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. 1998 Jan;62(1):75–79.

Production of virulence-related proteins by Canadian strains of Streptococcus suis capsular type 2.

M Gottschalk 1, A Lebrun 1, H Wisselink 1, J D Dubreuil 1, H Smith 1, U Vecht 1
PMCID: PMC1189447  PMID: 9442945

Abstract

The production of muramidase-released protein (MRP), extracellular protein factor (EF) and hemolysin (suilysin) by 101 Canadian field strains of Streptococcus suis capsular type 2 is described. Most strains (72%) isolated from diseased pigs were MRP-EF- and only 1 strain was MRP+EF+. This strain was also the only 1 to produce the hemolysin. Thirteen strains (15%) were MRP+ EF- and only 3 strains were MRP* EF-. All the strains isolated from clinically healthy pigs as well as a bovine and 2 human isolates had a MRP-EF- phenotype. In addition, 7 strains (8%) had a MRPS phenotype, which had so far been described for S. suis capsular type 1. In conclusion, most Canadian field isolates of S. suis capsular type 2 tested in this study do not produce the virulence-related proteins described so far for this bacterial pathogen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arends J. P., Zanen H. C. Meningitis caused by Streptococcus suis in humans. Rev Infect Dis. 1988 Jan-Feb;10(1):131–137. doi: 10.1093/clinids/10.1.131. [DOI] [PubMed] [Google Scholar]
  2. Beaudoin M., Harel J., Higgins R., Gottschalk M., Frenette M., MacInnes J. I. Molecular analysis of isolates of Streptococcus suis capsular type 2 by restriction-endonuclease-digested DNA separated on SDS-PAGE and by hybridization with an rDNA probe. J Gen Microbiol. 1992 Dec;138(12):2639–2645. doi: 10.1099/00221287-138-12-2639. [DOI] [PubMed] [Google Scholar]
  3. Cantin M., Harel J., Higgins R., Gottschalk M. Antimicrobial resistance patterns and plasmid profiles of Streptococcus suis isolates. J Vet Diagn Invest. 1992 Apr;4(2):170–174. doi: 10.1177/104063879200400209. [DOI] [PubMed] [Google Scholar]
  4. Feder I., Chengappa M. M., Fenwick B., Rider M., Staats J. Partial characterization of Streptococcus suis type 2 hemolysin. J Clin Microbiol. 1994 May;32(5):1256–1260. doi: 10.1128/jcm.32.5.1256-1260.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Galina L., Vecht U., Wisselink H. J., Pijoan C. Prevalence of various phenotypes of Streptococcus suis isolated from swine in the U.S.A. based on the presence of muraminidase-released protein and extracellular factor. Can J Vet Res. 1996 Jan;60(1):72–74. [PMC free article] [PubMed] [Google Scholar]
  6. Gottschalk M. G., Lacouture S., Dubreuil J. D. Characterization of Streptococcus suis capsular type 2 haemolysin. Microbiology. 1995 Jan;141(Pt 1):189–195. doi: 10.1099/00221287-141-1-189. [DOI] [PubMed] [Google Scholar]
  7. Gottschalk M., Higgins R., Jacques M., Dubreuil D. Production and characterization of two Streptococcus suis capsular type 2 mutants. Vet Microbiol. 1992 Jan;30(1):59–71. doi: 10.1016/0378-1135(92)90094-a. [DOI] [PubMed] [Google Scholar]
  8. Higgins R., Gottschalk M. An update on Streptococcus suis identification. J Vet Diagn Invest. 1990 Jul;2(3):249–252. doi: 10.1177/104063879000200324. [DOI] [PubMed] [Google Scholar]
  9. Higgins R., Gottschalk M., Beaudoin M., Rawluk S. A. Distribution of Streptococcus suis capsular types in Quebec and western Canada. Can Vet J. 1992 Jan;33(1):27–30. [PMC free article] [PubMed] [Google Scholar]
  10. Higgins R., Gottschalk M., Boudreau M., Lebrun A., Henrichsen J. Description of six new capsular types (29-34) of Streptococcus suis. J Vet Diagn Invest. 1995 Jul;7(3):405–406. doi: 10.1177/104063879500700322. [DOI] [PubMed] [Google Scholar]
  11. Holt M. E., Enright M. R., Alexander T. J. Protective effect of sera raised against different fractions of Streptococcus suis type 2. J Comp Pathol. 1990 Jul;103(1):85–94. doi: 10.1016/s0021-9975(08)80138-7. [DOI] [PubMed] [Google Scholar]
  12. Jacobs A. A., Loeffen P. L., van den Berg A. J., Storm P. K. Identification, purification, and characterization of a thiol-activated hemolysin (suilysin) of Streptococcus suis. Infect Immun. 1994 May;62(5):1742–1748. doi: 10.1093/benz/9780199773787.article.b00034458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jacobs A. A., van den Berg A. J., Baars J. C., Nielsen B., Johannsen L. W. Production of suilysin, the thiol-activated haemolysin of Streptococcus suis, by field isolates from diseased pigs. Vet Rec. 1995 Sep 16;137(12):295–296. doi: 10.1136/vr.137.12.295. [DOI] [PubMed] [Google Scholar]
  14. Mwaniki C. G., Robertson I. D., Trott D. J., Atyeo R. F., Lee B. J., Hampson D. J. Clonal analysis and virulence of Australian isolates of Streptococcus suis type 2. Epidemiol Infect. 1994 Oct;113(2):321–334. doi: 10.1017/s095026880005175x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Quessy S., Dubreuil J. D., Caya M., Higgins R. Discrimination of virulent and avirulent Streptococcus suis capsular type 2 isolates from different geographical origins. Infect Immun. 1995 May;63(5):1975–1979. doi: 10.1128/iai.63.5.1975-1979.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Trottier S., Higgins R., Brochu G., Gottschalk M. A case of human endocarditis due to Streptococcus suis in North America. Rev Infect Dis. 1991 Nov-Dec;13(6):1251–1252. doi: 10.1093/clinids/13.6.1251. [DOI] [PubMed] [Google Scholar]
  17. Vecht U., Arends J. P., van der Molen E. J., van Leengoed L. A. Differences in virulence between two strains of Streptococcus suis type II after experimentally induced infection of newborn germ-free pigs. Am J Vet Res. 1989 Jul;50(7):1037–1043. [PubMed] [Google Scholar]
  18. Vecht U., Wisselink H. J., Anakotta J., Smith H. E. Discrimination between virulent and nonvirulent Streptococcus suis type 2 strains by enzyme-linked immunosorbent assay. Vet Microbiol. 1993 Jan;34(1):71–82. doi: 10.1016/0378-1135(93)90008-u. [DOI] [PubMed] [Google Scholar]
  19. Vecht U., Wisselink H. J., Jellema M. L., Smith H. E. Identification of two proteins associated with virulence of Streptococcus suis type 2. Infect Immun. 1991 Sep;59(9):3156–3162. doi: 10.1128/iai.59.9.3156-3162.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vecht U., Wisselink H. J., van Dijk J. E., Smith H. E. Virulence of Streptococcus suis type 2 strains in newborn germfree pigs depends on phenotype. Infect Immun. 1992 Feb;60(2):550–556. doi: 10.1128/iai.60.2.550-556.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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