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. 1978 Sep 15;174(3):1059–1062. doi: 10.1042/bj1741059

The adenylate cyclase-activating activity of cholera toxin is not associated with a nicotinamide--adenine dinucleotide glycohydrolase activity.

R M Tait, S van Heyningen
PMCID: PMC1186012  PMID: 215119

Abstract

The NAD+ glycohydrolase activity of cholera-toxin samples can be separated from their adenylate cyclase-activating activity by polyacrylamide-gel electrophoresis and is inhibited by sodium dodecyl sulphate (which does not inhibit the action of toxin on cells), but not by antibodies to pure toxin. It is therefore probably not a true property of the toxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apps D. K., Nairn A. C. The equilibrium constant and the reversibility of the reaction catalysed by nicotinamide-adenine dinucleotide kinase from pigeon liver. Biochem J. 1977 Oct 1;167(1):87–93. doi: 10.1042/bj1670087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COLOWICK S. P., KAPLAN N. O., CIOTTI M. M. The reaction of pyridine nucleotide with cyanide and its analytical use. J Biol Chem. 1951 Aug;191(2):447–459. [PubMed] [Google Scholar]
  3. Cassel D., Selinger Z. Mechanism of adenylate cyclase activation by cholera toxin: inhibition of GTP hydrolysis at the regulatory site. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3307–3311. doi: 10.1073/pnas.74.8.3307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. Fricke U. Tritosol: a new scintillation cocktail based on Triton X-100. Anal Biochem. 1975 Feb;63(2):555–558. doi: 10.1016/0003-2697(75)90379-6. [DOI] [PubMed] [Google Scholar]
  6. Gill D. M. Multiple roles of erythrocyte supernatant in the activation of adenylate cyclase by Vibrio cholerae toxin in vitro. J Infect Dis. 1976 Mar;133 (Suppl):55–63. doi: 10.1093/infdis/133.supplement_1.s55. [DOI] [PubMed] [Google Scholar]
  7. Heyningen S Van Cholera toxin: interaction of subunits with ganglioside GM1. Science. 1974 Feb 15;183(4125):656–657. doi: 10.1126/science.183.4125.656. [DOI] [PubMed] [Google Scholar]
  8. Heyningen S. Activation by cholera toxin of adenylate cyclase solubilized from rat liver. Biochem J. 1976 Sep 1;157(3):785–787. doi: 10.1042/bj1570785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keirns J. J., Wheeler M. A., Bitensky M. W. Isolation of cyclic AMP and cyclic GMP by thin-layer chromatography. Application to assay of adenylate cyclase, guanylate cyclase, and cyclic nucleotide phosphodiesterase. Anal Biochem. 1974 Oct;61(2):336–348. doi: 10.1016/0003-2697(74)90400-x. [DOI] [PubMed] [Google Scholar]
  10. Matuo Y., Wheeler M. A., Bitensky M. W. Small fragments from the A subunit of cholera toxin capable of activating adenylate cyclase. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2654–2658. doi: 10.1073/pnas.73.8.2654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moss J., Manganiello V. C., Vaughan M. Hydrolysis of nicotinamide adenine dinucleotide by choleragen and its A protomer: possible role in the activation of adenylate cyclase. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4424–4427. doi: 10.1073/pnas.73.12.4424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moss J., Osborne J. C., Jr, Fishman P. H., Brewer H. B., Jr, Vaughan M., Brady R. O. Effect of gangliosides and substrate analogues on the hydrolysis of nicotinamide adenine dinucleotide by choleragen. Proc Natl Acad Sci U S A. 1977 Jan;74(1):74–78. doi: 10.1073/pnas.74.1.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moss J., Vaughan M. Mechanism of action of choleragen. Evidence for ADP-ribosyltransferase activity with arginine as an acceptor. J Biol Chem. 1977 Apr 10;252(7):2455–2457. [PubMed] [Google Scholar]
  14. Trepel J. B., Chuang D. M., Neff N. H. Transfer of ADP-ribose from NAD to choleragen: a subunit acts as catalyst and acceptor protein. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5440–5442. doi: 10.1073/pnas.74.12.5440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. van Heyningen S. Activity of covalently cross-linked cholera toxin with the adenylate cyclase of intact and lysed pigeon erythrocytes. Biochem J. 1977 Dec 15;168(3):457–463. doi: 10.1042/bj1680457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. van Heyningen S. Cholera toxin. Biol Rev Camb Philos Soc. 1977 Nov;52(4):509–509. doi: 10.1111/j.1469-185x.1977.tb00858.x. [DOI] [PubMed] [Google Scholar]

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