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. 1997 Feb;72(2 Pt 1):858–865. doi: 10.1016/s0006-3495(97)78720-4

Graphical Evaluation of Alkylation of Myosin's SH1 and SH2

The N-Phenylmaleimide Reaction

Ling Xie *, Wan Xia Li *, Vincent A Barnett #, Mark Schoenberg *
PMCID: PMC1185609  PMID: 9017211

Abstract

Previous assertions about the effect of alkylation of SH1 and SH2 on the myosin high-salt calcium and EDTA ATPases have been summarized, and a simple procedure for obtaining the fractional labeling of SH1 and SH2 after treatment of myosin with alkylating agents has been derived. A simple graphical procedure for illustrating the degree of preference of a particular alkylating agent for SH1 over SH2 has also been developed. The procedures we developed were validated by applying them to two previously studied compounds, 4-(2-iodoacetamido)-TEMPO and 2,4-dinitrofluorobenzine, and then were used to determine a procedure for maximizing the extent of labeling of SH1 alone by N-phenylmaleimide, a compound not previously studied in this manner. It was found that ∼80% of the SH1 sites could be alkylated without significant alkylation of SH2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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