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. 1969 Dec;115(4):849–856. doi: 10.1042/bj1150849

Some properties of cytochrome b5 from liver microsomes of man, monkey, pig and chicken

F G Nóbrega 1, P S Araujo 1, M Pasetto 1, I Raw 1
PMCID: PMC1185215  PMID: 4982419

Abstract

1. Cytochrome b5 was released from liver microsomes of man, monkey, pig and chicken by incubation with a crude lipase preparation. 2. By using DEAE-cellulose chromatography, ammonium sulphate fractionation, Sephadex-gel filtration and a final gradient elution on DEAE-Sephadex A-50, cytochromes b5 were obtained from the four species studied, all possessing similar spectral properties. 3. Stokes radii of the cytochromes were measured by gel filtration. 4. N-Terminal amino acids for the different cytochromes were serine for man and monkey, alanine for pig and glycine for chicken. 5. Amino acid analyses of the cytochromes are presented. 6. Peptide `fingerprint' patterns of tryptic digests of the different cytochromes are discussed and clearly show increasing similarity for more closely related species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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