Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1977 Dec 1;167(3):843–846. doi: 10.1042/bj1670843

The mechanism of action of primary alkylsulphohydrolase and arylsulphohydrolase from a detergent-degrading micro-organism.

J M Cloves, K S Dodgson, D E Games, D J Shaw, G F White
PMCID: PMC1183735  PMID: 603638

Abstract

Previous studies have shown that secondary alkylsulphohydrolases from certain detergent-degrading micro-organisms are unusual esterases in that they catalyse fission of the C-O bond of the alkyl sulphate ester linkage. The position of bond fission catalysed by a primary alkylsulphatase and an arylsulphohydrolase present in Pseudomonas C12B has now been investigated. The primary alkylsulphatase behaved like the secondary alkylsulphohydrolases in cleaving the C-O bond of potassium heptan-1-yl sulphate. In contrast, the arylsulphohydrolase, in common with other similar enzymes previously studied, catalysed the fission of the O-S bond of potassium p-nitrophenyl sulphate.

Full text

PDF
844

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartholomew B., Dodgson K. S., Matcham G. W., Shaw D. J., White G. F. A novel mechanism of enzymic ester hydrolysis. Inversion of configuration and carbon-oxygen bond cleavage by secondary alkylsulphohydrolases from detergent-degrading micro-organisms. Biochem J. 1977 Sep 1;165(3):575–580. doi: 10.1042/bj1650575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DODGSON K. S., SPENCER B. Assay of sulfatases. Methods Biochem Anal. 1957;4:211–255. doi: 10.1002/9780470110201.ch6. [DOI] [PubMed] [Google Scholar]
  3. Dodgson K. S., Fitzgerald J. W., Payne W. J. Chemically defined inducers of alkylsulphatases present in Pseudomonas C12B. Biochem J. 1974 Jan;138(1):53–62. doi: 10.1042/bj1380053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fitzgerald J. W., Payne W. J. Induction in a Pseudomonas species of sulphatases active on short chain alkylsulphates. Microbios. 1972 Mar-Apr;5(18):87–100. [PubMed] [Google Scholar]
  5. Fitzgerald J. W., Payne W. J. The regulation of arylsulphatase formation in Pseudomonas C 12 B. Microbios. 1972 Sep-Oct;6(22):147–156. [PubMed] [Google Scholar]
  6. Fitzgerald J. W. Secondary alkylsulphatases in a strain of Comamonas terrigena. Biochem J. 1975 Aug;149(2):477–480. doi: 10.1042/bj1490477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Payne W. J., Fitzgerald J. W., Dodgson K. S. Methods for visualization of enzymes in polyacrylamide gels. Appl Microbiol. 1974 Jan;27(1):154–158. doi: 10.1128/am.27.1.154-158.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SPENCER B. Studies on sulphatases. 20. Enzymic cleavage of aryl hydrogen sulphates in the presence of H218O. Biochem J. 1958 May;69(1):155–159. doi: 10.1042/bj0690155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Spencer B. Studies on sulphatases. 25. The determination of BaSO(3)O by infrared spectroscopy. Biochem J. 1959 Nov;73(3):442–447. doi: 10.1042/bj0730442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tudball N., Thomas P. The enzymic degradation of L-serine O-sulphate by a specific system from pig liver. Studies on the mechanism of the reaction. Biochem J. 1972 Jan;126(1):187–191. doi: 10.1042/bj1260187. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES