Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1969 Jul;113(4):707–712. doi: 10.1042/bj1130707

Selective effect of hormones on nucleic acid metabolism during germination of pear embryos

A A Khan 1, C E Heit 1
PMCID: PMC1184754  PMID: 5386193

Abstract

1. The effect of hormones on 32P incorporation into various RNA fractions in germinating pear embryos was studied by fractionation on methylated albumin–kieselguhr columns. Abscisic acid inhibited labelling of soluble RNA, DNA–RNA hybrid and light-ribosomal RNA fractions with 32P and this effect was reversed by both kinetin and gibberellic acid. 2. Kinetin reversed the inhibition by abscisic acid of 32P incorporation into total ribosomal RNA and appeared to promote labelling of heavy-ribosomal RNA. Gibberellic acid was more active than kinetin in reversing the inhibition by abscisic acid of labelling of the DNA–RNA hybrid fraction with 32P, but in contrast with kinetin appeared to increase further the inhibition by abscisic acid of labelling of total ribosomal RNA. 3. The percentage of radioactivity in various RNA fractions showed marked variation in response to hormones. 4. The pattern of labelling of RNA in pear embryos during reversal of inhibition by abscisic acid with a combination of kinetin and gibberellic acid was similar to that after cold-treatment of dormant pear embryos. This is suggestive of hormonal interplay in dormancy release by cold-treatment in pear embryos.

Full text

PDF
708

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHERRY J. H. ASSOCIATION OF RAPIDLY METABOLIZED DNA AND RNA. Science. 1964 Nov 20;146(3647):1066–1069. doi: 10.1126/science.146.3647.1066. [DOI] [PubMed] [Google Scholar]
  2. Carpenter W. J., Cherry J. H. Effects of benzyladenine on accumulation of 32P into nucleic acids of peanut cotyledons. Biochim Biophys Acta. 1966 Mar 21;114(3):640–642. doi: 10.1016/0005-2787(66)90115-8. [DOI] [PubMed] [Google Scholar]
  3. HAYASHI M., SPIEGELMAN S. The selective synthesis of informational RNA in bacteria. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1564–1580. doi: 10.1073/pnas.47.10.1564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. INGLE J., KEY J. L., HOLM R. E. DEMONSTRATION AND CHARACTERIZATION OF A DNA-LIKE RNA IN EXCISED PLANT TISSUE. J Mol Biol. 1965 Apr;11:730–746. doi: 10.1016/s0022-2836(65)80031-6. [DOI] [PubMed] [Google Scholar]
  5. Ingle J., Key J. L. A re-evaluation of the fractionation of high molecular weight RNA by MAK chromatography. Biochem Biophys Res Commun. 1968 Mar 27;30(6):711–716. doi: 10.1016/0006-291x(68)90571-8. [DOI] [PubMed] [Google Scholar]
  6. Jarvis B. C., Frankland B., Cherry J. H. Increased DNA template and RNA polymerase associated with the breaking of seed dormancy. Plant Physiol. 1968 Oct;43(10):1734–1736. doi: 10.1104/pp.43.10.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Key J. L., Ingle J. REQUIREMENT FOR THE SYNTHESIS OF DNA-LIKE RNA FOR GROWTH OF EXCISED PLANT TISSUE. Proc Natl Acad Sci U S A. 1964 Dec;52(6):1382–1388. doi: 10.1073/pnas.52.6.1382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Khan A. A., Heit C. E., Lippold P. C. Increase in nucleic acid synthesizing capacity during cold treatment of dormant pear embryos. Biochem Biophys Res Commun. 1968 Nov 8;33(3):391–396. doi: 10.1016/0006-291x(68)90583-4. [DOI] [PubMed] [Google Scholar]
  9. Khan A. A. Inhibition of Gibberellic Acid-induced Germination by Abscisic Acid and Reversal by Cytokinins. Plant Physiol. 1968 Sep;43(9):1463–1465. doi: 10.1104/pp.43.9.1463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  11. Osborne D. J. Effect of Kinetin on Protein & Nucleic Acid Metabolism in Xanthium Leaves During Senescence. Plant Physiol. 1962 Sep;37(5):595–602. doi: 10.1104/pp.37.5.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sobota A. E., Leaver C. J., Key J. L. A detailed evaluation of the possible contribution of bacteria to radioactive precursor incorporation into nucleic acids of plant tissues. Plant Physiol. 1968 Jun;43(6):907–913. doi: 10.1104/pp.43.6.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sondheimer E., Galson E. C. Effects of Abscisin II and Other Plant Growth Substances on Germination of Seeds with Stratification Requirements. Plant Physiol. 1966 Oct;41(8):1397–1398. doi: 10.1104/pp.41.8.1397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sondheimer E., Tzou D. S., Galson E. C. Abscisic Acid levels and seed dormancy. Plant Physiol. 1968 Sep;43(9):1443–1447. doi: 10.1104/pp.43.9.1443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. YANKOFSKY S. A., SPIEGELMAN S. The identification of the ribosomal RNA cistron by sequence complementarity. I. Specificity of complex formation. Proc Natl Acad Sci U S A. 1962 Jun 15;48:1069–1078. doi: 10.1073/pnas.48.6.1069. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES