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. 1968 Jan;106(1):271–277. doi: 10.1042/bj1060271

Precursors of the pyrimidine moiety of thiamine

P C Newell 1,*, R G Tucker 1
PMCID: PMC1198495  PMID: 4889363

Abstract

1. A method was devised for obtaining the pyrimidine moiety of thiamine in a pure form after its excretion into the medium by de-repressed washed-cell suspensions of mutants of Salmonella typhimurium LT2. 2. By using amino acid-requiring mutants, this excretion of pyrimidine moiety was shown to be dependent on the presence of both methionine and glycine. 3. In the presence of either [Me-14C]methionine or [G-14C]methionine, methionine-requiring mutants did not incorporate radioactivity into the pyrimidine moiety. 4. In contrast, both [1-14C]glycine and [2-14C]glycine were incorporated into the pyrimidine moiety excreted by glycine-requiring mutants, and this occurred with little or no dilution of specific radioactivity. 5. The possible requirement for methionine as a cofactor and the significance of the incorporation of both carbon atoms of glycine are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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