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. 1992 Apr;68(2):111–116. doi: 10.1136/sti.68.2.111

Antimicrobial susceptibility of Neisseria gonorrhoeae in Zaire: high level plasmid-mediated tetracycline resistance in central Africa.

E Van Dyck 1, R Rossau 1, M Duhamel 1, F Behets 1, M Laga 1, M Nzila 1, S Bygdeman 1, H Van Heuverswijn 1, P Piot 1
PMCID: PMC1194822  PMID: 1582653

Abstract

OBJECTIVE--To determine the in vitro antimicrobial susceptibility of gonococcal strains isolated in 1988 among female prostitutes in Kinshasa, Zaire and to characterise strains with high level tetracycline resistance. METHODS--Minimal inhibitory concentrations of 8 antimicrobials were measured by agar dilution technique. Plasmid-profiles and serovars were determined. RESULTS--Two hundred and thirteen strains of Neisseria gonorrhoeae were tested of which 59% were beta-lactamase producers and an additional 21% showed intermediate or chromosomal resistance to penicillin (MIC = 0.5-8 mg/l). Eleven percent of the strains were resistant to the combination sulfamethoxazole-trimethoprim (MIC greater than 8 mg/l) and 57% of the isolates showed decreased susceptibility to thiamphenicol (MIC = 1-4 mg/l). All strains were sensitive to spectinomycin, norfloxacin and ceftriaxone and moderately sensitive to kanamycin. Chromosomal resistance to tetracycline was observed in 45% of strains (MIC = 2-8 mg/l). Ten percent were highly resistant to tetracycline (TRNG, MIC = 16-128 mg/l) and were shown to carry a plasmid borne Tet M determinant; such strains were not found in Kinshasa in 1985. TRNG belonged to 4 different serovars, which were also the dominant serovars in non-TRNG. CONCLUSION--These findings illustrate the high frequency of multiresistant gonococci in Zaire and suggest that high level tetracycline resistant strains of N. gonorrhoeae have become endemic in Central Africa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bogaerts J., Vandepitte J., Van Dyck E., Vanhoof R., Dekegel M., Piot P. In vitro antimicrobial sensitivity of Neisseria gonorrhoeae from Rwanda. Genitourin Med. 1986 Aug;62(4):217–220. doi: 10.1136/sti.62.4.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Casin I., Perenet F., Issoire C., Riou J. Y., Morel P., Perol Y. High-level tetracycline resistance in penicillinase-producing Neisseria gonorrhoeae in France. Eur J Clin Microbiol Infect Dis. 1989 Oct;8(10):929–931. doi: 10.1007/BF01963788. [DOI] [PubMed] [Google Scholar]
  3. D'Costa L. J., Plummer F. A., Bowmer I., Fransen L., Piot P., Ronald A. R., Nsanze H. Prostitutes are a major reservoir of sexually transmitted diseases in Nairobi, Kenya. Sex Transm Dis. 1985 Apr-Jun;12(2):64–67. doi: 10.1097/00007435-198504000-00002. [DOI] [PubMed] [Google Scholar]
  4. Gascoyne D. M., Heritage J., Hawkey P. M. The 25.2 MDa tetracycline-resistance plasmid is not derived from the 24.5 MDa conjugative plasmid of Neisseria gonorrhoeae. J Antimicrob Chemother. 1990 Jan;25(1):39–47. doi: 10.1093/jac/25.1.39. [DOI] [PubMed] [Google Scholar]
  5. Ison C. A., Terry P., Bendayna K., Gill M. J., Adams J., Woodford N. Tetracycline-resistant gonococci in UK. Lancet. 1988 Mar 19;1(8586):651–652. doi: 10.1016/s0140-6736(88)91453-5. [DOI] [PubMed] [Google Scholar]
  6. Knapp J. S., Zenilman J. M., Biddle J. W., Perkins G. H., DeWitt W. E., Thomas M. L., Johnson S. R., Morse S. A. Frequency and distribution in the United States of strains of Neisseria gonorrhoeae with plasmid-mediated, high-level resistance to tetracycline. J Infect Dis. 1987 Apr;155(4):819–822. doi: 10.1093/infdis/155.4.819. [DOI] [PubMed] [Google Scholar]
  7. Laga M., Plummer F. A., Piot P., Datta P., Namaara W., Ndinya-Achola J. O., Nzanze H., Maitha G., Ronald A. R., Pamba H. O. Prophylaxis of gonococcal and chlamydial ophthalmia neonatorum. A comparison of silver nitrate and tetracycline. N Engl J Med. 1988 Mar 17;318(11):653–657. doi: 10.1056/NEJM198803173181101. [DOI] [PubMed] [Google Scholar]
  8. Martin P., Trieu-Cuot P., Courvalin P. Nucleotide sequence of the tetM tetracycline resistance determinant of the streptococcal conjugative shuttle transposon Tn1545. Nucleic Acids Res. 1986 Sep 11;14(17):7047–7058. doi: 10.1093/nar/14.17.7047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Meheus A., De Clercq A., Prat R. Prevalence of gonorrhoea in prostitutes in a Central African town. Br J Vener Dis. 1974 Feb;50(1):50–52. doi: 10.1136/sti.50.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Morse S. A., Johnson S. R., Biddle J. W., Roberts M. C. High-level tetracycline resistance in Neisseria gonorrhoeae is result of acquisition of streptococcal tetM determinant. Antimicrob Agents Chemother. 1986 Nov;30(5):664–670. doi: 10.1128/aac.30.5.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nsanze H., D'Costa L. J., Owili D. M., Ilako F., Ndinya-Achola, Piot P. Treatment of gonorrhea with single-dose thiamphenicol in Kenya. Sex Transm Dis. 1984 Oct-Dec;11(4 Suppl):376–378. doi: 10.1097/00007435-198410001-00015. [DOI] [PubMed] [Google Scholar]
  12. Osoba A. O., Johnston N. A., Ogunbanjo B. O., Ochei J. Plasmid profile of Neisseria gonorrhoeae in Nigeria and efficacy of spectinomycin in treating gonorrhoea. Genitourin Med. 1987 Feb;63(1):1–5. doi: 10.1136/sti.63.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Roberts M. C., Knapp J. S. Host range of the conjugative 25.2-megadalton tetracycline resistance plasmid from Neisseria gonorrhoeae and related species. Antimicrob Agents Chemother. 1988 Apr;32(4):488–491. doi: 10.1128/aac.32.4.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roberts M. C., Wagenvoort J. H., van Klingeren B., Knapp J. S. tetM- and beta-lactamase-containing Neisseria gonorrhoeae (tetracycline resistant and penicillinase producing) in The Netherlands. Antimicrob Agents Chemother. 1988 Jan;32(1):158–158. doi: 10.1128/aac.32.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rossau R., Vanmechelen E., De Ley J., Van Heuverswijn H. Specific Neisseria gonorrhoeae DNA-probes derived from ribosomal RNA. J Gen Microbiol. 1989 Jun;135(6):1735–1745. doi: 10.1099/00221287-135-6-1735. [DOI] [PubMed] [Google Scholar]
  16. Tam M. R., Buchanan T. M., Sandström E. G., Holmes K. K., Knapp J. S., Siadak A. W., Nowinski R. C. Serological classification of Neisseria gonorrhoeae with monoclonal antibodies. Infect Immun. 1982 Jun;36(3):1042–1053. doi: 10.1128/iai.36.3.1042-1053.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Van de Velden L., Van Dyck E., De Schampheleire I., Dankaratou Y., Piot P. Neisseria gonorrhoea à Pikine (Sénégal): surveillance de la sensibilité aux antibiotiques. Ann Soc Belg Med Trop. 1990 Jun;70(2):99–103. [PubMed] [Google Scholar]
  18. van Klingeren B., Dessens-Kroon M., Verheuvel M. Increased tetracycline resistance in gonococci in The Netherlands. Lancet. 1989 Nov 25;2(8674):1278–1278. doi: 10.1016/s0140-6736(89)91884-9. [DOI] [PubMed] [Google Scholar]

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