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. 1978 Jun 1;171(3):675–682. doi: 10.1042/bj1710675

A comparative study of the proteoglycan of growth cartilage of normal and rachitic chicks.

I R Dickson, P J Roughley
PMCID: PMC1184013  PMID: 666731

Abstract

1. Proteoglycan was isolated from growth cartilage of normal and rachitic chicks. 2. The proteoglycan from normal cartilage showed differences in chemical composition and physical properties from a comparable fraction isolated from bovine nasal cartilage. 3. The proteoglycan from rachitic-chick cartilage was of smaller size than tis normal counterpart, though of similar average chemical composition. 4. Differences between proteoglycan from normal and rachitic cartilages can be explained in terms of limited proteolytic cleavage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  3. Bisaz S., Schenk R., Kunin A. S., Russell R. G., Mühlbauer R., Fleisch H. The comparative effects of vitamin d deficiency and ethane-1-hydroxy-1,1-diphosphonate administration on the histology and glycolysis of chick epiphyseal and articular cartilage. Calcif Tissue Res. 1975 Dec 18;19(2):139–152. doi: 10.1007/BF02563998. [DOI] [PubMed] [Google Scholar]
  4. DODGSON K. S. Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters. Biochem J. 1961 Feb;78:312–319. doi: 10.1042/bj0780312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hardingham T. E., Ewins R. J., Muir H. Cartilage proteoglycans. Structure and heterogeneity of the protein core and the effects of specific protein modifications on the binding to hyaluronate. Biochem J. 1976 Jul 1;157(1):127–143. doi: 10.1042/bj1570127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hardingham T. E., Muir H. Hyaluronic acid in cartilage and proteoglycan aggregation. Biochem J. 1974 Jun;139(3):565–581. doi: 10.1042/bj1390565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hascall V. C., Oegema T. R., Brown M., Caplan A. I. Isolation and characterization of proteoglycans from chick limb bud chondrocytes grown in vitro. J Biol Chem. 1976 Jun 10;251(11):3511–3519. [PubMed] [Google Scholar]
  8. Hascall V. C., Sajdera S. W. Physical properties and polydispersity of proteoglycan from bovine nasal cartilage. J Biol Chem. 1970 Oct 10;245(19):4920–4930. [PubMed] [Google Scholar]
  9. Heinegård D., Axelsson I. Distribution of keratan sulfate in cartilage proteoglycans. J Biol Chem. 1977 Mar 25;252(6):1971–1979. [PubMed] [Google Scholar]
  10. Heinegård D. Extraction, fractionation and characterization of proteoglycans from bovine tracheal cartilage. Biochim Biophys Acta. 1972 Nov 28;285(1):181–192. doi: 10.1016/0005-2795(72)90190-0. [DOI] [PubMed] [Google Scholar]
  11. Heinegård D., Hascall V. C. Characterization of chondroitin sulfate isolated from trypsin-chymotrypsin digests of cartilage proteoglycans. Arch Biochem Biophys. 1974 Nov;165(1):427–441. doi: 10.1016/0003-9861(74)90182-9. [DOI] [PubMed] [Google Scholar]
  12. Lohmander S., Hjerpe A. Proteoglycans of mineralizing rib and epiphyseal cartilage. Biochim Biophys Acta. 1975 Sep 8;404(1):93–109. doi: 10.1016/0304-4165(75)90151-8. [DOI] [PubMed] [Google Scholar]
  13. Mayes R. W., Mason R. M., Griffin D. C. The composition of cartilage proteoglycans. An investigation using high- and low-inonic-strength extraction procedures. Biochem J. 1973 Mar;131(3):541–553. doi: 10.1042/bj1310541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McDevitt C. A., Muir H. Gel electrophoresis of proteoglycans and glycosaminoglycans on large-pore composite polyacrylamide-agarose gels. Anal Biochem. 1971 Dec;44(2):612–622. doi: 10.1016/0003-2697(71)90250-8. [DOI] [PubMed] [Google Scholar]
  15. Neville D. M., Jr Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J Biol Chem. 1971 Oct 25;246(20):6328–6334. [PubMed] [Google Scholar]
  16. Rosenberg L., Wolfenstein-Todel C., Margolis R., Pal S., Strider W. Proteoglycans from bovine proximal humeral articular cartilage. Structural basis for the polydispersity of proteoglycan subunit. J Biol Chem. 1976 Oct 25;251(20):6439–6444. [PubMed] [Google Scholar]
  17. Roughley P. J., Barrett A. J. The degradation of cartilage proteoglycans by tissue proteinases. Proteoglycan structure and its susceptibility to proteolysis. Biochem J. 1977 Dec 1;167(3):629–637. doi: 10.1042/bj1670629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Roughley P. J., Mason R. M. The electrophoretic heterogeneity of bovine nasal cartilage proteoglycans. Biochem J. 1976 Aug 1;157(2):357–367. doi: 10.1042/bj1570357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roughley P. J. The degradation of cartilage proteoglycans by tissue proteinases. Proteoglycan heterogeneity and the pathway of proteolytic degradation. Biochem J. 1977 Dec 1;167(3):639–646. doi: 10.1042/bj1670639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sajdera S. W., Hascall V. C. Proteinpolysaccharide complex from bovine nasal cartilage. A comparison of low and high shear extraction procedures. J Biol Chem. 1969 Jan 10;244(1):77–87. [PubMed] [Google Scholar]
  21. Sapolsky A. I., Keiser H., Howell D. S., Woessner J. F., Jr Metalloproteases of human articular cartilage that digest cartilage proteoglycan at neutral and acid pH. J Clin Invest. 1976 Oct;58(4):1030–1041. doi: 10.1172/JCI108526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stanescu V., Maroteaux P. Gel electrophoretic studies on proteoglycans and collagen of abnormal human growth cartilage: proteoglycan abnormalities in pseudoachondroplasia and in Kniest's disease. Pediatr Res. 1975 Oct;9(10):779–782. doi: 10.1203/00006450-197510000-00006. [DOI] [PubMed] [Google Scholar]
  23. Stanescu V., Maroteaux P., Sobczak E. Proteoglycan populations of baboon (Papio papio) articular cartilage. Biochem J. 1977 Apr 1;163(1):103–109. doi: 10.1042/bj1630103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Toole B. P., Kang A. H., Trelstad R. L., Gross J. Collagen heterogeneity within different growth regions of long bones of rachitic and non-rachitic chicks. Biochem J. 1972 May;127(4):715–720. doi: 10.1042/bj1270715. [DOI] [PMC free article] [PubMed] [Google Scholar]

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