Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1970 Jan;116(1):83–92. doi: 10.1042/bj1160083

The preparation of iodinated vancomycin and its distribution in bacteria treated with the antibiotic

H R Perkins 1, M Nieto 1
PMCID: PMC1185327  PMID: 5411430

Abstract

Vancomycin was radioactively labelled by iodination with 125I.Iodinated vancomycin was only a little less potent as an antibiotic than vancomycin itself. It was shown, both by chromatography and differential absorption measurements, to combine with acyl-d-alanyl-d-alanine residues. Radioactive vancomycin was used to follow the fate of the antibiotic in bacteria that had been subjected to the least concentration required to inhibit growth. Most of the radioactivity was in the cell walls, although some was found in the membrane fraction. The latter proportion increased during longer incubations with the antibiotic. Pre-formed protoplasts adsorbed very little vancomycin. Mg2+ removed labelled vancomycin from the mucopeptide of Bacillus licheniformis, but had little effect on the antibiotic adsorbed on Micrococcus lysodeikticus, either in vivo or on previously isolated cell walls. Specific peptide was shown to compete with cell walls for vancomycin and it also extracted from cell-wall samples the labelled compound that had been adsorbed on M. lysodeikticus living cells.

Full text

PDF
84

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON J. S., MATSUHASHI M., HASKIN M. A., STROMINGER J. L. LIPID-PHOSPHOACETYLMURAMYL-PENTAPEPTIDE AND LIPID-PHOSPHODISACCHARIDE-PENTAPEPTIDE: PRESUMED MEMBRANE TRANSPORT INTERMEDIATES IN CELL WALL SYNTHESIS. Proc Natl Acad Sci U S A. 1965 Apr;53:881–889. doi: 10.1073/pnas.53.4.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson J. S., Matsuhashi M., Haskin M. A., Strominger J. L. Biosythesis of the peptidoglycan of bacterial cell walls. II. Phospholipid carriers in the reaction sequence. J Biol Chem. 1967 Jul 10;242(13):3180–3190. [PubMed] [Google Scholar]
  3. BEST G. K., DURHAM N. N. EFFECT OF VANCOMYCIN ON BACILLUS SUBTILIS. Arch Biochem Biophys. 1964 Apr;105:120–125. doi: 10.1016/0003-9861(64)90242-5. [DOI] [PubMed] [Google Scholar]
  4. Best G. K., Durham N. N. Vancomycin adsorption to Bacillus subtilis cell walls. Arch Biochem Biophys. 1965 Sep;111(3):685–691. doi: 10.1016/0003-9861(65)90250-x. [DOI] [PubMed] [Google Scholar]
  5. Chatterjee A. N., Perkins H. R. Compounds formed between nucleotides related to the biosynthesis of bacterial cell wall and vancomycin. Biochem Biophys Res Commun. 1966 Aug 12;24(3):489–494. doi: 10.1016/0006-291x(66)90188-4. [DOI] [PubMed] [Google Scholar]
  6. Higashi Y., Strominger J. L., Sweeley C. C. Structure of a lipid intermediate in cell wall peptidoglycan synthesis: a derivative of a C55 isoprenoid alcohol. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1878–1884. doi: 10.1073/pnas.57.6.1878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hughes R. C. The cell wall of Bacillus licheniformis N.C.T.C. 6346. Isolation of low-molecular-weight fragments from the soluble mucopeptide. Biochem J. 1968 Jan;106(1):49–59. doi: 10.1042/bj1060049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. JORDAN D. C. Effect of vancomycin on the synthesis of the cell wall mucopeptide of Staphylococcus aureus. Biochem Biophys Res Commun. 1961 Nov 20;6:167–170. doi: 10.1016/0006-291x(61)90122-x. [DOI] [PubMed] [Google Scholar]
  9. MARSHALL F. J. STRUCTURE STUDIES ON VANCOMYCIN. J Med Chem. 1965 Jan;8:18–22. doi: 10.1021/jm00325a004. [DOI] [PubMed] [Google Scholar]
  10. McFARLANE A. S. Efficient trace-labelling of proteins with iodine. Nature. 1958 Jul 5;182(4627):53–53. doi: 10.1038/182053a0. [DOI] [PubMed] [Google Scholar]
  11. McFarlane A. S. IN VIVO BEHAVIOR OF I-FIBRINOGEN. J Clin Invest. 1963 Mar;42(3):346–361. doi: 10.1172/JCI104721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
  13. Perkins H. R. Specificity of combination between mucopeptide precursors and vancomycin or ristocetin. Biochem J. 1969 Jan;111(2):195–205. doi: 10.1042/bj1110195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. REYNOLDS P. E. Studies on the mode of action of vancomycin. Biochim Biophys Acta. 1961 Sep 16;52:403–405. doi: 10.1016/0006-3002(61)90698-9. [DOI] [PubMed] [Google Scholar]
  15. Sinha R. K., Neuhaus R. C. Reversal of the vancomycin inhibition of peptidoglycan synthesis by cell walls. J Bacteriol. 1968 Aug;96(2):374–382. doi: 10.1128/jb.96.2.374-382.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES