Abstract
This study was designed to determine if a single 0.5 microg administration of recombinant murine interleukin-12 (IL-12) would influence immune responses of mice vaccinated with live or killed Brucella abortus strain RB51 (SRB51). Mice were vaccinated intraperitoneally with 5 x 10(8) cfu of live or gamma-irradiated SRB51 bacteria alone, or in combination with 0.5 microg of IL-12. Control mice received saline or 0.5 microg of IL-12. Serologic responses and spleen weights after vaccination were greater in mice vaccinated with live SRB51 when compared to mice receiving killed SRB51 or control treatments. Administration of a single dose of IL-12 as a vaccine adjuvant did not influence immune responses, clearance of live SRB51, or resistance against B. abortus strain 2308 (S2308) challenge. The results of this study suggest that a single administration of 0.5 microg of IL-12 at the time of vaccination does not have significant adjuvant effects on vaccine-induced immune responses against live or killed Brucella.
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Selected References
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- Afonso L. C., Scharton T. M., Vieira L. Q., Wysocka M., Trinchieri G., Scott P. The adjuvant effect of interleukin-12 in a vaccine against Leishmania major. Science. 1994 Jan 14;263(5144):235–237. doi: 10.1126/science.7904381. [DOI] [PubMed] [Google Scholar]
- Brunda M. J. Interleukin-12. J Leukoc Biol. 1994 Feb;55(2):280–288. doi: 10.1002/jlb.55.2.280. [DOI] [PubMed] [Google Scholar]
- Cheville N. F., Stevens M. G., Jensen A. E., Tatum F. M., Halling S. M. Immune responses and protection against infection and abortion in cattle experimentally vaccinated with mutant strains of Brucella abortus. Am J Vet Res. 1993 Oct;54(10):1591–1597. [PubMed] [Google Scholar]
- Manetti R., Parronchi P., Giudizi M. G., Piccinni M. P., Maggi E., Trinchieri G., Romagnani S. Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells. J Exp Med. 1993 Apr 1;177(4):1199–1204. doi: 10.1084/jem.177.4.1199. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller M. A., Skeen M. J., Ziegler H. K. Protective immunity to Listeria monocytogenes elicited by immunization with heat-killed Listeria and IL-12. Potential mechanism of IL-12 adjuvanticity. Ann N Y Acad Sci. 1996 Oct 25;797:207–227. doi: 10.1111/j.1749-6632.1996.tb52962.x. [DOI] [PubMed] [Google Scholar]
- Stevens M. G., Olsen S. C., Pugh G. W., Jr, Brees D. Comparison of immune responses and resistance to brucellosis in mice vaccinated with Brucella abortus 19 or RB51. Infect Immun. 1995 Jan;63(1):264–270. doi: 10.1128/iai.63.1.264-270.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zaitseva M., Golding H., Manischewitz J., Webb D., Golding B. Brucella abortus as a potential vaccine candidate: induction of interleukin-12 secretion and enhanced B7.1 and B7.2 and intercellular adhesion molecule 1 surface expression in elutriated human monocytes stimulated by heat-inactivated B. abortus. Infect Immun. 1996 Aug;64(8):3109–3117. doi: 10.1128/iai.64.8.3109-3117.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zhan Y., Liu Z., Cheers C. Tumor necrosis factor alpha and interleukin-12 contribute to resistance to the intracellular bacterium Brucella abortus by different mechanisms. Infect Immun. 1996 Jul;64(7):2782–2786. doi: 10.1128/iai.64.7.2782-2786.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]