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. 1968 Oct;109(4):645–650. doi: 10.1042/bj1090645

The determination of glucosamine and galactosamine in some glycoproteins by radioisotope dilution

E R B Graham 1, A Neuberger 1
PMCID: PMC1186950  PMID: 4176325

Abstract

1. The principle of radioisotope dilution was applied on a semi-micro scale to the determination of glucosamine and galactosamine in some glycoproteins, such as immunoglobulins, a urinary glycoprotein and blood-group-specific substances. 2. The glycoprotein was hydrolysed in the presence of [1-14C]glucosamine or [1-14C]galactosamine or both. The amino sugars were made to react with naphthyl isothiocyanate and the products formed were isolated by the method of Scott (1962). The specific radioactivities determined from liquid-scintillation counting and the extinction at 240mμ or 222mμ were used to calculate the content of amino sugars in the protein analysed. 3. Where the values could be compared with those found by other workers, differences were in general not very great. The advantages of the method are that high concentrations of acid can be employed and undesirable side reactions, which may occur with the free sugars, do not affect the results. A potential source of error of the method is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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