Abstract
1. To deplete them of ATP the tumour cells were starved at 37° in a Ringer solution containing 33m-equiv. of Na+/l., 131m-equiv. of Li+/l., 2mM-sodium cyanide and 0·1mm-ouabain. The cellular content of K+ was largely replaced by Li+, but cellular [Na+] remained near 33m-equiv./l. 2. The addition of 12mm-glycine to the system caused cellular [Na+] to increase, during the next 4min., by about 4m-equiv./l., so that it slightly exceeded extracellular [Na+]. This occurred in parallel with the absorption of glycine. 3. The cellular K+ content fell by an amount representing about 10% of the amount of Na+ absorbed. 4. The results provide a clear demonstration that the flow of glycine into the cells is linked to a parallel movement of Na+; K+ appears to play a facultative role in the carrier system, whereas Li+ is almost inert. 5. The effects produced by glycine were not reproduced by l-arabinose.
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Selected References
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