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. 1999 Apr;63(2):142–147.

Plasma myeloperoxidase level and polymorphonuclear leukocyte activation in horses suffering from large intestinal obstruction requiring surgery: preliminary results.

S Grulke 1, H Benbarek 1, I Caudron 1, G Deby-Dupont 1, M Mathy-Hartert 1, F Farnir 1, C Deby 1, M Lamy 1, D Serteyn 1
PMCID: PMC1189534  PMID: 10369573

Abstract

Myeloperoxidase (MPO) is a specific enzyme of neutrophil azurophilic granules with a strong oxidative activity. Thanks to a radioimmunoassay of equine myeloperoxidase, the authors have observed a significantly higher plasma level of MPO in horses operated for strangulation obstruction of the large intestine (n = 6) than in horses suffering from a non-strangulating displacement of the large intestine (n = 9). For the 2 groups, 3 phases were distinguished: reception (P1), intensive care (P2) and terminal phase (P3). The mean peak values of MPO for these phases were 121.6 ng/mL (P1), 168.6 ng/mL (P2), and 107.0 ng/mL (P3) for the non-strangulating group, and 242.6 ng/mL (P1); 426.0 ng/mL (P2), and 379.5 ng/mL (P3) for the strangulation group. The variations of the mean peak values of plasma MPO were significantly different between the 2 groups and between the different phases. A significant increase of the least square means of MPO was observed between P1 and P2. A significant decrease of the least square means of the number of circulating leukocytes was observed between P1 and P3. Polymorphonuclear neutrophil activation could play a major role in the pathogenesis of acute abdominal disease and endotoxic shock.

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Selected References

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  1. Barclay W. P., Foerner J. J., Phillips T. N. Volvulus of the large colon in the horse. J Am Vet Med Assoc. 1980 Oct 1;177(7):629–630. [PubMed] [Google Scholar]
  2. Benbarek H., Deby-Dupont G., Caudron I., Grülke S., Deby C., Lamy M., Serteyn D. Interactions between lipopolysaccharides and blood factors on the stimulation of equine polymorphonuclear neutrophils. Vet Immunol Immunopathol. 1998 Aug 31;64(4):313–322. doi: 10.1016/s0165-2427(98)00142-1. [DOI] [PubMed] [Google Scholar]
  3. Carreras M. C., Pargament G. A., Catz S. D., Poderoso J. J., Boveris A. Kinetics of nitric oxide and hydrogen peroxide production and formation of peroxynitrite during the respiratory burst of human neutrophils. FEBS Lett. 1994 Mar 14;341(1):65–68. doi: 10.1016/0014-5793(94)80241-6. [DOI] [PubMed] [Google Scholar]
  4. Faymonville M. E., Pincemail J., Duchateau J., Paulus J. M., Adam A., Deby-Dupont G., Deby C., Albert A., Larbuisson R., Limet R. Myeloperoxidase and elastase as markers of leukocyte activation during cardiopulmonary bypass in humans. J Thorac Cardiovasc Surg. 1991 Aug;102(2):309–317. [PubMed] [Google Scholar]
  5. Harrison I. W. Equine large intestinal volvulus. A review of 124 cases. Vet Surg. 1988 Mar-Apr;17(2):77–81. doi: 10.1111/j.1532-950x.1988.tb00281.x. [DOI] [PubMed] [Google Scholar]
  6. Horne M. M., Pascoe P. J., Ducharme N. G., Barker I. K., Grovum W. L. Attempts to modify reperfusion injury of equine jejunal mucosa using dimethylsulfoxide, allopurinol, and intraluminal oxygen. Vet Surg. 1994 Jul-Aug;23(4):241–249. doi: 10.1111/j.1532-950x.1994.tb00478.x. [DOI] [PubMed] [Google Scholar]
  7. King C. C., Jefferson M. M., Thomas E. L. Secretion and inactivation of myeloperoxidase by isolated neutrophils. J Leukoc Biol. 1997 Mar;61(3):293–302. doi: 10.1002/jlb.61.3.293. [DOI] [PubMed] [Google Scholar]
  8. Klebanoff S. J., Hamon C. B. Role of myeloperoxidase-mediated antimicrobial systems in intact leukocytes. J Reticuloendothel Soc. 1972 Aug;12(2):170–196. [PubMed] [Google Scholar]
  9. Koppenol W. H., Moreno J. J., Pryor W. A., Ischiropoulos H., Beckman J. S. Peroxynitrite, a cloaked oxidant formed by nitric oxide and superoxide. Chem Res Toxicol. 1992 Nov-Dec;5(6):834–842. doi: 10.1021/tx00030a017. [DOI] [PubMed] [Google Scholar]
  10. Krawisz J. E., Sharon P., Stenson W. F. Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models. Gastroenterology. 1984 Dec;87(6):1344–1350. [PubMed] [Google Scholar]
  11. Larrick J. W., Kunkel S. L. The role of tumor necrosis factor and interleukin 1 in the immunoinflammatory response. Pharm Res. 1988 Mar;5(3):129–139. doi: 10.1023/a:1015904721223. [DOI] [PubMed] [Google Scholar]
  12. MacDonald M. H., Pascoe J. R., Stover S. M., Meagher D. M. Survival after small intestine resection and anastomosis in horses. Vet Surg. 1989 Nov-Dec;18(6):415–423. doi: 10.1111/j.1532-950x.1990.tb01116.x. [DOI] [PubMed] [Google Scholar]
  13. Mathy-Hartert M., Bourgeois E., Grülke S., Deby-Dupont G., Caudron I., Deby C., Lamy M., Serteyn D. Purification of myeloperoxidase from equine polymorphonuclear leucocytes. Can J Vet Res. 1998 Apr;62(2):127–132. [PMC free article] [PubMed] [Google Scholar]
  14. Moore R. M., Bertone A. L., Bailey M. Q., Muir W. W., Beard W. L. Neutrophil accumulation in the large colon of horses during low-flow ischemia and reperfusion. Am J Vet Res. 1994 Oct;55(10):1454–1463. [PubMed] [Google Scholar]
  15. Moore R. M., Muir W. W., Granger D. N. Mechanisms of gastrointestinal ischemia-reperfusion injury and potential therapeutic interventions: a review and its implications in the horse. J Vet Intern Med. 1995 May-Jun;9(3):115–132. doi: 10.1111/j.1939-1676.1995.tb03285.x. [DOI] [PubMed] [Google Scholar]
  16. Morris D. D. Endotoxemia in horses. A review of cellular and humoral mediators involved in its pathogenesis. J Vet Intern Med. 1991 May-Jun;5(3):167–181. doi: 10.1111/j.1939-1676.1991.tb00944.x. [DOI] [PubMed] [Google Scholar]
  17. Pascoe P. J., McDonell W. N., Trim C. M., Van Gorder J. Mortality rates and associated factors in equine colic operations - a retrospective study of 341 operations. Can Vet J. 1983 Mar;24(3):76–85. [PMC free article] [PubMed] [Google Scholar]
  18. Pincemail J., Deby-Dupont G., Deby C., Thirion A., Torpier G., Faymonville M. E., Damas P., Tomassini M., Lamy M., Franchimont P. Fast double antibody radioimmunoassay of human granulocyte myeloperoxidase and its application to plasma. J Immunol Methods. 1991 Mar 21;137(2):181–191. doi: 10.1016/0022-1759(91)90023-9. [DOI] [PubMed] [Google Scholar]
  19. Seethanathan P., Bottoms G. D., Schafer K. Characterization of release of tumor necrosis factor, interleukin-1, and superoxide anion from equine white blood cells in response to endotoxin. Am J Vet Res. 1990 Aug;51(8):1221–1225. [PubMed] [Google Scholar]
  20. Shandall A. A., Williams G. T., Hallett M. B., Young H. L. Colonic healing: a role for polymorphonuclear leucocytes and oxygen radical production. Br J Surg. 1986 Mar;73(3):225–228. doi: 10.1002/bjs.1800730325. [DOI] [PubMed] [Google Scholar]
  21. Snyder J. R., Olander H. J., Pascoe J. R., Holland M., Kurpershoek C. J. Morphologic alterations observed during experimental ischemia of the equine large colon. Am J Vet Res. 1988 Jun;49(6):801–809. [PubMed] [Google Scholar]
  22. Snyder J. R., Pascoe J. R., Olander H. J., Harmon F. A., Hinds D. M., Vatistasa N. J., Tyler W. S. Ultrastructural mucosal injury after experimental ischemia of the ascending colon in horses. Am J Vet Res. 1992 Oct;53(10):1917–1924. [PubMed] [Google Scholar]
  23. Snyder J. R., Pascoe J. R., Olander H. J., Spier S. J., Meagher D. M., Bleifer D. R. Strangulating volvulus of the ascending colon in horses. J Am Vet Med Assoc. 1989 Sep 15;195(6):757–764. [PubMed] [Google Scholar]
  24. Van der Vliet A., Smith D., O'Neill C. A., Kaur H., Darley-Usmar V., Cross C. E., Halliwell B. Interactions of peroxynitrite with human plasma and its constituents: oxidative damage and antioxidant depletion. Biochem J. 1994 Oct 1;303(Pt 1):295–301. doi: 10.1042/bj3030295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weiland J. E., Davis W. B., Holter J. F., Mohammed J. R., Dorinsky P. M., Gadek J. E. Lung neutrophils in the adult respiratory distress syndrome. Clinical and pathophysiologic significance. Am Rev Respir Dis. 1986 Feb;133(2):218–225. doi: 10.1164/arrd.1986.133.2.218. [DOI] [PubMed] [Google Scholar]
  26. Weiss S. J., Lampert M. B., Test S. T. Long-lived oxidants generated by human neutrophils: characterization and bioactivity. Science. 1983 Nov 11;222(4624):625–628. doi: 10.1126/science.6635660. [DOI] [PubMed] [Google Scholar]
  27. White N. A., Moore J. N., Trim C. M. Mucosal alterations in experimentally induced small intestinal strangulation obstruction in ponies. Am J Vet Res. 1980 Feb;41(2):193–198. [PubMed] [Google Scholar]
  28. Winterbourn C. C., van den Berg J. J., Roitman E., Kuypers F. A. Chlorohydrin formation from unsaturated fatty acids reacted with hypochlorous acid. Arch Biochem Biophys. 1992 Aug 1;296(2):547–555. doi: 10.1016/0003-9861(92)90609-z. [DOI] [PubMed] [Google Scholar]

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