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. 1967 Oct;105(1):155–161. doi: 10.1042/bj1050155

Alkaline inorganic pyrophosphatase activity of mammalian-cell alkaline phosphatase

Rody P Cox 1, Paul Gilbert Jun 1, Martin J Griffin 1,*
PMCID: PMC1198286  PMID: 4964763

Abstract

Alkaline phosphatase prepared from mammalian cell cultures was found to have alkaline inorganic pyrophosphatase activity. Both of these activities appear to be associated with a single protein, as demonstrated by: (1) concomitant purification of alkaline phosphatase and alkaline inorganic pyrophosphatase; (2) proportional precipitation of alkaline phosphatase and inorganic pyrophosphatase activities by titrating constant amounts of an enzyme preparation with increasing concentration of antibody; (3) immune electrophoresis, which showed that precipitin bands that have alkaline phosphatase activity also have pyrophosphatase activity; (4) inhibition of pyrophosphatase activity by cysteine, an inhibitor of alkaline phosphatase activity; (5) similar subcellular localization of the two enzyme activities as demonstrated by histochemical methods; (6) hormonal and substrate induction of alkaline phosphatase activity in mammalian cell cultures, which produced a nearly parallel rise in inorganic pyrophosphatase activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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