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. 1968 Dec;110(3):589–595. doi: 10.1042/bj1100589

Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping of cysteineless mutants of E. coli K 12

M C Jones-Mortimer 1,*
PMCID: PMC1187390  PMID: 4882981

Abstract

To determine to what extent the biosynthesis of cysteine in Escherichia coli resembles that in Salmonella typhimurium, the following experiments were performed. (1) Mutants of E. coli K 12 deficient in the biosynthesis of cysteine were isolated. (2) These mutants were classified by nutritional and biochemical criteria; some mutants lacked a single enzyme of sulphate reduction, other mutants appeared to lack two or more enzymes. (3) The genetic map predicted from the biochemical data alone is shown to be incorrect, and an alternative map, consistent with the genetic data, is proposed for the cys mutants of E. coli.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRITTEN R. J., McCLURE F. T. The amino acid pool in Escherichia coli. Bacteriol Rev. 1962 Sep;26:292–335. doi: 10.1128/br.26.3.292-335.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DEMEREC M., OHTA N. GENETIC ANALYSES OF SALMONELLA TYPHIMURIUM X ESCHERICHIA COLI HYBRIDS. Proc Natl Acad Sci U S A. 1964 Aug;52:317–323. doi: 10.1073/pnas.52.2.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ELLIS R. J. A RAPID ASSAY FOR SULPHITE REDUCTASE. Biochim Biophys Acta. 1964 May 4;85:335–338. doi: 10.1016/0926-6569(64)90255-x. [DOI] [PubMed] [Google Scholar]
  4. Ellis R. J., Humphries S. K., Pasternak C. A. Repressors of sulphate activation in Escherichia coli. Biochem J. 1964 Jul;92(1):167–172. doi: 10.1042/bj0920167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HAYES W. The kinetics of the mating process in Escherichia coli. J Gen Microbiol. 1957 Feb;16(1):97–119. doi: 10.1099/00221287-16-1-97. [DOI] [PubMed] [Google Scholar]
  6. Jones-Mortimer M. C. Positive control of sulphate reduction in Escherichia coli. The nature of the pleiotropic cysteineless mutants of E. coli K12. Biochem J. 1968 Dec;110(3):597–602. doi: 10.1042/bj1100597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones-Mortimer M. C., Wheldrake J. F., Pasternak C. A. The control of sulphate reduction in Escherichia coli by O-acetyl-L-serine. Biochem J. 1968 Mar;107(1):51–53. doi: 10.1042/bj1070051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEDERBERG J., LEDERBERG E. M. Replica plating and indirect selection of bacterial mutants. J Bacteriol. 1952 Mar;63(3):399–406. doi: 10.1128/jb.63.3.399-406.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MATNEY T. S., ACHENBACH N. E. New uses for membrane filters III. Bacterial mating procedure. J Bacteriol. 1962 Oct;84:874–875. doi: 10.1128/jb.84.4.874-875.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mizobuchi K, Demerec M, Gillespie D H. Cysteine Mutants of Salmonella Typhimurium. Genetics. 1962 Nov;47(11):1617–1627. doi: 10.1093/genetics/47.11.1617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PASTERNAK C. A., ELLIS R. J., JONES-MORTIMER M. C., CRICHTON C. E. THE CONTROL OF SULPHATE REDUCTION IN BACTERIA. Biochem J. 1965 Jul;96:270–275. doi: 10.1042/bj0960270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PASTERNAK C. A. Sulphate activation and its control in Escherichia coli and Bacillus subtilis. Biochem J. 1962 Oct;85:44–49. doi: 10.1042/bj0850044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sanderson K. E. Revised linkage map of Salmonella typhimurium. Bacteriol Rev. 1967 Dec;31(4):354–372. doi: 10.1128/br.31.4.354-372.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. TAYLOR A. L., THOMAN M. S. THE GENETIC MAP OF ESCHERICHIA COLI K-12. Genetics. 1964 Oct;50:659–677. doi: 10.1093/genetics/50.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WHELDRAKE J. F., PASTERNAK C. A. THE CONTROL OF SULPHATE ACTIVATION IN BACTERIA. Biochem J. 1965 Jul;96:276–280. doi: 10.1042/bj0960276. [DOI] [PMC free article] [PubMed] [Google Scholar]

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