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. 1968 Apr;107(3):329–333. doi: 10.1042/bj1070329

The lipid composition of Mycoplasma laidlawii strain B

N Shaw 1,2, P F Smith 1,2, W L Koostra 1,2
PMCID: PMC1198667  PMID: 4297044

Abstract

1. Total lipid was extracted from Mycoplasma laidlawii strain B with chloroform–methanol mixtures and fractionated into neutral lipid, glycolipid and phospholipid components by chromatography on silicic acid. 2. Saponification of the glycolipid fraction, which represented nearly half of the total lipid, yielded two glycosides for which the structures O-α-d-glucopyranosyl-(1→1)-d-glycerol and O-α-d-glucopyranosyl-(1→2)-O-α-d-glucopyranosyl-(1→1)-d-glycerol were established. 3. The ratio of monoglucosyl diglyceride to diglucosyl diglyceride increased with the age of the culture, though the total glycolipid concentration remained virtually constant. The glycolipid concentration was unaffected by the addition of cholesterol to the culture medium. 4. The phospholipid fraction consisted of two components, phosphatidylglucose and phosphatidylglycerol. Organisms harvested at acidic pH also contained O-amino acyl esters of phosphatidylglycerol. No lipids containing inositol could be detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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