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. 1968 Feb;106(3):593–600. doi: 10.1042/bj1060593

The estimation of galactose, mannose and fucose in glycoproteins by radioisotope dilution

E R B Graham 1, A Neuberger 1
PMCID: PMC1198547  PMID: 4170711

Abstract

1. The principle of radioisotope dilution, as used previously for the estimation of mannose in egg albumin, was applied on a semi-micro scale to the estimation of fucose, mannose and galactose in some glycoproteins. The sugars were separated by partition chromatography on columns of Celite 545. 2. The release of mannose from egg albumin in 2n-hydrochloric acid at 100° after various times was determined by the radioisotope-dilution method and found to have a half-time of 7min. 3. The destruction of mannose in 2n-hydrochloric acid after 3hr. at 100° was found to be small if air was excluded. The destruction was slightly increased by the presence of lysozyme containing tryptophan in an amount equimolar with the mannose. The same amount of free tryptophan caused considerable loss of mannose. 4. Analytical values are reported for the non-amino sugar contents of egg albumin, rabbit γ-globulin and some samples of blood-group-specific substances. The values found were similar to the most reliable estimates published previously.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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