Blood pressure increased by 68.6 +/- 5.7 mmHg. 4. Following administration of guanethidine (1 mg/kg I.V.) the inhibitory effect of sympathetic nerve stimulation on cardiac vagal action was significantly reduced (P less than 0.001). The responses to exogenous NPY and galanin on vagal action were unchanged after guanethidine. 5. The prolonged attenuation of cardiac vagal action can be mimicked by exogenous galanin in the cat but not by exogenous NPY.

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Selected References

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  1. Allen J. M., Bircham P. M., Edwards A. V., Tatemoto K., Bloom S. R. Neuropeptide Y (NPY) reduces myocardial perfusion and inhibits the force of contraction of the isolated perfused rabbit heart. Regul Pept. 1983 Jul;6(3):247–253. doi: 10.1016/0167-0115(83)90143-x. [DOI] [PubMed] [Google Scholar]
  2. Darvesh S., Nance D. M., Hopkins D. A., Armour J. A. Distribution of neuropeptide-like immunoreactivity in intact and chronically decentralized middle cervical and stellate ganglia of dogs. J Auton Nerv Syst. 1987 Dec;21(2-3):167–180. doi: 10.1016/0165-1838(87)90019-1. [DOI] [PubMed] [Google Scholar]
  3. Dunning B. E., Taborsky G. J., Jr Galanin release during pancreatic nerve stimulation is sufficient to influence islet function. Am J Physiol. 1989 Jan;256(1 Pt 1):E191–E198. doi: 10.1152/ajpendo.1989.256.1.E191. [DOI] [PubMed] [Google Scholar]
  4. Fisone G., Wu C. F., Consolo S., Nordström O., Brynne N., Bartfai T., Melander T., Hökfelt T. Galanin inhibits acetylcholine release in the ventral hippocampus of the rat: histochemical, autoradiographic, in vivo, and in vitro studies. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7339–7343. doi: 10.1073/pnas.84.20.7339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gardner T. D., Potter E. K. Dependence of non-adrenergic inhibition of cardiac vagal action on peak frequency of sympathetic stimulation in the dog. J Physiol. 1988 Nov;405:115–122. doi: 10.1113/jphysiol.1988.sp017324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gu J., Polak J. M., Adrian T. E., Allen J. M., Tatemoto K., Bloom S. R. Neuropeptide tyrosine (NPY)--a major cardiac neuropeptide. Lancet. 1983 May 7;1(8332):1008–1010. doi: 10.1016/s0140-6736(83)92642-9. [DOI] [PubMed] [Google Scholar]
  7. Katona P. G., Poitras J. W., Barnett G. O., Terry B. S. Cardiac vagal efferent activity and heart period in the carotid sinus reflex. Am J Physiol. 1970 Apr;218(4):1030–1037. doi: 10.1152/ajplegacy.1970.218.4.1030. [DOI] [PubMed] [Google Scholar]
  8. Kilborn M. J., Potter E. K., McCloskey D. I. Neuromodulation of the cardiac vagus: comparison of neuropeptide Y and related peptides. Regul Pept. 1985 Oct;12(2):155–161. doi: 10.1016/0167-0115(85)90196-x. [DOI] [PubMed] [Google Scholar]
  9. Kummer W. Galanin- and neuropeptide Y-like immunoreactivities coexist in paravertebral sympathetic neurones of the cat. Neurosci Lett. 1987 Jul 22;78(2):127–131. doi: 10.1016/0304-3940(87)90620-3. [DOI] [PubMed] [Google Scholar]
  10. Lundberg J. M., Anggård A., Theodorsson-Norheim E., Pernow J. Guanethidine-sensitive release of neuropeptide Y-like immunoreactivity in the cat spleen by sympathetic nerve stimulation. Neurosci Lett. 1984 Nov 23;52(1-2):175–180. doi: 10.1016/0304-3940(84)90370-7. [DOI] [PubMed] [Google Scholar]
  11. Lundberg J. M., Tatemoto K. Pancreatic polypeptide family (APP, BPP, NPY and PYY) in relation to sympathetic vasoconstriction resistant to alpha-adrenoceptor blockade. Acta Physiol Scand. 1982 Dec;116(4):393–402. doi: 10.1111/j.1748-1716.1982.tb07157.x. [DOI] [PubMed] [Google Scholar]
  12. Mabe Y., Pérez R., Tatemoto K., Huidobro-Toro J. P. Chemical sympathectomy reveals pre- and postsynaptic effects of neuropeptide Y (NPY) in the cardiovascular system. Experientia. 1987 Sep 15;43(9):1018–1020. doi: 10.1007/BF01952225. [DOI] [PubMed] [Google Scholar]
  13. Maggi C. A., Santicioli P., Patacchini R., Turini D., Barbanti G., Beneforti P., Giuliani S., Meli A. Galanin: a potent modulator of excitatory neurotransmission in the human urinary bladder. Eur J Pharmacol. 1987 Nov 3;143(1):135–137. doi: 10.1016/0014-2999(87)90744-8. [DOI] [PubMed] [Google Scholar]
  14. Maturi M. F., Greene R., Speir E., Burrus C., Dorsey L. M., Markle D. R., Maxwell M., Schmidt W., Goldstein S. R., Patterson R. E. Neuropeptide-Y. A peptide found in human coronary arteries constricts primarily small coronary arteries to produce myocardial ischemia in dogs. J Clin Invest. 1989 Apr;83(4):1217–1224. doi: 10.1172/JCI114004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nordström O., Melander T., Hökfelt T., Bartfai T., Goldstein M. Evidence for an inhibitory effect of the peptide galanin on dopamine release from the rat median eminence. Neurosci Lett. 1987 Jan 2;73(1):21–26. doi: 10.1016/0304-3940(87)90024-3. [DOI] [PubMed] [Google Scholar]
  16. Ohhashi T., Jacobowitz D. M. Galanin potentiates electrical stimulation and exogenous norepinephrine-induced contractions in the rat vas deferens. Regul Pept. 1985 Oct;12(2):163–171. doi: 10.1016/0167-0115(85)90197-1. [DOI] [PubMed] [Google Scholar]
  17. Potter E. K. Guanethidine blocks neuropeptide-Y-like inhibitory action of sympathetic nerves on cardiac vagus. J Auton Nerv Syst. 1987 Nov;21(1):87–90. doi: 10.1016/0165-1838(87)90095-6. [DOI] [PubMed] [Google Scholar]
  18. Potter E. K. Neuropeptide Y as an autonomic neurotransmitter. Pharmacol Ther. 1988;37(2):251–273. doi: 10.1016/0163-7258(88)90028-9. [DOI] [PubMed] [Google Scholar]
  19. Potter E. K. Prolonged non-adrenergic inhibition of cardiac vagal action following sympathetic stimulation: neuromodulation by neuropeptide Y? Neurosci Lett. 1985 Mar 15;54(2-3):117–121. doi: 10.1016/s0304-3940(85)80065-3. [DOI] [PubMed] [Google Scholar]
  20. Potter E. Presynaptic inhibition of cardiac vagal postganglionic nerves by neuropeptide Y. Neurosci Lett. 1987 Dec 16;83(1-2):101–106. doi: 10.1016/0304-3940(87)90223-0. [DOI] [PubMed] [Google Scholar]
  21. Rudehill A., Sollevi A., Franco-Cereceda A., Lundberg J. M. Neuropeptide Y (NPY) and the pig heart: release and coronary vasoconstrictor effects. Peptides. 1986 Sep-Oct;7(5):821–826. doi: 10.1016/0196-9781(86)90101-4. [DOI] [PubMed] [Google Scholar]
  22. Rökaeus A., Melander T., Hökfelt T., Lundberg J. M., Tatemoto K., Carlquist M., Mutt V. A galanin-like peptide in the central nervous system and intestine of the rat. Neurosci Lett. 1984 Jun 15;47(2):161–166. doi: 10.1016/0304-3940(84)90423-3. [DOI] [PubMed] [Google Scholar]
  23. Tatemoto K., Rökaeus A., Jörnvall H., McDonald T. J., Mutt V. Galanin - a novel biologically active peptide from porcine intestine. FEBS Lett. 1983 Nov 28;164(1):124–128. doi: 10.1016/0014-5793(83)80033-7. [DOI] [PubMed] [Google Scholar]
  24. Yau W. M., Dorsett J. A., Youther M. L. Evidence for galanin as an inhibitory neuropeptide on myenteric cholinergic neurons in the guinea pig small intestine. Neurosci Lett. 1986 Dec 23;72(3):305–308. doi: 10.1016/0304-3940(86)90531-8. [DOI] [PubMed] [Google Scholar]

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