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. 1968 Mar;107(2):207–215. doi: 10.1042/bj1070207

The stepwise removal of histones from chicken erythrocyte nucleoprotein

K Murray 1,2,*, G Vidali 1,2, J M Neelin 1,2
PMCID: PMC1198647  PMID: 5641876

Abstract

1. A fractionation of chicken erythrocyte histones was achieved simultaneously with their extraction from saline-washed nuclei by stepwise titrations to progressively lower pH values. 2. Different acids and dilute buffer solutions of comparable pH behaved similarly in stepwise extractions of histones. 3. The histone preparations so obtained were characterized by their amino acid composition and behaviour on zone electrophoresis in starch gels. 4. The fractionation by titration was quite sharp at appropriate pH ranges, and the histone fraction that is apparently unique to avian erythrocytes was obtained without contamination by other histone fractions. 5. Histones prepared by stepwise titration were fractionated further by cation-exchange and exclusion chromatography. The chromatographic behaviour and amino acid composition of the components permitted comparison with histones prepared by other methods. 6. Histone fraction IIb was resolved into its subfractions IIb1 and IIb2 by exclusion chromatography on Bio-Gel P-60. 7. Histone fractions III and IV, previously reported to be absent from chicken erythrocyte nuclei, were found in extracts made at pH1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIJVOET P. Ethanol precipitation analysis of thymus histone. Biochim Biophys Acta. 1957 Sep;25(3):502–512. doi: 10.1016/0006-3002(57)90520-6. [DOI] [PubMed] [Google Scholar]
  2. Bellair J. T., Mauritzen C. M. Comparison of beta-histone isolated from chicken erythrocyte, liver and spleen, and ox thymus. Biochim Biophys Acta. 1967 Feb 21;133(2):263–271. doi: 10.1016/0005-2795(67)90066-9. [DOI] [PubMed] [Google Scholar]
  3. DALY M. M., MIRSKY A. E. Histones with high lysine content. J Gen Physiol. 1955 Jan 20;38(3):405–413. doi: 10.1085/jgp.38.3.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hnilica L. S., Bess L. G. The heterogeneity of arginine-rich histones. Anal Biochem. 1965 Sep;12(3):421–436. doi: 10.1016/0003-2697(65)90209-5. [DOI] [PubMed] [Google Scholar]
  5. Hnilica L. S. The specificity of histones in chicken erythrocytes. Experientia. 1964 Jan 15;20(1):13–14. doi: 10.1007/BF02146014. [DOI] [PubMed] [Google Scholar]
  6. JOHNS E. W., BUTLER J. A. Further fractionations of histones from calf thymus. Biochem J. 1962 Jan;82:15–18. doi: 10.1042/bj0820015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JOHNSON L. D., DRIEDGER A., MARKO A. M. CHROMATOGRAPHIC BEHAVOR OF HISTONES ON SEPHADEX AND CARBOXYMETHYLCELLULOSE. Can J Biochem. 1964 Jun;42:795–811. doi: 10.1139/o64-091. [DOI] [PubMed] [Google Scholar]
  8. Johns E. W. Studies on histones. 7. Preparative methods for histone fractions from calf thymus. Biochem J. 1964 Jul;92(1):55–59. doi: 10.1042/bj0920055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kinkade J. M., Jr, Cole R. D. The resolution of four lysine-rich histones derived from calf thymus. J Biol Chem. 1966 Dec 25;241(24):5790–5797. [PubMed] [Google Scholar]
  10. MURRAY K. A continuous elution method of preparative starch gel electrophoresis. Anal Biochem. 1962 May;3:415–428. doi: 10.1016/0003-2697(62)90070-2. [DOI] [PubMed] [Google Scholar]
  11. Murray K., Milstein C. Esters of serine and threonine in hydrolysates of histones and protamines, and attendant errors in amino acid analyses of proteins. Biochem J. 1967 Nov;105(2):491–495. doi: 10.1042/bj1050491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murray K. The acid extraction of histones from calf thymus deoxyribonucleoprotein. J Mol Biol. 1966 Feb;15(2):409–419. doi: 10.1016/s0022-2836(66)80116-x. [DOI] [PubMed] [Google Scholar]
  13. NEELIN J. M., BUTLER G. C. A comparison of histones from chicken tissues by zone electrophoresis in starch gel. Can J Biochem Physiol. 1961 Mar;39:485–491. doi: 10.1139/o61-046. [DOI] [PubMed] [Google Scholar]
  14. NEELIN J. M., CALLAHAN P. X., LAMB D. C., MURRAY K. THE HISTONES OF CHICKEN ERYTHROCYTE NUCLEI. Can J Biochem. 1964 Dec;42:1743–1752. doi: 10.1139/o64-185. [DOI] [PubMed] [Google Scholar]
  15. NEELIN J. M., CONNELL G. E. Zone electrophoresis of chicken-erythrocyte histone in starch gel. Biochim Biophys Acta. 1959 Feb;31(2):539–541. doi: 10.1016/0006-3002(59)90030-7. [DOI] [PubMed] [Google Scholar]
  16. PHILLIPS D. M., JOHNS E. W. A FRACTIONATION OF THE HISTONES OF GROUP F2A FROM CALF THYMUS. Biochem J. 1965 Jan;94:127–130. doi: 10.1042/bj0940127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Purkayastha R., Neelin J. M. Comparison of histones from avian erythroid tissues by zone electrophoresis. Biochim Biophys Acta. 1966 Oct 31;127(2):468–477. doi: 10.1016/0304-4165(66)90400-4. [DOI] [PubMed] [Google Scholar]
  18. de NOOIJ E., WESTENBRINK H. G. Isolation of a homogeneous lysine-rich histone from calf thymus. Biochim Biophys Acta. 1962 Aug 27;62:608–609. doi: 10.1016/0006-3002(62)90254-8. [DOI] [PubMed] [Google Scholar]

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