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. 1983 Aug;341:507–515. doi: 10.1113/jphysiol.1983.sp014820

The effect of naloxone on vasopressin release from rat neurohypophysis incubated in vitro.

W Knepel, D K Meyer
PMCID: PMC1195347  PMID: 6620188

Abstract

Rat posterior pituitaries were superfused in vitro and stimulated electrically. The concentrations of vasopressin in the superfusion medium were determined by radioimmunoassay. When the pulses were applied in 10 sec trains with 10 sec intervals, vasopressin release per pulse increased progressively over the frequency range of 3-12 pulses/sec applied within the trains. The release was blocked by addition of tetrodotoxin or by removal of calcium ions from the superfusion medium. The opiate antagonist naloxone 1 or 10 microM was introduced into the superfusion medium before a second period of stimulation and enhanced vasopressin release from neurointermediate lobes after phasic stimulation at 9 pulses/sec within the trains, when compared to controls. However, naloxone 10 microM had not effect on vasopressin release from isolated neural lobes (intermediate lobes removed), although the addition of camel beta-endorphin 2 microM inhibited vasopressin release in a naloxone-reversible manner. After continuous stimulation at a frequency of 13 Hz naloxone 10 microM did not influence the release of vasopressin from neurointermediate lobes. We conclude that the evoked release of vasopressin from the neurointermediate lobe is reduced by an endogenous opiate of intermediate lobe origin, possibly beta-endorphin. Appropriate stimulation conditions are necessary for this mechanism to function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anhut H., Knepel W., Nutto D., Hertting G. Vasopressin stimulates release of beta-lipotropin and beta-endorphin in conscious rats as measured by radioimmunoassay of unextracted plasma. Naunyn Schmiedebergs Arch Pharmacol. 1981 Feb;316(1):59–63. doi: 10.1007/BF00507229. [DOI] [PubMed] [Google Scholar]
  2. Aziz L. A., Forsling M. L., Woolf C. J. The effect of intracerebroventricular injections of morphine on vasopressin release in the rat. J Physiol. 1981 Feb;311:401–409. doi: 10.1113/jphysiol.1981.sp013592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bergland R. M., Page R. B. Can the pituitary secrete directly to the brain? (Affirmative anatomical evidence). Endocrinology. 1978 May;102(5):1325–1338. doi: 10.1210/endo-102-5-1325. [DOI] [PubMed] [Google Scholar]
  4. Bergland R. M., Page R. B. Pituitary-brain vascular relations: a new paradigm. Science. 1979 Apr 6;204(4388):18–24. doi: 10.1126/science.373118. [DOI] [PubMed] [Google Scholar]
  5. Bicknell R. J., Leng G. Endogenous opiates regulate oxytocin but not vasopressin secretion from the neurohypophysis. Nature. 1982 Jul 8;298(5870):161–162. doi: 10.1038/298161a0. [DOI] [PubMed] [Google Scholar]
  6. Bicknell R. J., Leng G. Relative efficiency of neural firing patterns for vasopressin release in vitro. Neuroendocrinology. 1981 Nov;33(5):295–299. doi: 10.1159/000123248. [DOI] [PubMed] [Google Scholar]
  7. Bisset G. W., Chowdrey H. S., Feldberg W. Release of vasopressin by enkephalin. Br J Pharmacol. 1978 Mar;62(3):370–371. doi: 10.1111/j.1476-5381.1978.tb08472.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bower A., Hadley M. E., Hruby V. J. Biogenic amines and control of melanophore stimulating hormone release. Science. 1974 Apr 5;184(4132):70–72. doi: 10.1126/science.184.4132.70. [DOI] [PubMed] [Google Scholar]
  9. Clement-Jones V., Lowry P. J., Rees L. H., Besser G. M. Met-enkephalin circulates in human plasma. Nature. 1980 Jan 17;283(5744):295–297. doi: 10.1038/283295a0. [DOI] [PubMed] [Google Scholar]
  10. Dutton A., Dyball R. E. Phasic firing enhances vasopressin release from the rat neurohypophysis. J Physiol. 1979 May;290(2):433–440. doi: 10.1113/jphysiol.1979.sp012781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grossman A., Besser G. M., Milles J. J., Baylis P. H. Inhibition of vasopressin release in man by an opiate peptide. Lancet. 1980 Nov 22;2(8204):1108–1110. doi: 10.1016/s0140-6736(80)92542-8. [DOI] [PubMed] [Google Scholar]
  12. Guillemin R., Vargo T., Rossier J., Minick S., Ling N., Rivier C., Vale W., Bloom F. beta-Endorphin and adrenocorticotropin are selected concomitantly by the pituitary gland. Science. 1977 Sep 30;197(4311):1367–1369. doi: 10.1126/science.197601. [DOI] [PubMed] [Google Scholar]
  13. Iversen L. L., Iversen S. D., Bloom F. E. Opiate receptors influence vasopressin release from nerve terminals in rat neurohypophysis. Nature. 1980 Mar 27;284(5754):350–351. doi: 10.1038/284350a0. [DOI] [PubMed] [Google Scholar]
  14. Knepel W., Meyer D. K. Effect of isoprenaline and trimethidinium on the plasma vasopressin concentration in conscious rats. Arch Int Pharmacodyn Ther. 1980 Jun;245(2):249–261. [PubMed] [Google Scholar]
  15. Knepel W., Nutto D., Anhut H., Hertting G. Naloxone promotes stimulus-evoked vasopressin release in vivo. Eur J Pharmacol. 1980 Aug 8;65(4):449–450. doi: 10.1016/0014-2999(80)90353-2. [DOI] [PubMed] [Google Scholar]
  16. Knepel W., Nutto D., Anhut H., Hertting G. Vasopressin and beta-endorphin release after osmotic and non-osmotic stimuli: effect of naloxone and dexamethasone. Eur J Pharmacol. 1982 Feb 5;77(4):299–306. doi: 10.1016/0014-2999(82)90132-7. [DOI] [PubMed] [Google Scholar]
  17. Knepel W., Nutto D., Hertting G. Evidence for inhibition by beta-endorphin of vasopressin release during foot shock-induced stress in the rat. Neuroendocrinology. 1982;34(5):353–356. doi: 10.1159/000123327. [DOI] [PubMed] [Google Scholar]
  18. Knepel W., Reimann W. Inhibition by morphine and beta-endorphin of vasopressin release evoked by electrical stimulation of the rat medial basal hypothalamus in vitro. Brain Res. 1982 Apr 29;238(2):484–488. doi: 10.1016/0006-8993(82)90128-7. [DOI] [PubMed] [Google Scholar]
  19. Knepel W., Reimann W., Nutto D. On the mechanism of the vasopressin-induced inhibition of renin release. Horm Metab Res. 1982 Mar;14(3):157–160. doi: 10.1055/s-2007-1018953. [DOI] [PubMed] [Google Scholar]
  20. Lightman S. L., Forsling M. L. Evidence for endogenous opioid control of vasopressin release in man. J Clin Endocrinol Metab. 1980 Mar;50(3):569–571. doi: 10.1210/jcem-50-3-569. [DOI] [PubMed] [Google Scholar]
  21. Lightman S. L., Iversen L. L., Forsling M. L. Dopamine and [D-ALA2, D-Leu5]enkephalin inhibit the electrically stimulated neurohypophyseal release of vasopressin in vitro: evidence for calcium-dependent opiate action. J Neurosci. 1982 Jan;2(1):78–81. doi: 10.1523/JNEUROSCI.02-01-00078.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lutz-Bucher B., Koch B. Evidence for a direct inhibitory effect of morphine on the secretion of posterior pituitary hormones. Eur J Pharmacol. 1980 Sep 5;66(4):375–378. doi: 10.1016/0014-2999(80)90470-7. [DOI] [PubMed] [Google Scholar]
  23. Millan M. J., Przewłocki R., Jerlicz M., Gramsch C., Höllt V., Herz A. Stress-induced release of brain and pituitary beta-endorphin: major role of endorphins in generation of hyperthermia, not analgesia. Brain Res. 1981 Mar 16;208(2):325–338. doi: 10.1016/0006-8993(81)90561-8. [DOI] [PubMed] [Google Scholar]
  24. NARAHASHI T., MOORE J. W., SCOTT W. R. TETRODOTOXIN BLOCKAGE OF SODIUM CONDUCTANCE INCREASE IN LOBSTER GIANT AXONS. J Gen Physiol. 1964 May;47:965–974. doi: 10.1085/jgp.47.5.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Nordmann J. J., Dyball R. E. Effects of veratridine on Ca fluxes and the release of oxytocin and vasopressin from the isolated rat neurohypophysis. J Gen Physiol. 1978 Sep;72(3):297–304. doi: 10.1085/jgp.72.3.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Oliver C., Mical R. S., Porter J. C. Hypothalamic-pituitary vasculature: evidence for retrograde blood flow in the pituitary stalk. Endocrinology. 1977 Aug;101(2):598–604. doi: 10.1210/endo-101-2-598. [DOI] [PubMed] [Google Scholar]
  27. Pelletier G., Leclerc R., Labrie F., Cote J., Chretien M., Lis M. Immunohistochemical localization of beta-lipotropic hormone in the pituitary gland. Endocrinology. 1977 Mar;100(3):770–776. doi: 10.1210/endo-100-3-770. [DOI] [PubMed] [Google Scholar]
  28. Poulain D. A., Wakerley J. B. Electrophysiology of hypothalamic magnocellular neurones secreting oxytocin and vasopressin. Neuroscience. 1982 Apr;7(4):773–808. doi: 10.1016/0306-4522(82)90044-6. [DOI] [PubMed] [Google Scholar]
  29. Przewłocki R., Millan M. J., Gramsch C., Millan M. H., Herz A. The influence of selective adeno- and neurointermedio-hypophysectomy upon plasma and brain levels of beta-endorphin and their response to stress in rats. Brain Res. 1982 Jun 17;242(1):107–117. doi: 10.1016/0006-8993(82)90500-5. [DOI] [PubMed] [Google Scholar]
  30. Rossier J., Battenberg E., Pittman Q., Bayon A., Koda L., Miller R., Guillemin R., Bloom F. Hypothalamic enkephalin neurones may regulate the neurohypophysis. Nature. 1979 Feb 22;277(5698):653–655. doi: 10.1038/277653a0. [DOI] [PubMed] [Google Scholar]
  31. Seizinger B. R., Höllt V. In vitro biosynthesis and N-acetylation of beta-endorphin in pars intermedia of the rat pituitary. Biochem Biophys Res Commun. 1980 Sep 30;96(2):535–543. doi: 10.1016/0006-291x(80)91389-3. [DOI] [PubMed] [Google Scholar]
  32. Simantov R., Snyder S. H. Opiate receptor binding in the pituitary gland. Brain Res. 1977 Mar 18;124(1):178–184. doi: 10.1016/0006-8993(77)90877-0. [DOI] [PubMed] [Google Scholar]
  33. Smyth D. G., Zakarian S. Selective processing of beta-endorphin in regions of porcine pituitary. Nature. 1980 Dec 11;288(5791):613–615. doi: 10.1038/288613a0. [DOI] [PubMed] [Google Scholar]
  34. Starke K., Reimann W., Zumstein A., Hertting G. Effect of dopamine receptor agonists and antagonists on release of dopamine in the rabbit caudate nucleus in vitro. Naunyn Schmiedebergs Arch Pharmacol. 1978 Oct;305(1):27–36. doi: 10.1007/BF00497003. [DOI] [PubMed] [Google Scholar]
  35. Summy-Long J. Y., Keil L. C., Deen K., Severs W. B. Opiate regulation of angiotensin-induced drinking and vasopressin release. J Pharmacol Exp Ther. 1981 Jun;217(3):630–637. [PubMed] [Google Scholar]
  36. Vemes I., Mulder G. H., Smelik P. G., Tilders F. J. Differential control of beta-endorphin/beta-lipotropin secretion from anterior and intermediate lobes of the rat pituitary gland in vitro. Life Sci. 1980 Nov 10;27(19):1761–1768. doi: 10.1016/0024-3205(80)90443-9. [DOI] [PubMed] [Google Scholar]
  37. Wakerley J. B., Poulain D. A., Brown D. Comparison of firing patterns in oxytocin- and vasopressin-releasing neurones during progressive dehydration. Brain Res. 1978 Jun 16;148(2):425–440. doi: 10.1016/0006-8993(78)90730-8. [DOI] [PubMed] [Google Scholar]
  38. Watson S. J., Akil H., Ghazarossian V. E., Goldstein A. Dynorphin immunocytochemical localization in brain and peripheral nervous system: preliminary studies. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1260–1263. doi: 10.1073/pnas.78.2.1260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Weber E., Evans C. J., Chang J. K., Barchas J. D. Antibodies specific for alpha-N-acetyl-beta-endorphins: radioimmunoassays and detection of acetylated beta-endorphins in pituitary extracts. J Neurochem. 1982 Feb;38(2):436–447. doi: 10.1111/j.1471-4159.1982.tb08648.x. [DOI] [PubMed] [Google Scholar]

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