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. 1969 Dec;115(5):881–887. doi: 10.1042/bj1150881

The synthesis of nicotinamide–adenine dinucleotide and poly(adenosine diphosphate ribose) in various classes of rat liver nuclei

M E Haines 1, I R Johnston 1, A P Mathias 1, D Ridge 1
PMCID: PMC1185229  PMID: 4311824

Abstract

1. The activities of NMN adenylyltransferase and an enzyme that synthesizes poly (ADP-ribose) from NAD were investigated in the various classes of rat liver nuclei fractionated by zonal centrifugation. 2. The highest specific activities of these two nuclear enzymes occur in different classes of nuclei. In very young and in mature rats it was shown that a correlation exists between DNA synthesis and NMN adenylyltransferase activity, but in rats of intermediate age this correlation is less evident. The highest activities of the enzyme that catalyses formation of poly (ADP-ribose) are in the nuclei involved in the synthesis of RNA. 3. The significance of these results in relation to NAD metabolism is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALTUS E. Rôle du noyau cellulaire dans le métabolisme des coenzymes. Arch Int Physiol Biochim. 1956 Jan;64(1):124–125. doi: 10.3109/13813455609150215. [DOI] [PubMed] [Google Scholar]
  2. BRANSTER M. V., MORTON R. K. Comparative rates of synthesis of diphosphopyridine nucleotide by normal and tumour tissue from mouse mammary gland; studies with isolated nuclei. Biochem J. 1956 Aug;63(4):640–646. doi: 10.1042/bj0630640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CAIGER P., MORTON R. K., FILSELL O. H., JARRETT I. G. A comparative study of nicotinamide nucleotide coenzymes during growth of the sheep and rat. Biochem J. 1962 Nov;85:351–359. doi: 10.1042/bj0850351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CHAYKIN S., DAGANI M., JOHNSON L., SAMLI M., BATTAILE J. THE FATE OF NICOTINAMIDE IN THE MOUSE; TISSUE METABOLITES. Biochim Biophys Acta. 1965 May 4;100:351–365. doi: 10.1016/0304-4165(65)90004-8. [DOI] [PubMed] [Google Scholar]
  5. Carriere R. The growth of liver parenchymal nuclei and its endocrine regulation. Int Rev Cytol. 1969;25:201–277. doi: 10.1016/s0074-7696(08)60204-1. [DOI] [PubMed] [Google Scholar]
  6. Greenbaum A. L., Pinder S. Changes in the activities of enzymes of the biosynthetic pathway of the nicotinamide nucleotides in rat mammary gland during the lactation cycle. Biochem J. 1968 Mar;107(1):63–67. doi: 10.1042/bj1070063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HOGEBOOM G. H., SCHNEIDER W. C. Cytochemical studies. VI. The synthesis of diphosphopyridine nucleotide by liver cell nuclei. J Biol Chem. 1952 May;197(2):611–620. [PubMed] [Google Scholar]
  8. Honjo T., Nishizuka Y., Hayaishi O. Diphtheria toxin-dependent adenosine diphosphate ribosylation of aminoacyl transferase II and inhibition of protein synthesis. J Biol Chem. 1968 Jun 25;243(12):3553–3555. [PubMed] [Google Scholar]
  9. Johnston I. R., Mathias A. P., Pennington F., Ridge D. Distribution of RNA polymerase activity among the various classes of liver nuclei. Nature. 1968 Nov 16;220(5168):668–672. doi: 10.1038/220668a0. [DOI] [PubMed] [Google Scholar]
  10. Johnston I. R., Mathias A. P., Pennington F., Ridge D. The fractionation of nuclei from mammalian cells by zonal centrifugation. Biochem J. 1968 Aug;109(1):127–135. doi: 10.1042/bj1090127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kaufmann E. A., Traub A., Teitz Y. Isolation of different-sized nucleoli from normal and regenerating rat liver. Exp Cell Res. 1968 Jan;49(1):215–218. doi: 10.1016/0014-4827(68)90537-5. [DOI] [PubMed] [Google Scholar]
  12. Lindahl T., Edelman G. M. Polynucleotide ligase from myeloid and lymphoid tissues. Proc Natl Acad Sci U S A. 1968 Oct;61(2):680–687. doi: 10.1073/pnas.61.2.680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MORTON R. K. Enzymic synthesis of coenzyme I in relation to chemical control of cell growth. Nature. 1958 Feb 22;181(4608):540–542. doi: 10.1038/181540a0. [DOI] [PubMed] [Google Scholar]
  14. MYERS D. K. Effects of x-irradiation on enzyme synthesis during liver regeneration. Can J Biochem Physiol. 1962 May;40:619–630. [PubMed] [Google Scholar]
  15. MacGregor R. R., Mahler H. R. RNA synthesis in intact rat liver nuclei. Arch Biochem Biophys. 1967 Apr;120(1):136–157. doi: 10.1016/0003-9861(67)90607-8. [DOI] [PubMed] [Google Scholar]
  16. Nakazawa K., Ueda K., Honjo T., Yoshihara K., Nishizuka Y., Hayaishi O. Nicotinamide adenine dinucleotide glycohydrolases and poly adenosine diphosphate ribose synthesis in rat liver. Biochem Biophys Res Commun. 1968 Jul 26;32(2):143–149. doi: 10.1016/0006-291x(68)90360-4. [DOI] [PubMed] [Google Scholar]
  17. Nishizuka Y., Ueda K., Honjo T., Hayaishi O. Enzymic adenosine diphosphate ribosylation of histone and poly adenosine diphosphate ribose synthesis in rat liver nuclei. J Biol Chem. 1968 Jul 10;243(13):3765–3767. [PubMed] [Google Scholar]
  18. Nishizuka Y., Ueda K., Nakazawa K., Hayaishi O. Studies on the polymer of adenosine diphosphate ribose. I. Enzymic formation from nicotinamide adenine dinuclotide in mammalian nuclei. J Biol Chem. 1967 Jul 10;242(13):3164–3171. [PubMed] [Google Scholar]
  19. Nishizuka Y., Ueda K., Nakazawa L., Reeder R. H., Honjo T. Poly adenosine diphosphate ribose synthesis and nicotinamide adenine dinucleotide transglycosidases. J Vitaminol (Kyoto) 1968 Mar 5;14(Suppl):143–152. doi: 10.5925/jnsv1954.14.supplement_143. [DOI] [PubMed] [Google Scholar]
  20. Oishi M. Studies of DNA replication in vivo, II. Evidence for the second intermediate. Proc Natl Acad Sci U S A. 1968 Jun;60(2):691–698. doi: 10.1073/pnas.60.2.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Okazaki R., Okazaki T., Sakabe K., Sugimoto K., Sugino A. Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains. Proc Natl Acad Sci U S A. 1968 Feb;59(2):598–605. doi: 10.1073/pnas.59.2.598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Olivera B. M., Hall Z. W., Lehman I. R. Enzymatic joining of polynucleotides, V. A DNA-adenylate intermediate in the polynucleotide-joining reaction. Proc Natl Acad Sci U S A. 1968 Sep;61(1):237–244. doi: 10.1073/pnas.61.1.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Otake H., Miwa M., Fujimura S., Sugimura T. Binding of ADP-ribose polymer with histone. J Biochem. 1969 Jan;65(1):145–146. [PubMed] [Google Scholar]
  24. Reeder R. H., Ueda K., Honjo T., Nishizuka Y., Hayaishi O. Studies on the polymer of adenosine diphosphate ribose. II. Characterization of the polymer. J Biol Chem. 1967 Jul 10;242(13):3172–3179. [PubMed] [Google Scholar]
  25. Sakabe K., Okazaki R. A unique property of the replicating region of chromosomal DNA. Biochim Biophys Acta. 1966 Dec 21;129(3):651–654. doi: 10.1016/0005-2787(66)90088-8. [DOI] [PubMed] [Google Scholar]
  26. Siebert G., Villalobos J., Jr, Ro T. S., Steele W. J., Lindenmayer G., Adams H., Busch H. Enzymatic studies on isolated nucleoli of rat liver. J Biol Chem. 1966 Jan 10;241(1):71–78. [PubMed] [Google Scholar]
  27. Sugimoto K., Okazaki T., Okazaki R. Mechanism of DNA chain growth, II. Accumulation of newly synthesized short chains in E. coli infected with ligase-defective T4 phages. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1356–1362. doi: 10.1073/pnas.60.4.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sugimura T., Fujimura S., Hasegawa S., Kawamura Y. Polymerization of the adenosine 5'-diphosphate ribose moiety of NAD by rat liver nuclear enzyme. Biochim Biophys Acta. 1967 Apr 18;138(2):438–441. doi: 10.1016/0005-2787(67)90507-2. [DOI] [PubMed] [Google Scholar]
  29. Widnell C. C., Tata J. R. A procedure for the isolation of enzymically active rat-liver nuclei. Biochem J. 1964 Aug;92(2):313–317. doi: 10.1042/bj0920313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. ZATMAN L. J., KAPLAN N. O., COLOWICK S. P. Inhibition of spleen diphosphopyridine nucleotidase by nicotinamide, an exchange reaction. J Biol Chem. 1953 Jan;200(1):197–212. [PubMed] [Google Scholar]
  31. Zimmerman S. B., Little J. W., Oshinsky C. K., Gellert M. Enzymatic joining of DNA strands: a novel reaction of diphosphopyridine nucleotide. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1841–1848. doi: 10.1073/pnas.57.6.1841. [DOI] [PMC free article] [PubMed] [Google Scholar]

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