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. 1969 Jun;113(1):29–37. doi: 10.1042/bj1130029

Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance

Janet M Sterlini 1, J Mandelstam 1
PMCID: PMC1184601  PMID: 4185146

Abstract

1. Experiments to determine the point of commitment to sporulation were carried out by restoring nutrients at different times to suspensions of sporulating Bacillus subtilis. 2. No single point of commitment to the process as a whole was found. Instead, the cells became committed in turn to the following successive events connected with sporulation: formation of alkaline phosphatase, development of refractility, synthesis of dipicolinic acid and development of heat-resistance. 3. Each point of commitment was followed within about 30min. by a period in which the event concerned ceased to be inhibited by actinomycin D. 4. The implication of these results is that each point of commitment is probably due to the formation of a species of long-lived messenger RNA and that, in any case, sporulation is regulated at the level of both transcription and translation. 5. It is also shown that sporulation and growth are perhaps not mutually exclusive functions and that histidase, an enzyme typical of the vegetative state, can be induced in sporulating suspensions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARONSON A. I., ROSASDELVALLE M. RNA AND PROTEIN SYNTHESIS REQUIRED FOR BACTERIAL SPORE FORMATION. Biochim Biophys Acta. 1964 Jun 22;87:267–276. doi: 10.1016/0926-6550(64)90222-1. [DOI] [PubMed] [Google Scholar]
  2. BALASSA G. RENOUVELLEMENT DE L'ACIDE RIBONUCL'EIQUE AU COURS DE LA SPORULATION DE BACILLUS SUBTILIS. Biochim Biophys Acta. 1963 Nov 22;76:410–416. doi: 10.1016/0006-3002(63)90060-x. [DOI] [PubMed] [Google Scholar]
  3. DONNELLAN J. E., Jr, NAGS E. H., LEVINSON H. S. CHEMICALLY DEFINED, SYNTHETIC MEDIA FOR SPORULATION AND FOR GERMINATION AND GROWTH OF BACILLUS SUBTILIS. J Bacteriol. 1964 Feb;87:332–336. doi: 10.1128/jb.87.2.332-336.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FOSTER J. W. Morphogenesis in bacteria: some aspects of spore formation. Q Rev Biol. 1956 Jun;31(2):102–118. doi: 10.1086/401259. [DOI] [PubMed] [Google Scholar]
  5. HARDWICK W. A., FOSTER J. W. On the nature of sporogenesis in some aerobic bacteria. J Gen Physiol. 1952 Jul;35(6):907–927. doi: 10.1085/jgp.35.6.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HARTWELL L. H., MAGASANIK B. THE MOLECULAR BASIS OF HISTIDASE INDUCTION IN BACILLUS SUBTILIS. J Mol Biol. 1963 Oct;7:401–420. doi: 10.1016/s0022-2836(63)80033-9. [DOI] [PubMed] [Google Scholar]
  7. JANSSEN F. W., LUND A. J., ANDERSON L. E. Colorimetric assay for dipicolinic acid in bacterial spores. Science. 1958 Jan 3;127(3288):26–27. doi: 10.1126/science.127.3288.26. [DOI] [PubMed] [Google Scholar]
  8. Kay D., Warren S. C. Sporulation in Bacillus subtilis. Morphological changes. Biochem J. 1968 Oct;109(5):819–824. doi: 10.1042/bj1090819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LEVINTHAL C., KEYNAN A., HIGA A. Messenger RNA turnover and protein synthesis in B. subtilis inhibited by actinomycin D. Proc Natl Acad Sci U S A. 1962 Sep 15;48:1631–1638. doi: 10.1073/pnas.48.9.1631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mandelstam J., Waites W. M. Sporulation in Bacillus subtilis. The role of exoprotease. Biochem J. 1968 Oct;109(5):793–801. doi: 10.1042/bj1090793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SZULMAJSTER J. BIOCHIMIE DE LA SPOROG'EN'ESE CHEZ B. SUBTILIS. Bull Soc Chim Biol (Paris) 1964;46:443–481. [PubMed] [Google Scholar]
  12. SZULMAJSTER J., CANFIELD R. E., BLICHARSKA J. [Action of actinomycin D on the sporulation of Bacillus subtilis]. C R Hebd Seances Acad Sci. 1963 Feb 25;256:2057–2060. [PubMed] [Google Scholar]
  13. TORRIANI A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta. 1960 Mar 11;38:460–469. doi: 10.1016/0006-3002(60)91281-6. [DOI] [PubMed] [Google Scholar]
  14. Warren S. C. Sporulation in Bacillus subtilis. Biochemical changes. Biochem J. 1968 Oct;109(5):811–818. doi: 10.1042/bj1090811. [DOI] [PMC free article] [PubMed] [Google Scholar]

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