Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1969 Jun;113(1):167–174. doi: 10.1042/bj1130167

The integration of donor and recipient deoxyribonucleic acid during transformation of Bacillus subtilis

S R Ayad 1, G R Barker 1
PMCID: PMC1184616  PMID: 4979894

Abstract

1. DNA labelled with 5-bromo[3H]uracil was used to transform auxotrophic strains of Bacillus subtilis. 2. After various times of incubation, DNA was extracted from the transformed culture and subjected to equilibrium sedimentation in caesium chloride gradients. 3. In addition to heavy donor DNA and light recipient DNA, a component with an intermediate density was found and is believed to consist of a biological hybrid of donor and recipient. 4. The component of intermediate density was isolated and found to possess activity in transformation derived from both donor and recipient strains. 5. Denaturation of the component of intermediate density followed by centrifugation gave only one component, indicating that integration had occurred in both strands of the recipient DNA. 6. No integrated band was observed after uptake by competent cells of B. subtilis of heavy DNA prepared from Escherichia coli.

Full text

PDF
172

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akrigg A., Ayad S. R., Barker G. R. The nature of a competence-inducing factor in Bacillus subtilis. Biochem Biophys Res Commun. 1967 Sep 27;28(6):1062–1067. doi: 10.1016/0006-291x(67)90090-3. [DOI] [PubMed] [Google Scholar]
  2. BODMER W. F., GANESAN A. T. BIOCHEMICAL AND GENETIC STUDIES OF INTEGRATION AND RECOMBINATION IN BACILLUS SUBTILIS TRANSFORMATION. Genetics. 1964 Oct;50:717–738. doi: 10.1093/genetics/50.4.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Becker E. F., Jr Structure and function of cross-linked DNA. II. Loss of reversibility of HNO2-cross-linked DNA during transformation of Bacillus subtilis. Biochim Biophys Acta. 1967 Jun 20;142(1):238–244. [PubMed] [Google Scholar]
  4. FOX M. S., ALLEN M. K. ON THE MECHANISM OF DEOXYRIBONUCLEATE INTEGRATION IN PNEUMOCOCCAL TRANSFORMATION. Proc Natl Acad Sci U S A. 1964 Aug;52:412–419. doi: 10.1073/pnas.52.2.412. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harris W. J., Barr G. C. Some properties of deoxyribonucleic acid in competent Bacillus subtilis. Biochem J. 1968 Dec;110(3):38P–38P. doi: 10.1042/bj1100038pb. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hewitt R., Suit J. C., Billen D. Utilization of 5-bromouracil by thymineless bacteria. J Bacteriol. 1967 Jan;93(1):86–89. doi: 10.1128/jb.93.1.86-89.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kammen H. O., Beloff R. H., Canellakis E. S. Transformation in Bacillus subtilis. I. Role of amino acids in stabilization of transformants. Biochim Biophys Acta. 1966 Jul 20;123(1):39–55. [PubMed] [Google Scholar]
  8. LACKS S. Molecular fate of DNA in genetic transformation of Pneumococcus. J Mol Biol. 1962 Jul;5:119–131. doi: 10.1016/s0022-2836(62)80067-9. [DOI] [PubMed] [Google Scholar]
  9. NESTER E. W., STOCKER B. A. BIOSYNTHETIC LATENCY IN EARLY STAGES OF DEOXYRIBONUCLEIC ACIDTRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1963 Oct;86:785–796. doi: 10.1128/jb.86.4.785-796.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Notani N., Goodgal S. H. On the nature of recombinants formed during transformation in Hemophilus influenzae. J Gen Physiol. 1966 Jul;49(6):197–209. doi: 10.1085/jgp.49.6.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PENE J. J., ROMIG W. R. ON THE MECHANISM OF GENETIC RECOMBINATION IN TRANSFORMING BACILLUS SUBTILIS. J Mol Biol. 1964 Jul;9:236–245. doi: 10.1016/s0022-2836(64)80103-0. [DOI] [PubMed] [Google Scholar]
  12. SUEOKA N., CHENG T. Y. Fractionation of nucleic acids with the methylated albumin column. J Mol Biol. 1962 Mar;4:161–172. doi: 10.1016/s0022-2836(62)80048-5. [DOI] [PubMed] [Google Scholar]
  13. Singh R. N., Pitale M. P. Competence and deoxyribonucleic acid uptake in Bacillus subtilis. J Bacteriol. 1968 Mar;95(3):864–866. doi: 10.1128/jb.95.3.864-866.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stuy J. H. Fate of transforming DNA in the Haemophilus influenzae transformation system. J Mol Biol. 1965 Sep;13(2):554–570. doi: 10.1016/s0022-2836(65)80117-6. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES