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. 1968 Sep;109(3):369–374. doi: 10.1042/bj1090369

The influence of external sodium ions on the sodium pump in erythrocytes

R N Priestland 1, R Whittam 1
PMCID: PMC1186831  PMID: 4234831

Abstract

1. A study has been made of the interaction between Na+ and K+ on the adenosine triphosphatase activity of erythrocyte `ghosts', and on the K+ influx and Na+ efflux of intact erythrocytes. The adenosine triphosphatase activity and the ion movements were greater at a low external K+ concentration in the absence of Na+ than they were in the presence of 150mm-Na+. The inhibition by external Na+ of K+ influx had an inhibitory constant of 5–10mm. 2. Activation by K+ of kidney microsomal adenosine triphosphatase was retarded by Na+, and activation by Na+ was retarded by K+. Fragmented erythrocyte membranes behaved similarly. 3. These observations suggest that there is competition between Na+ and K+ at the K+-sensitive site of the membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahmed K., Judah J. D., Scholefield P. G. Interaction of sodium and potassium with a cation-dependent adenosine triphosphatase system from rat brain. Biochim Biophys Acta. 1966 Jul 13;120(3):351–360. doi: 10.1016/0926-6585(66)90302-5. [DOI] [PubMed] [Google Scholar]
  2. Berenblum I., Chain E. An improved method for the colorimetric determination of phosphate. Biochem J. 1938 Feb;32(2):295–298. doi: 10.1042/bj0320295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Epstein F. H., Whittam R. The mode of inhibition by calcium of cell-membrane adenosine-triphosphatase activity. Biochem J. 1966 Apr;99(1):232–238. doi: 10.1042/bj0990232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GLYNN I. M. Sodium and potassium movements in human red cells. J Physiol. 1956 Nov 28;134(2):278–310. doi: 10.1113/jphysiol.1956.sp005643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Garrahan P. J., Glynn I. M. The sensitivity of the sodium pump to external sodium. J Physiol. 1967 Sep;192(1):175–188. doi: 10.1113/jphysiol.1967.sp008295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Green A. L., Taylor C. B. Kinetics of (Na+ + K+)-stimulated ATPase of rabbit kidney microsomes. Biochem Biophys Res Commun. 1964;14:118–123. doi: 10.1016/0006-291x(64)90240-2. [DOI] [PubMed] [Google Scholar]
  7. POST R. L., MERRITT C. R., KINSOLVING C. R., ALBRIGHT C. D. Membrane adenosine triphosphatase as a participant in the active transport of sodium and potassium in the human erythrocyte. J Biol Chem. 1960 Jun;235:1796–1802. [PubMed] [Google Scholar]
  8. Robinson J. D. Kinetic studies on a brain microsomal adenosine triphosphatase. Evidence suggesting conformational changes. Biochemistry. 1967 Oct;6(10):3250–3258. doi: 10.1021/bi00862a034. [DOI] [PubMed] [Google Scholar]
  9. SCHATZMANN H. J. THE ROLE OF NA+ AND K+ IN THE OUABAIN-INHIBITION OF THE NA+ + K+-ACTIVATED MEMBRANE ADENOSINE TRIPHOSPHATASE. Biochim Biophys Acta. 1965 Jan 25;94:89–96. doi: 10.1016/0926-6585(65)90011-7. [DOI] [PubMed] [Google Scholar]
  10. SKOU J. C. The influence of some cations on an adenosine triphosphatase from peripheral nerves. Biochim Biophys Acta. 1957 Feb;23(2):394–401. doi: 10.1016/0006-3002(57)90343-8. [DOI] [PubMed] [Google Scholar]
  11. Sachs J. R., Welt L. G. The concentration dependence of active potassium transport in the human red blood cell. J Clin Invest. 1967 Jan;46(1):65–76. doi: 10.1172/JCI105512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Skou J. C., Hilberg C. The effect of sulphydryl-blocking reagents and of urea on the (Na+ + K+)-activated enzyme system. Biochim Biophys Acta. 1965 Nov 22;110(2):359–369. doi: 10.1016/s0926-6593(65)80043-1. [DOI] [PubMed] [Google Scholar]
  13. TOSTESON D. C. Active transport, genetics, and cellular evolution. Fed Proc. 1963 Jan-Feb;22:19–26. [PubMed] [Google Scholar]
  14. WHITTAM R. The asymmetrical stimulation of a membrane adenosine triphosphatase in relation to active cation transport. Biochem J. 1962 Jul;84:110–118. doi: 10.1042/bj0840110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wheeler K. P., Whittam R. Structural and enzymic aspects of the hydrolysis of adenosine triphosphate by membranes of kidney cortex and erythrocytes. Biochem J. 1964 Nov;93(2):349–363. doi: 10.1042/bj0930349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Whittam R., Ager M. E. Vectorial aspects of adenosine-triphosphatase activity in erythrocyte membranes. Biochem J. 1964 Nov;93(2):337–348. doi: 10.1042/bj0930337. [DOI] [PMC free article] [PubMed] [Google Scholar]

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