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. 1967 Nov;105(2):497–503. doi: 10.1042/bj1050497

Phosphorylation of choline and ethanolamine in Ehrlich ascites-carcinoma cells

Cheng-Po Sung 1, R M Johnstone 1
PMCID: PMC1198337  PMID: 5626092

Abstract

1. Ehrlich ascites-cell extracts convert choline and ethanolamine approximately equally well into their respective phosphoryl derivatives. 2. Choline is a potent inhibitor of ethanolamine phosphorylation, but ethanolamine has little effect on choline phosphorylation. 3. 2,3-Dimercaptopropanol, cysteine and Ca2+ inhibit ethanolamine phosphorylation, but have no detectable effect on choline phosphorylation. 4. Choline-phosphorylating activity in Ehrlich ascites-cell extracts is more stable during storage than ethanolamine-phosphorylating activity. 5. Choline phosphorylation is stimulated in the presence of benzoylcholine, succinylcholine, butyrylcholine and propionylcholine, whereas ethanolamine phosphorylation is inhibited. This relationship is reciprocal: the compounds causing the greatest stimulation of choline phosphorylation bring about the greatest inhibition of ethanolamine phosphorylation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BREGOFF H. M., ROBERTS E., DELWICHE C. C. Paper chromatography of quaternary ammonium bases and related compounds. J Biol Chem. 1953 Dec;205(2):565–574. [PubMed] [Google Scholar]
  2. HERSCOVICS A., JOHNSTONE R. M. MECHANISM OF ACTION OF GLUCOSE AND PYRIMIDINE NUCLEOSIDES ON (14C)FORMATE UTILIZATION BY EHRLICH ASCITES CELLS. Biochim Biophys Acta. 1964 Nov 15;91:365–373. doi: 10.1016/0926-6550(64)90065-9. [DOI] [PubMed] [Google Scholar]
  3. Johnstone R. M. Factors affecting the incorporation of precursors into the phospholipids of Ehrlich ascites cells. Can J Biochem. 1967 Aug;45(8):1233–1243. doi: 10.1139/o67-144. [DOI] [PubMed] [Google Scholar]
  4. RAMASARMA T., WETTER L. R. Choline kinase of rapeseed (Brassica campestris L.). Can J Biochem Physiol. 1957 Oct;35(10):853–863. [PubMed] [Google Scholar]
  5. Sheltawy A., Dawson R. M. The polyphosphoinositides and other lipids of peripheral nerves. Biochem J. 1966 Jul;100(1):12–18. doi: 10.1042/bj1000012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Sung C. P., Johnstone R. M. Evidence for active transport of choline in rat kidney cortex slices. Can J Biochem. 1965 Jul;43(7):1111–1118. doi: 10.1139/o65-124. [DOI] [PubMed] [Google Scholar]
  7. WILGRAM G. F., KENNEDY E. P. INTRACELLULAR DISTRIBUTION OF SOME ENZYMES CATALYZING REACTIONS IN THE BIOSYNTHESIS OF COMPLEX LIPIDS. J Biol Chem. 1963 Aug;238:2615–2619. [PubMed] [Google Scholar]
  8. WITTENBERG J., KORNBERG A. Choline phosphokinase. J Biol Chem. 1953 May;202(1):431–444. [PubMed] [Google Scholar]

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