Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1968 Jan;106(2):425–430. doi: 10.1042/bj1060425

Hydrocortisone requirement for the induction of glutamine synthetase in chick-embryo retinas

Liane Reif-Lehrer 1,*, Harold Amos 1
PMCID: PMC1198519  PMID: 5637352

Abstract

Hydrocortisone has been found to induce glutamine synthetase activity in chick-embryo retinas in culture. Evidence is presented to show that the hydrocortisone is definitely required for transcription; its requirement for translation has not been ruled out. The possible identity of hydrocortisone with an active component of calf-serum diffusate reported earlier is discussed. The data also indicate that the glutamine synthetase messenger RNA is stable for at least several hours.

Full text

PDF
426

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COLOMBO B., FELICETTI L., BAGLIONI C. INHIBITION OF PROTEIN SYNTHESIS BY CYCLOHEXIMIDE IN RABBIT RETICULOCYTES. Biochem Biophys Res Commun. 1965 Feb 3;18:389–395. doi: 10.1016/0006-291x(65)90719-9. [DOI] [PubMed] [Google Scholar]
  2. DARKEN M. A. PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS. Pharmacol Rev. 1964 Sep;16:223–243. [PubMed] [Google Scholar]
  3. DAUGHADAY W. H. Binding of corticosteroids by plasma proteins. IV. The electrophoretic demonstration of corticosteroid binding globulin. J Clin Invest. 1958 Apr;37(4):519–523. doi: 10.1172/JCI103633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. ENNIS H. L., LUBIN M. CYCLOHEXIMIDE: ASPECTS OF INHIBITION OF PROTEIN SYNTHESIS IN MAMMALIAN CELLS. Science. 1964 Dec 11;146(3650):1474–1476. doi: 10.1126/science.146.3650.1474. [DOI] [PubMed] [Google Scholar]
  5. KIRK D. L., MOSCONA A. A. SYNTHESIS OF EXPERIMENTALLY INDUCED GLUTAMINE SYNTHETASE (GLUTAMOTRANSFERASE ACTIVITY) IN EMBRYONIC CHICK RETINA IN VITRO. Dev Biol. 1963 Dec;8:341–357. doi: 10.1016/0012-1606(63)90034-4. [DOI] [PubMed] [Google Scholar]
  6. Kenney F. T., Albritton W. L. Repression of enzyme synthesis at the translational level and its hormonal control. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1693–1698. doi: 10.1073/pnas.54.6.1693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MOSCONA A. A., HUBBY J. L. Experimentally induced changes in glutamotransferase activity in embryonic tissue. Dev Biol. 1963 Mar;6:192–206. doi: 10.1016/0012-1606(63)90117-9. [DOI] [PubMed] [Google Scholar]
  9. MOSCONA A. A., KIRK D. L. CONTROL OF GLUTAMINE SYNTHETASE IN THE EMBRYONIC RETINA IN VITRO. Science. 1965 Apr 23;148(3669):519–521. doi: 10.1126/science.148.3669.519. [DOI] [PubMed] [Google Scholar]
  10. Melnykovych G. Glucocorticoid-induced resistance to deoxycholate lysis in Hela cells. Science. 1966 May 20;152(3725):1086–1087. doi: 10.1126/science.152.3725.1086. [DOI] [PubMed] [Google Scholar]
  11. Moscona A. A., Piddington R. Stimulation by hydrocortisone of premature changes in the developmental pattern of glutamine synthetase in embryonic retina. Biochim Biophys Acta. 1966 Jun 29;121(2):409–411. doi: 10.1016/0304-4165(66)90131-0. [DOI] [PubMed] [Google Scholar]
  12. PITOT H. C., PERAINO C., MORSE P. A., Jr, POTTER V. R. HEPATOMAS IN TISSUE CULTURE COMPARED WITH ADAPTING LIVER IN VIVO. Natl Cancer Inst Monogr. 1964 Apr;13:229–245. [PubMed] [Google Scholar]
  13. ROSEN F., NICHOL C. A. CORTICOSTEROIDS AND ENZYME ACTIVITY. Vitam Horm. 1963;21:135–214. doi: 10.1016/s0083-6729(08)60241-1. [DOI] [PubMed] [Google Scholar]
  14. Reif L., Amos H. A dialyzable inducer for the glutamotransferase of chick embryo retina. Biochem Biophys Res Commun. 1966 Apr 6;23(1):39–48. doi: 10.1016/0006-291x(66)90266-x. [DOI] [PubMed] [Google Scholar]
  15. SLAUNWHITE W. R., Jr, SANDBERG A. A. Transcortin: a corticosteroid-binding protein of plasma. J Clin Invest. 1959 Feb;38(2):384–391. doi: 10.1172/JCI103812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thompson E. B., Tomkins G. M., Curran J. F. Induction of tyrosine alpha-ketoglutarate transaminase by steroid hormones in a newly established tissue culture cell line. Proc Natl Acad Sci U S A. 1966 Jul;56(1):296–303. doi: 10.1073/pnas.56.1.296. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES