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. 1968 Apr;107(4):565–574. doi: 10.1042/bj1070565

Amino acid uptake in the developing chick embryo heart. The effect of insulin on α-aminoisobutyric acid accumulation

G G Guidotti 1, A F Borghetti 1, G Gaja 1, L Lo Reti 1, G Ragnotti 1, P P Foà 1
PMCID: PMC1198700  PMID: 5660636

Abstract

1. The uptake of 14C-labelled α-aminoisobutyric acid by 5-day-old chick embryo hearts was investigated in vitro, together with the effect of insulin thereon. 2. At equilibrium the distribution ratio of this amino acid analogue between intracellular and extracellular water attained values greater than unity. Insulin enhanced the rate of α-aminoisobutyric acid accumulation and increased the value of its final concentration in the cell water. 3. The rate of α-aminoisobutyric acid accumulation and the effect of insulin on it were independent of the presence of glucose in the incubation medium. Bovine and chicken insulin were equally effective, and the action of the hormone was specifically prevented by an anti-insulin serum but not by puromycin. 4. A linear relationship was observed between the intracellular accumulation of the analogue and the logarithm of the insulin concentration in the range 50μunits–100m-units/ml. of incubation medium. 5. Evidence was obtained for the occurrence of two different transport processes for α-aminoisobutyric acid in the chick embryo heart: one subject to saturation and one that was not saturated by reasonable concentrations of the analogue. Insulin increased the effectiveness of the saturable component, increasing the maximal velocity of transport without altering the concentration for half-maximal velocity of transport, and decreased the contribution of the non-saturable component.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AKEDO H., CHRISTENSEN H. N. Nature of insulin action on amino acid uptake by the isolated diaphragm. J Biol Chem. 1962 Jan;237:118–122. [PubMed] [Google Scholar]
  2. BERMAN M., SHAHN E., WEISS M. F. The routine fitting of kinetic data to models: a mathematical formalism for digital computers. Biophys J. 1962 May;2:275–287. doi: 10.1016/s0006-3495(62)86855-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BERMAN M., WEISS M. F., SHAHN E. Some formal approaches to the analysis of kinetic data in terms of linear compartmental systems. Biophys J. 1962 May;2:289–316. doi: 10.1016/s0006-3495(62)86856-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BLEEHEN N. M., FISHER R. B. The action of insulin in the isolated rat heart. J Physiol. 1954 Feb 26;123(2):260–276. doi: 10.1113/jphysiol.1954.sp005049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Christensen H. N., Liang M. On the nature of the "non-saturable" migration of amino acids into Ehrlich cells and into rat jejunum. Bibl Laeger. 1966 Mar 14;112(3):524–531. doi: 10.1016/0926-6585(66)90255-x. [DOI] [PubMed] [Google Scholar]
  6. Christensen H. N., Liang M. Transport of diamino acids into the Ehrlich cell. J Biol Chem. 1966 Dec 10;241(23):5542–5551. [PubMed] [Google Scholar]
  7. DEL MONTE U. Relations between active transport and metabolism in rat diaphragm in vitro. Biochim Biophys Acta. 1961 May 27;49:431–440. doi: 10.1016/0006-3002(61)90240-2. [DOI] [PubMed] [Google Scholar]
  8. DOWD J. E., RIGGS D. S. A COMPARISON OF ESTIMATES OF MICHAELIS-MENTEN KINETIC CONSTANTS FROM VARIOUS LINEAR TRANSFORMATIONS. J Biol Chem. 1965 Feb;240:863–869. [PubMed] [Google Scholar]
  9. FRITZ G. R., KNOBIL E. IN VITRO STIMULATION BY INSULIN OF ALPHA-AMINO-ISOBUTYRIC ACID TRANSPORT IN THE ABSENCE OF PROTEIN SYNTHESIS. Nature. 1963 Nov 16;200:682–682. doi: 10.1038/200682a0. [DOI] [PubMed] [Google Scholar]
  10. Foà P. P., Melli M., Berger C. K., Billinger D., Guidotti G. G. Action of insulin on chick embryo heart. Fed Proc. 1965 Sep-Oct;24(5):1046–1050. [PubMed] [Google Scholar]
  11. GUIDOTTI G., FOA P. P. Development of an insulin-sensitive glucose transport system in chick embryo hearts. Am J Physiol. 1961 Nov;201:869–872. doi: 10.1152/ajplegacy.1961.201.5.869. [DOI] [PubMed] [Google Scholar]
  12. GUIDOTTI G., KANAMEISHI D., FOA P. P. Chick embryo heart as a tool for studying cell permeability and insulin action. Am J Physiol. 1961 Nov;201:863–868. doi: 10.1152/ajplegacy.1961.201.5.863. [DOI] [PubMed] [Google Scholar]
  13. Guidotti G. G., Gaja G., Loreti L., Ragnotti G., Rottenberg D. A., Borghetti A. F. Amino acid uptake in the developing chick embryo heart. The effect of insulin on glycine and leucine accumulation. Biochem J. 1968 Apr;107(4):575–580. doi: 10.1042/bj1070575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Guidotti G. G., Loreti L., Gaja G., Foà P. P. Glucose uptake in the developing chick embryo heart. Am J Physiol. 1966 Oct;211(4):981–987. doi: 10.1152/ajplegacy.1966.211.4.981. [DOI] [PubMed] [Google Scholar]
  15. MANCHESTER K. L., WOOL I. G. INSULIN AND INCORPORATION OF AMINO ACIDS INTO PROTEIN OF MUSCLE. 1. ACCUMULATION AND INCORPORATION STUDIES WITH THE PERFUSED RAT HEART. Biochem J. 1963 Nov;89:202–209. doi: 10.1042/bj0890202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MANCHESTER K. L., YOUNG F. G. The effect of insulin in vitro on the accumulation of amino acids by isolated rat diaphragm. Biochem J. 1960 Jun;75:487–495. doi: 10.1042/bj0750487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MORGAN H. E., HENDERSON M. J., REGEN D. M., PARK C. R. Regulation of glucose uptake in muscle. I. The effects of insulin and anoxia on glucose transport and phosphorylation in the isolated, perfused heart of normal rats. J Biol Chem. 1961 Feb;236:253–261. [PubMed] [Google Scholar]
  18. NOALL M. W., RIGGS T. R., WALKER L. M., CHRISTENSEN H. N. Endocrine control of amino acid transfer; distribution of an unmetabolizable amino acid. Science. 1957 Nov 15;126(3281):1002–1005. doi: 10.1126/science.126.3281.1002. [DOI] [PubMed] [Google Scholar]
  19. Scharff R., Wool I. G. Accumulation of amino acids in muscle of perfused rat heart. Effect of insulin in the presence of puromycin. Biochem J. 1965 Oct;97(1):272–276. doi: 10.1042/bj0970272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scharff R., Wool I. G. Accumulation of amino acids in muscle of perfused rat heart. Effect of insulin. Biochem J. 1965 Oct;97(1):257–271. doi: 10.1042/bj0970257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. THOMMES R. C. A histochemical study of insulin in the chick embryo pancreas. Growth. 1960 Mar;24:69–80. [PubMed] [Google Scholar]
  22. Udenfriend S., Zaltzman-Nirenburg P., Guroff G. A study of cellular transport with the fluorescent amino acid, aminonaphthylalanine. Arch Biochem Biophys. 1966 Sep 26;116(1):261–270. doi: 10.1016/0003-9861(66)90032-4. [DOI] [PubMed] [Google Scholar]
  23. VALLANCE-OWEN J., HURLOCK B. Estimation of plasma-insulin by the rat diaphragm method. Lancet. 1954 Jan 9;266(6802):68–70. doi: 10.1016/s0140-6736(54)90820-x. [DOI] [PubMed] [Google Scholar]
  24. Wohltmann H. J., Narahara H. T. Binding of insulin-131-I by isolated frog sartorius muscles. Relationship to changes in permeability to sugar caused by insulin. J Biol Chem. 1966 Nov 10;241(21):4931–4939. [PubMed] [Google Scholar]
  25. Wool I. G., Castles J. J., Moyer A. N. Regulation of amino acid accumulation in isolated rat diagphragm: effect of puromycin and insulin. Biochim Biophys Acta. 1965 Sep 13;107(2):333–345. doi: 10.1016/0304-4165(65)90139-x. [DOI] [PubMed] [Google Scholar]

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