Abstract
The process of healing-over was followed in trabeculae from the right ventricle of guinea-pigs and in Purkinje fibres from dogs by measuring resting potential and input resistance close to a site of damage. In Ca2+-free bathing solution at pH 6.5 no signs of healing-over were found; at pH 5.5 healing-over was incomplete; at pH 5.0 and 4.0 healing-over occurred promptly. It is concluded that transformation of low-resistance nexus membranes into high-resistance membranes separating the intracellular from the extracellular compartment normally is due to Ca2+, while protons can bring about the same effect under extreme conditions.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- De Mello W. C. Effect of intracellular injection of calcium and strontium on cell communication in heart. J Physiol. 1975 Sep;250(2):231–245. doi: 10.1113/jphysiol.1975.sp011051. [DOI] [PMC free article] [PubMed] [Google Scholar]
- De Mello W. C. Influence of intracellular injection of H+ on the electrical coupling in cardiac Purkinje fibres. Cell Biol Int Rep. 1980 Jan;4(1):51–58. doi: 10.1016/0309-1651(80)90009-0. [DOI] [PubMed] [Google Scholar]
- Deitmer J. W., Ellis D. Interactions between the regulation of the intracellular pH and sodium activity of sheep cardiac Purkinje fibres. J Physiol. 1980 Jul;304:471–488. doi: 10.1113/jphysiol.1980.sp013337. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FATT P., KATZ B. An analysis of the end-plate potential recorded with an intracellular electrode. J Physiol. 1951 Nov 28;115(3):320–370. doi: 10.1113/jphysiol.1951.sp004675. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LING G., GERARD R. W. The normal membrane potential of frog sartorius fibers. J Cell Physiol. 1949 Dec;34(3):383–396. doi: 10.1002/jcp.1030340304. [DOI] [PubMed] [Google Scholar]
- Nishiye H. The mechanism of Ca2+ action on the healing-over process in mammalian cardiac muscles: a kinetic analysis. Jpn J Physiol. 1977;27(4):451–466. doi: 10.2170/jjphysiol.27.451. [DOI] [PubMed] [Google Scholar]
- Reber W. R., Weingart R. Ungulate cardiac purkinje fibres: the influence of intracellular pH on the electrical cell-to-cell coupling. J Physiol. 1982 Jul;328:87–104. doi: 10.1113/jphysiol.1982.sp014254. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rose B., Rick R. Intracellular pH, intracellular free Ca, and junctional cell-cell coupling. J Membr Biol. 1978 Dec 29;44(3-4):377–415. doi: 10.1007/BF01944230. [DOI] [PubMed] [Google Scholar]
- Spray D. C., Stern J. H., Harris A. L., Bennett M. V. Gap junctional conductance: comparison of sensitivities to H and Ca ions. Proc Natl Acad Sci U S A. 1982 Jan;79(2):441–445. doi: 10.1073/pnas.79.2.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turin L., Warner A. E. Intracellular pH in early Xenopus embryos: its effect on current flow between blastomeres. J Physiol. 1980 Mar;300:489–504. doi: 10.1113/jphysiol.1980.sp013174. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turin L., Warner A. Carbon dioxide reversibly abolishes ionic communication between cells of early amphibian embryo. Nature. 1977 Nov 3;270(5632):56–57. doi: 10.1038/270056a0. [DOI] [PubMed] [Google Scholar]
- WEIDMANN S. The electrical constants of Purkinje fibres. J Physiol. 1952 Nov;118(3):348–360. doi: 10.1113/jphysiol.1952.sp004799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Mello W. C., Motta G. E., Chapeau M. A study on the healing-over of myocardial cells of toads. Circ Res. 1969 Mar;24(3):475–487. doi: 10.1161/01.res.24.3.475. [DOI] [PubMed] [Google Scholar]