Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1978 Jul 1;173(1):333–336. doi: 10.1042/bj1730333

Blood-group-I activity associated with band 3, the major intrinsic membrane protein of human erythrocytes.

R A Childs, T Feizi, M Fukuda, S I Hakomori
PMCID: PMC1185778  PMID: 687374

Abstract

Affinity chromatography of radioiodinated solubilized erythrocyte stroma and of radioiodinated purified Band 3 on an anti-(blood-group I)-adsorbent column showed blood-group-I activity associated with a subpopulation of Band 3. The specificity of binding was confirmed by inhibition with known blood-group substances in radioimmunoassays.

Full text

PDF
336

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bretscher M. S. A major protein which spans the human erythrocyte membrane. J Mol Biol. 1971 Jul 28;59(2):351–357. doi: 10.1016/0022-2836(71)90055-6. [DOI] [PubMed] [Google Scholar]
  2. Butters T. D., Hughes R. C. Surface labelling for human tumour KB cells. Iodination and fractionation of membrane glycoproteins. Biochem J. 1975 Jul;150(1):59–69. doi: 10.1042/bj1500059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cabantchik Z. I., Rothstein A. Membrane proteins related to anion permeability of human red blood cells. II. Effects of proteolytic enzymes on disulfonic stilbene sites of surface proteins. J Membr Biol. 1974;15(3):227–248. doi: 10.1007/BF01870089. [DOI] [PubMed] [Google Scholar]
  4. DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
  5. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  6. Feizi T., Cederqvist L. L., Childs R. The blood group I and i antigens of amniotic fluid. I. Association of I and i antigens with blood group A, B and H antigens. Br J Haematol. 1975 Aug;30(4):489–497. doi: 10.1111/j.1365-2141.1975.tb01863.x. [DOI] [PubMed] [Google Scholar]
  7. Feizi T., Kabat E. A., Vicari G., Anderson B., Marsh W. L. Immunochemical studies on blood groups.XLIX. The I antigen complex: specificity differences among anti-I sera revealed by quantitative precipitin studies; partial structure of the I determinant specific for one anti-I serum. J Immunol. 1971 Jun;106(6):1578–1592. [PubMed] [Google Scholar]
  8. Findlay J. B. The receptor proteins for concanavalin A and Lens culinaris phytohemagglutinin in the membrane of the human erythrocyte. J Biol Chem. 1974 Jul 25;249(14):4398–4403. [PubMed] [Google Scholar]
  9. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gardas A. Studies on the I-blood-group-active sites on macro-glycolipids from human erythrocytes. Eur J Biochem. 1976 Sep;68(1):185–191. doi: 10.1111/j.1432-1033.1976.tb10777.x. [DOI] [PubMed] [Google Scholar]
  11. Jenkins R. E., Tanner J. A. The major human erythrocyte membrane protein. Evidence for an S-shaped structure which traverses the membrane twice and contains a duplicated set of sites. Biochem J. 1975 Jun;147(3):393–399. doi: 10.1042/bj1470393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kościelak J., Miller-Podraza H., Krauze R., Piasek A. Isolation and characterization of poly(glycosyl)ceramides (megaloglycolipids) with A, H and I blood-group activities. Eur J Biochem. 1976 Dec;71(1):9–18. doi: 10.1111/j.1432-1033.1976.tb11083.x. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Lecomte J., Feizi T. A common idiotype on human macroglobulins with anti-I and anti-i specificity. Clin Exp Immunol. 1975 May;20(2):287–302. [PMC free article] [PubMed] [Google Scholar]
  16. Marchesi V. T., Furthmayr H., Tomita M. The red cell membrane. Annu Rev Biochem. 1976;45:667–698. doi: 10.1146/annurev.bi.45.070176.003315. [DOI] [PubMed] [Google Scholar]
  17. Steck T. L., Ramos B., Strapazon E. Proteolytic dissection of band 3, the predominant transmembrane polypeptide of the human erythrocyte membrane. Biochemistry. 1976 Mar 9;15(5):1153–1161. doi: 10.1021/bi00650a030. [DOI] [PubMed] [Google Scholar]
  18. Watanabe K., Laine R. A., Hakomori S. I. On neutral fucoglycolipids having long, branched carbohydrate chains: H-active and I-active glycosphingolipids of human erythrocyte membranes. Biochemistry. 1975 Jun 17;14(12):2725–2733. doi: 10.1021/bi00683a026. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES