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. 1968 Apr;107(4):481–489. doi: 10.1042/bj1070481

The effect of ribonuclease on polysomes and ribosomes of bacteria and animal cells

M L Fenwick 1
PMCID: PMC1198691  PMID: 4173471

Abstract

The mildest treatment with ribonuclease that causes any disaggregation of the polysomes of Escherichia coli or HeLa cells simultaneously attacks the RNA of the constituent ribosomes. It is concluded that the susceptibility to ribonuclease of polysomes does not suggest that they are held together by a strand of messenger RNA. The RNA of the larger sub-unit of bacterial ribosomes has particularly sensitive regions resulting in a non-random degradation. The RNA of the smaller sub-unit of E. coli ribosomes is relatively resistant to ribonuclease attack. The same may be true of the respective sub-units of the intact HeLa-cell ribosome, but both sub-units become very sensitive to ribonuclease on dissociation from each other.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Capecchi M. R. Cell-free protein synthesis programmed with R17 RNA: identification of two phage proteins. J Mol Biol. 1966 Oct 28;21(1):173–193. doi: 10.1016/0022-2836(66)90086-6. [DOI] [PubMed] [Google Scholar]
  2. Capecchi M. R., Gussin G. N. Suppression in vitro: Identification of a Serine-sRNA as a "Nonsense" Suppressor. Science. 1965 Jul 23;149(3682):417–422. doi: 10.1126/science.149.3682.417. [DOI] [PubMed] [Google Scholar]
  3. Carter W. A., Levy H. B. Ribosomes: effect of interferon on their interaction with rapidly labeled cellular and viral RNA's. Science. 1967 Mar 10;155(3767):1254–1257. doi: 10.1126/science.155.3767.1254. [DOI] [PubMed] [Google Scholar]
  4. DRESDEN M., HOAGLAND M. B. POLYRIBOSOMES FROM ESCHERICHIA COLI: ENZYMATIC METHOD FOR ISOLATION. Science. 1965 Aug 6;149(3684):647–649. doi: 10.1126/science.149.3684.647. [DOI] [PubMed] [Google Scholar]
  5. Drach J. C., Lingrel J. B. Function of reticulocyte ribonucleic acid in the Escherichia coli cell-free system. Biochim Biophys Acta. 1966 Oct 24;129(1):178–185. doi: 10.1016/0005-2787(66)90019-0. [DOI] [PubMed] [Google Scholar]
  6. Dresden M. H., Hoagland M. B. Polyribosomes of Escherichia coli. Breakdown during glucose starvation. J Biol Chem. 1967 Mar 10;242(5):1065–1068. [PubMed] [Google Scholar]
  7. ERIKSON R. L., FENWICK M. L., FRANKLIN R. M. REPLICATION OF BACTERIOPHAGE RNA: STUDIES ON THE FATE OF PARENTAL RNA. J Mol Biol. 1964 Dec;10:519–529. doi: 10.1016/s0022-2836(64)80070-x. [DOI] [PubMed] [Google Scholar]
  8. FRAENKEL-CONRAT H., SINGER B., TSUGITA A. Purification of viral RNA by means of bentonite. Virology. 1961 May;14:54–58. doi: 10.1016/0042-6822(61)90131-3. [DOI] [PubMed] [Google Scholar]
  9. Godson G. N., Sinsheimer R. L. The replication of bacteriophage MS2. VI. Interaction between bacteriophage RNA and cellular components in MS2-infected Escherichia coli. J Mol Biol. 1967 Feb 14;23(3):495–521. doi: 10.1016/s0022-2836(67)80121-9. [DOI] [PubMed] [Google Scholar]
  10. Gould H. J., Arnstein H. R., Cox R. A. The dissociation of reticulocyte polysomes into subunits and the location of messenger RNA. J Mol Biol. 1966 Feb;15(2):600–618. doi: 10.1016/s0022-2836(66)80130-4. [DOI] [PubMed] [Google Scholar]
  11. HARRIS H., WATTS J. W. The relationship between nuclear and cytoplasmic ribonucleic acid. Proc R Soc Lond B Biol Sci. 1962 May 15;156:109–121. doi: 10.1098/rspb.1962.0031. [DOI] [PubMed] [Google Scholar]
  12. Hadjiolov A. A. An endomessenger hypothesis on the mechanism of information transfer in animal cells. J Theor Biol. 1967 Aug;16(2):229–250. doi: 10.1016/0022-5193(67)90006-9. [DOI] [PubMed] [Google Scholar]
  13. Hotham-Iglewski B., Franklin R. M. Replication of bacteriophage ribonucleic acid: alterations in polyribosome patterns and association of double-stranded RNA with polyribosomes in Escherichia coli infected with bacteriophage R17. Proc Natl Acad Sci U S A. 1967 Aug;58(2):743–749. doi: 10.1073/pnas.58.2.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Huez G., Burny A., Marbaix G., Lebleu B. Release of messenger RNA from rabbit reticulocyte polyribosomes at low concentration of divalent cations. Biochim Biophys Acta. 1967;145(3):629–636. doi: 10.1016/0005-2787(67)90122-0. [DOI] [PubMed] [Google Scholar]
  15. Hunt J. A., Wilkinson B. R. Stimulatory activity of high molecular weight ribonucleic acid in the rabbit reticulocyte cell-free system. Biochemistry. 1967 Jun;6(6):1688–1693. doi: 10.1021/bi00858a017. [DOI] [PubMed] [Google Scholar]
  16. Joklik W. K., Merigan T. C. Concerning the mechanism of action of interferon. Proc Natl Acad Sci U S A. 1966 Aug;56(2):558–565. doi: 10.1073/pnas.56.2.558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. KIHO Y., RICH A. INDUCED ENZYME FORMED ON BACTERIAL POLYRIBOSOMES. Proc Natl Acad Sci U S A. 1964 Jan;51:111–118. doi: 10.1073/pnas.51.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kiho Y., Rich A. A polycistronic messenger RNA associated with beta-galactosidase induction. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1751–1758. doi: 10.1073/pnas.54.6.1751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mangiarotti G., Schlessinger D. Polyribosome metabolism in Escherichia coli. I. Extraction of polyribosomes and ribosomal subunits from fragile, growing Escherichia coli. J Mol Biol. 1966 Sep;20(1):123–143. doi: 10.1016/0022-2836(66)90122-7. [DOI] [PubMed] [Google Scholar]
  20. Marbaix G., Burny A. Separation of the messenger RNA of reticulocyte polyribosomes. Biochem Biophys Res Commun. 1964 Aug 11;16(6):522–527. doi: 10.1016/0006-291x(64)90186-x. [DOI] [PubMed] [Google Scholar]
  21. Marcus P. I., Salb J. M. Molecular basis of interferon action: inhibition of viral RNA translation. Virology. 1966 Nov;30(3):502–516. doi: 10.1016/0042-6822(66)90126-7. [DOI] [PubMed] [Google Scholar]
  22. NATHANS D., NOTANI G., SCHWARTZ J. H., ZINDER N. D. Biosynthesis of the coat protein of coliphage f2 by E. coli extracts. Proc Natl Acad Sci U S A. 1962 Aug;48:1424–1431. doi: 10.1073/pnas.48.8.1424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Nair K. G., Zak R., Rabinowitz M. Studies of the effect of proteolytic enzymes on ribosomes and polysomes from reticulocytes and rat liver. Biochemistry. 1966 Aug;5(8):2674–2680. doi: 10.1021/bi00872a028. [DOI] [PubMed] [Google Scholar]
  24. Nanninga N. Fine structure observed in 50S ribosomal subunits of Bacillus subtilis. J Cell Biol. 1967 May;33(2):C1–C6. doi: 10.1083/jcb.33.2.c1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Nathans D. Cell-free protein synthesis directed by coliphage MS2 RNA: synthesis of intact viral coat protein and other products. J Mol Biol. 1965 Sep;13(2):521–531. doi: 10.1016/s0022-2836(65)80114-0. [DOI] [PubMed] [Google Scholar]
  26. Oxman M. N., Baron S., Black P. H., Takemoto K. K., Habel K., Rowe W. P. The effect of interferon on SV-40 T antigen production in SV-40-transformed cells. Virology. 1967 May;32(1):122–127. doi: 10.1016/0042-6822(67)90260-7. [DOI] [PubMed] [Google Scholar]
  27. PENMAN S., BECKER Y., DARNELL J. E. A CYTOPLASMIC STRUCTURE INVOLVED IN THE SYNTHESIS AND ASSEMBLY OF POLIOVIRUS COMPONENTS. J Mol Biol. 1964 Apr;8:541–555. doi: 10.1016/s0022-2836(64)80010-3. [DOI] [PubMed] [Google Scholar]
  28. Penman S., Scherrer K., Becker Y., Darnell J. E. POLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA. Proc Natl Acad Sci U S A. 1963 May;49(5):654–662. doi: 10.1073/pnas.49.5.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. RABINOWITZ M., ZAK R., BELLER B., RAMPERSAD O., WOOL I. G. EFFECT OF PROTEOLYTIC ENZYMES ON SEDIMENTATION PROPERTIES OF RIBONUCLEOPROTEIN PARTICLES FROM HEART MUSCLE. Proc Natl Acad Sci U S A. 1964 Dec;52:1353–1360. doi: 10.1073/pnas.52.6.1353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. SANTER M. RIBOSOMAL RNA ON THE SURFACE OF RIBOSOMES. Science. 1963 Sep 13;141(3585):1049–1050. doi: 10.1126/science.141.3585.1049. [DOI] [PubMed] [Google Scholar]
  31. SCHLESSINGER D., MARCHESTI V. T., KWAN B. C. BINDING OF RIBOSOMES TO CYTOPLASMIC RETICULUM OF BACILLUS MEGATERIUM. J Bacteriol. 1965 Aug;90:456–466. doi: 10.1128/jb.90.2.456-466.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. SHAKULOV R. S., AITKHOZHIN M. A., SPIRIN A. S. [On latent degradation of ribosomes]. Biokhimiia. 1962 Jul-Aug;27:744–751. [PubMed] [Google Scholar]
  33. SPIRIN A. S., KISELEV N. A., SHAKULOV R. S., BOGDANOV A. A. IZUCHENIE STRUKTURY RIBOSOM; OBRATIMOE RAZVORACHIVANIE RIBOSOMNYKH CHASTITS V RIBONUKLEOPROTEIDNYE TIAZHI I MODEL' UKLADKI. Biokhimiia. 1963 Sep-Oct;28:920–930. [PubMed] [Google Scholar]
  34. Salser W., Gesteland R. F., Bolle A. In vitro synthesis of bacteriophage lysozyme. Nature. 1967 Aug 5;215(5101):588–591. doi: 10.1038/215588a0. [DOI] [PubMed] [Google Scholar]
  35. Santer M., Smith J. R. Ribonuclease sensitivity of Escherichia coli ribosomes. J Bacteriol. 1966 Oct;92(4):1099–1110. doi: 10.1128/jb.92.4.1099-1110.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Singer M. F., Leder P. Messenger RNA: an evaluation. Annu Rev Biochem. 1966;35:195–230. doi: 10.1146/annurev.bi.35.070166.001211. [DOI] [PubMed] [Google Scholar]
  37. Tsiapalis C. M., Hayashi Y., Chefurka W. Polyribosomes from houseflies. Nature. 1967 Apr 22;214(5086):358–361. doi: 10.1038/214358a0. [DOI] [PubMed] [Google Scholar]
  38. Vaughan M. H., Warner J. R., Darnell J. E. Ribosomal precursor particles in the HeLa cell nucleus. J Mol Biol. 1967 Apr 28;25(2):235–251. doi: 10.1016/0022-2836(67)90140-4. [DOI] [PubMed] [Google Scholar]
  39. Wiesner R., Acs G., Reich E., Shafiq A. Degradation of ribonucleic acid in mouse fibroblasts treated with actinomycin. J Cell Biol. 1965 Oct;27(1):47–52. doi: 10.1083/jcb.27.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. van Iterson W. The fine structure of the ribonucleoprotein in bacterial cytoplasm. J Cell Biol. 1966 Mar;28(3):563–570. doi: 10.1083/jcb.28.3.563. [DOI] [PMC free article] [PubMed] [Google Scholar]

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