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. 1978 Mar 1;169(3):607–615. doi: 10.1042/bj1690607

The preparation and purification of individual human pepsins by using diethylaminoethyl-cellulose.

N B Roberts, W H Taylor
PMCID: PMC1183833  PMID: 348197

Abstract

A procedure was devised for isolating human pepsins 1, 2, 3 and 5 from gastric juice by repetitive column chromatography on DEAE-cellulose. The combined yields in four different experiments varied from 14% to 90% of the total peptic activity of the starting material. The isolated individual pepsins were shown to behave as single homogeneous proteins on agar-gel electrophoresis at pH 5.0 and on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. There is preliminary evidence, requiring further study, of two other pepsins, one migrating between pepsins 1 and 2 and the other a pepsin-3 component associating closely with pepsin 5 on chromatography.

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